“…For Safranin, the absorbance peaks observed were mainly aliphatic primary amine N–H stretching (3,000–3,560 cm −1 ) and –OH stretching in the range of 3,589–4,000 nm in the control sample. However, in the decolorized test sample, the absorbance peaks varied: C–L stretching at 625 nm, C–N stretching 1,042–1,626 nm, C–C stretching 1,626 nm, C–H stretching 2,853–3,243 nm, and –OH stretching 3,283–3,564 nm ( Babu et al, 2015 ; Sahu and Patel, 2015 ). The shift in the peaks suggests a breakdown of functional groups that may have changed the original structure of the dyes in the decolorized test samples ( Chen et al, 2008 ; Ali et al, 2009 ).…”