Removal of Residual DNA and Host Cell Proteins for the Purification of Recombinant Staphylokinase Expressed in Escherichia coli
Li Guang,
Li jing,
Zou Zhenxing
et al.
Abstract:The elimination of residual host cell DNA (HCD) and proteins (HCPs) is a pivotal step in the purification process for biological products such as monoclonal antibodies, recombinant proteins, vaccines, gene therapy vectors, and cell‐based therapies. During the preparation of recombinant staphylokinase (r‐SAK), a potential therapeutic protein for thrombotic disorders expressed in Escherichia coli cells, an efficient chromatography purification process, incorporating anion exchange, cation exchange, and gel filtr… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.