Removal of Phenol in Batch Culture by Pseudomonas putida AP11, AP9, AP6 and AP7 Isolated from the Aromatic Hydrocarbon Contaminated Soils

Khan, Mohammed Abdullah; Fakhruddin, A. N. M.; Jame, Sadia Afrin; Sultana, Marjia; Alam, M. K.

doi:10.3329/jsr.v3i2.6339

Cited by 2 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of their abilities to use a wide diversity of carbon energy substrates, they can compete effectively with other bacteria and become dominant culturable members that are capable of utilizing the phenolic compounds found in contaminated sludge. 24 …”

Section: Discussionmentioning

confidence: 99%

Phenol degradation and genotypic analysis of dioxygenase genes in bacteria isolated from sediments

Tian

Zhou

et al. 2017

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

The aerobic degradation of aromatic compounds by bacteria is performed by dioxygenases. To show some characteristic patterns of the dioxygenase genotype and its degradation specificities, twenty-nine gram-negative bacterial cultures were obtained from sediment contaminated with phenolic compounds in Wuhan, China. The isolates were phylogenetically diverse and belonged to 10 genera. All 29 gram-negative bacteria were able to utilize phenol, m-dihydroxybenzene and 2-hydroxybenzoic acid as the sole carbon sources, and members of the three primary genera Pseudomonas, Acinetobacter and Alcaligenes were able to grow in the presence of multiple monoaromatic compounds. PCR and DNA sequence analysis were used to detect dioxygenase genes coding for catechol 1,2-dioxygenase, catechol 2,3-dioxygenase and protocatechuate 3,4-dioxygenase. The results showed that there are 4 genotypes; most strains are either PNP (catechol 1,2-dioxygenase gene is positive, catechol 2,3-dioxygenase gene is negative, protocatechuate 3,4-dioxygenase gene is positive) or PNN (catechol 1,2-dioxygenase gene is positive, catechol 2,3-dioxygenase gene is negative, protocatechuate 3,4-dioxygenase gene is negative). The strains with two dioxygenase genes can usually grow on many more aromatic compounds than strains with one dioxygenase gene. Degradation experiments using a mixed culture representing four bacterial genotypes resulted in the rapid degradation of phenol. Determinations of substrate utilization and phenol degradation revealed their affiliations through dioxygenase genotype data.

show abstract

Section: Discussionmentioning

confidence: 99%