Removal of polyphenolic compounds interfering with carbohydrate determination in plant extracts with an insoluble polyphenol adsorbent

Sanderson, Gary W.; Perera, B. P. M.

doi:10.1039/an9669100335

Cited by 18 publications

(7 citation statements)

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“…They are formed during fermentation by polymerization of theaflavins, which are formed during the manufacture of black tea from the enzymatic oxidation of catechins by polyphenol oxidase (Roberts 1958; Hazarika et al 1984). Polyphenols combine with proteins and perhaps also polysaccharides (Sanderson & Perera 1966; Haslam 1974). The thearubigin fraction used in the present study contains at least 80% polyphenols (% weight/extract weight).…”

Section: Discussionmentioning

confidence: 99%

The Mechanism Underlying the Protective Effect of the Thearubigin Fraction of Black Tea (Camellia sinensis) Extract against the Neuromuscular Blocking Action of Botulinum Neurotoxins

Satoh¹,

Ishii²,

Shimizu

et al. 2002

Pharmacology & Toxicology

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Abstract:The aim of the present study was to elucidate the mechanism of the protective effect of black tea extract, the thearubigin fraction, against the neuromuscular blocking action of botulinum neurotoxin types A, B, and E. The effects of thearubigin fraction extracted from a black tea infusion were examined on the neuromuscular blocking action of botulinum neurotoxin types A, B, and E in mouse phrenic nerve-diaphragm preparations and on the binding of these toxins to rat cerebrocortical synaptosomes. Botulinum neurotoxin type A (1.5 nM), B (6 nM), or E (5 nM) abolished indirect twitches in mouse phrenic nerve-diaphragm preparations within 50, 90, 90 min., respectively. Thearubigin fraction mixed with each toxin blocked the inhibitory effect of the toxins. The specific binding of [ 125 I]botulinum neurotoxin type A, B, or E to rat cerebrocortical synaptosomes was inhibited by mixing iodinated toxin with thearubigin fraction. The elution profile of [ 125 I]botulinum neurotoxin type A, B, or E on Sephadex G-50 column chromatography was different from that of toxin mixed with thearubigin fraction. These findings indicate that thearubigin fraction protects against the neuromuscular blocking action of botulinum neurotoxin types A, B, and E by binding with the toxins.

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Section: Discussionmentioning

confidence: 99%

The Mechanism Underlying the Protective Effect of the Thearubigin Fraction of Black Tea (Camellia sinensis) Extract against the Neuromuscular Blocking Action of Botulinum Neurotoxins

Satoh¹,

Ishii²,

Shimizu

et al. 2002

Pharmacology & Toxicology

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show abstract

“…The brown acidic pigments of black tea are classified as thearubigins and the yellow, neutral pigments are classified as theaflavins ( Roberts & Williams, 1958 ). Polyphenols can combine with proteins and perhaps polysaccharides as well ( Haslam, 1974 ; Sanderson & Perera, 1966 ). Thus, TRB might inactivate the toxins by possible binding manner.…”

Section: Discussionmentioning

confidence: 99%

Black tea extract, thearubigin fraction, counteract the effects of botulinum neurotoxins in mice

Satoh

Ishii

Shimizu

et al. 2001

British J Pharmacology

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Botulinum neurotoxin type A (BoNT/A, 1.5 nM) completely inhibited indirectly evoked twitches in in vitro mouse phrenic nerve-diaphragm preparations within 40 ± 45 min. Black tea extract, thearubigin fraction (TRB), mixed with BoNT/A blocked the inhibitory eect of the toxin. The protective eect of TRB extended to botulinum neurotoxins types B and E (BoNT/B and BoNT/E) and tetanus toxin, but not to tetrodotoxin. TRB was also eective against oral toxicity of BoNT/A, B and E. Thus, TRB may be of potential bene®t in protecting the paralytic actions of botulinum neurotoxins (BoNTs), but its use is limited by mixing with the toxin.

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“…After heating the PVP-adsorbate with n-methyl-2-pyrrolidone containing HCl and FeS04, the anthocyanidins in the supernatant were assayed colorimetrically. Sanderson et al (1966) have developed a method using insoluble PVP to remove catechins and related polyphenols which interfere with carbohydrate determinations in plant tissues. The polyphenols in the plant extracts were adsorbed on the PVP, and the carbohydrates were removed by centrifugation.…”

mentioning

confidence: 99%

Isolation of Strawberry Anthocyanin Pigments by Adsorption on Insoluble Polyvinylpyrrolidone

Wrolstad

Putnam

1969

Journal of Food Science

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Removal of polyphenolic compounds interfering with carbohydrate determination in plant extracts with an insoluble polyphenol adsorbent

Cited by 18 publications

References 0 publications

The Mechanism Underlying the Protective Effect of the Thearubigin Fraction of Black Tea (Camellia sinensis) Extract against the Neuromuscular Blocking Action of Botulinum Neurotoxins

The Mechanism Underlying the Protective Effect of the Thearubigin Fraction of Black Tea (Camellia sinensis) Extract against the Neuromuscular Blocking Action of Botulinum Neurotoxins

Black tea extract, thearubigin fraction, counteract the effects of botulinum neurotoxins in mice

Isolation of Strawberry Anthocyanin Pigments by Adsorption on Insoluble Polyvinylpyrrolidone

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