Abstract:Abstract. Trinitrotoluene (TNT), a commonly used explosive for military and industrial applications, can cause serious environmental pollution. 28-day laboratory pot experiment was carried out applying bioaugmentation using laboratory selected bacterial strains as inoculum, biostimulation with molasses and cabbage leaf extract, and phytoremediation using rye and blue fenugreek to study the effect of these treatments on TNT removal and changes in soil microbial community responsible for contaminant degradation.… Show more
“…Pseudomonas sp. showed a 97.9% Pb, 93.5% Cd, and 68% Cu removal efficiency from contaminated industrial wastewater is reported [ 72 ]. Surface binding/reduced uptake, increased efflux intracellular sequestration, enzyme detoxication, and active transport are among the proven mechanisms of tolerance [ 73 ].…”
Drought stress (DS) is the most impacting global phenomenon affecting the ecological balance of a particular habitat. The search for potential plant growth-promoting rhizobacteria (PGPR) capable of enhancing plant tolerance to drought stress is needed. Thus, this study was initiated to evaluate the effect of inoculating Acacia abyssinica seedlings with PGPR isolated from rhizosphere soil of Ethiopia to enhance DS tolerance. The strains were selected based on in vitro assays associated with tolerance to drought and other beneficial traits such as salinity, acidity, temperature, heavy metal tolerances, biofilm formation, and exopolysaccharide (EPS) production. The strains with the best DS tolerance ability were selected for the greenhouse trials with acacia plants. The results indicate that out of 73 strains, 10 (14%) were completely tolerant to 40% polyethylene glycol. Moreover, 37% of the strains were strong biofilm producers, while 66 (90.41%) were EPS producers with a better production in the medium containing sucrose at 28 ± 2°C and pH 7 ± 0.2. Strains PS-16 and RS-79 showed tolerance to 11% NaCl. All the strains were able to grow in wider ranges of pH (4–10) and temperature (15–45°C) and had high tolerance to heavy metals. The inoculated bacterial strains significantly (
p
≤
0.05
) increased root and shoot length and dry biomass of acacia plants. One of the strains identified as P. fluorescens strain FB-49 was outstanding in enhancing DS tolerance compared to the single inoculants and comparable to consortia. Stress-tolerant PGPR could be used to enhance acacia DS tolerance after testing other phytobeneficial traits.
“…Pseudomonas sp. showed a 97.9% Pb, 93.5% Cd, and 68% Cu removal efficiency from contaminated industrial wastewater is reported [ 72 ]. Surface binding/reduced uptake, increased efflux intracellular sequestration, enzyme detoxication, and active transport are among the proven mechanisms of tolerance [ 73 ].…”
Drought stress (DS) is the most impacting global phenomenon affecting the ecological balance of a particular habitat. The search for potential plant growth-promoting rhizobacteria (PGPR) capable of enhancing plant tolerance to drought stress is needed. Thus, this study was initiated to evaluate the effect of inoculating Acacia abyssinica seedlings with PGPR isolated from rhizosphere soil of Ethiopia to enhance DS tolerance. The strains were selected based on in vitro assays associated with tolerance to drought and other beneficial traits such as salinity, acidity, temperature, heavy metal tolerances, biofilm formation, and exopolysaccharide (EPS) production. The strains with the best DS tolerance ability were selected for the greenhouse trials with acacia plants. The results indicate that out of 73 strains, 10 (14%) were completely tolerant to 40% polyethylene glycol. Moreover, 37% of the strains were strong biofilm producers, while 66 (90.41%) were EPS producers with a better production in the medium containing sucrose at 28 ± 2°C and pH 7 ± 0.2. Strains PS-16 and RS-79 showed tolerance to 11% NaCl. All the strains were able to grow in wider ranges of pH (4–10) and temperature (15–45°C) and had high tolerance to heavy metals. The inoculated bacterial strains significantly (
p
≤
0.05
) increased root and shoot length and dry biomass of acacia plants. One of the strains identified as P. fluorescens strain FB-49 was outstanding in enhancing DS tolerance compared to the single inoculants and comparable to consortia. Stress-tolerant PGPR could be used to enhance acacia DS tolerance after testing other phytobeneficial traits.
