Removal of phenols-like substances in pharmaceutical wastewater with fungal bioreactors by adding Trametes versicolor

Abstract

Fungi are known to be more resistant to toxic compounds and more effective in removing recalcitrant organics such as phenols than bacteria. Here we examined the removal of phenols (as a component of Zopliclone drugs), added to non-sterile pharmaceutical wastewater with continuous treatment fungal bioreactor by its augmentation with mono-species of white-rot fungi (WRF) Trametes versicolor. Results showed that WRF in a sterile reactor (a batch mode) were moderately effective for removal of phenols (40% in seven days); however, native wastewater microbes at optimal conditions for fungi (pH 5.5, 25 °C) were more effective (90%, both in batch and continuous flow modes). In continuous flow mode, addition of WRF was an effective way to mitigate high loads of phenols (up to 400 mg/L), by both fungal enzymes (growth rate 0.075 h−1, laccase enzymatic activity 4 nkat/mL) and biosorption. The study confirmed that naturaly occuring fungi in combination with fungus-augmentation is an effective approach for treatment of high-strength pharmaceutical wastewater.

Fungal assisted bio‐treatment of environmental pollutants with comprehensive emphasis on noxious heavy metals: Recent updates

“…The removal of >98% of diclofenac in non‐sterile wastewater can be done by A. luchuensis as the results demonstrated (Dalecka et al, 2020). Bernats and Juhna (2018) examined the phenols elimination added to nonsterile pharmaceutical wastewater with continuous treatment fungal bioreactor by its augmentation with Trametes versicolor . In a sterile reactor (a batch mode), white rot fungi were temperately effective for phenols removal (40% in 7 days); though, at optimal conditions for fungi (pH 5.5, 25°C) native wastewater microbes were more operative (90%, both in batch and continuous flow modes) (Bernats & Juhna, 2018).…”

“…examined the phenols elimination added to nonsterile pharmaceutical wastewater with continuous treatment fungal bioreactor by its augmentation with Trametes versicolor. In a sterile reactor (a batch mode), white rot fungi were temperately effective for phenols removal (40% in 7 days); though, at optimal conditions for fungi (pH 5.5, 25°C) native wastewater microbes were more operative (90%, both in batch and continuous flow modes)(Bernats & Juhna, 2018) Lucas et al (2016). evaluated the capability of a fungal treatment for the removal of 47 antibiotics belonging to β-lactams, fluoroquinolones, macrolides, metronidazoles, sulfonamides, tetracyclines, and trimethoprim groups and the 77% antibiotics were found to be removed after fungal treatments.…”

“…Complete elimination (99.9%) of diclofenac from a synthetic wastewater media within 10 days of incubation can take place by the fungal isolate of Aspergillus luchuensis as indicated by the removal efficiency results. The removal of >98% of diclofenac in non-sterile wastewater can be done by A. luchuensis as the results demonstrated(Dalecka et al, 2020) Bernats and Juhna (2018). examined the phenols elimination added to nonsterile pharmaceutical wastewater with continuous treatment fungal bioreactor by its augmentation with Trametes versicolor.…”

Isolation of Fungal Strains from Municipal Wastewater for the Removal of Pharmaceutical Substances

“…Two additional negative controls-a control without chemical compounds and a control without fungi-were prepared to compare the experimental observations in order to be completely linked to the bioremoval induced by fungi. Finally, the samples for the HPLC and laccase activity analysis were taken at 0, 3, 6, 9, 12 and 24 h. The experiment was carried out in duplicate [17].…”

Produção de lacase para o pré-tratamento da digestão anaeróbia da água residuária do beneficiamento de café

“…Dentre as enzimas extracelulares produzidas por estes fungos para degradação da lignina e outros compostos, destacam-se as lacases, que vêm sendo aplicadas na degradação de diversos poluentes, como os corantes têxteis sintéticos (ALMEIDA et al, 2018;MUSSATTO, 2014), pentaclorofenol (COELHO et al, 2019 e compostos fenólicos (BERNATS;JUHNA, 2018;KŘESINOVÁ et al, 2018). Essa enzima catalisa a oxidação de seus substratos, com a concomitante redução de oxigênio molecular à água (MANAVALAN; MANAVALAN;HEESE, 2015).…”