“…This assumption is valid in light of the results obtained in this study and similar results reported elsewhere, were Pseudomonas sp. showed a 97.9% lead (Pb), 93.5% cadmium (Cd) and 68% cupper (Cu) removal efficiency from contaminated industrial wastewater [23].…”
The threat of heavy metals pollution to public health and wildlife has led to an increased interest in developing systems that can remove or neutralize its toxic effects in industrial effluents and municipal wastewater. Tolerance to a range of heavy metal ions was determined for bacteria which had been isolated from wastewater collected from Makkah city, Saudi Arabia. Isolates were tolerant to cupper, cadmium, zinc, and cobalt although the levels of tolerance to the different concentrations of metal ions were specific for each isolate. One isolate was able to tolerate all four metal ions tested; phenotypic and genotypic investigation revealed that isolate (S7) resembled similarities with Pseudomonas aeruginosa. The results of this study showed the potential applicability of the isolated heavy metal-tolerant strain Pseudomonas aeruginosa (S7) in the treatment of heavy metal containing solutions. Further studies on the genomic structure of isolate (S7) are required to investigate its capabilities to remove/reduce heavy metals in contaminated microcosms. Methods Isolation of bacteria Water samples were taken from industrial wastewater ponds found in Makkah City, Saudi Arabia. The samples were collected in sterile screw-capped bottles containing 0.1% sodium thiosulfate to prevent bacterial oxidation. The collected samples were preserved in an ice box and transported to laboratory for direct bacteriological examination. Plate dilution method was employed for bacterial isolation [9] using LB agar supplemented with 0.5 mM of Cu(NO 3) 2 , CdCl 2 , Zn(NO 3) 2 and Co(NO 3) 2 respectively. An aliquot of 50 µl of each dilution was spread on the surface of LB agar plates; three replicates of each dilution were prepared. Inoculated plates were incubated at 30-35ºC for 24-48 h. The O.D. readings of each culture were taken at 0, 24, 48 and 72 h. The results were recorded based on three trials for each experiment. Identification tests Random collection of different colonies with various morphological characteristics was selected from LB agar plates. All unknown colonies were subjected to microscopic, biochemical and molecular identification as follows: Microscopic examination: Gram staining technique was carried out as described by Reddy et al. [9]. Biochemical characterization: Biochemical testes included: Oxidase, Catalase tests, Fermentation of Sugars, Urease test, IMVIC Test, aesculine hydrolysis, gelatin hydrolysis were carried out according to Reddy et al. [9]. J o u rn al of M ic ro b ia l & Bioc h e m ic a l Te chno lo g y
“…For example, bacillus flocculant has a good treatment effect on Cu 2+ and Pb 2+ . The removal rate of Cd 2+ by Pseudomonas can reach 93.5% [68]. The results show that the biosorption ability of the exopolysaccharides is much higher than that of the extracellular proteins in the extracellular polymers.…”
Section: Heavy Metal Wastewater Treatmentmentioning
In recent years, close attention has been paid to microbial flocculants because of their advantages, including safety to humans, environmental friendliness, and acceptable removal performances. In this review, the preparation methods of microbial flocculants were first reviewed. Then, the performances of bioflocculants in the removal of suspended solids, heavy metals, and other organic pollutants from various types of wastewater were described and commented, and the removal mechanisms, including adsorption bridging, charge neutralization, chemical reactions, and charge neutrality, were also discussed. The future research needs on microbial flocculants were also proposed. This review would lead to a better understanding of current status, challenges, and corresponding strategies on microbial flocculants and bioflocculation in wastewater treatment.
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