Removal of estrogenic activity of natural and synthetic hormones from a municipal wastewater: Efficiency of horseradish peroxidase and laccase from Trametes versicolor

Auriol, M; Filali-Meknassi, Youssef; Adams, Craig D.; Tyagi, Rajeshwar Dayal; Noguerol, Tania-Noelia; Piña, Benjamı́n

doi:10.1016/j.chemosphere.2007.06.064

Cited by 135 publications

(87 citation statements)

References 25 publications

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“…and Pycnoporus coccineus. Auriol et al (2008) degraded completely E1, EE2 and E2 after 1 h-treatment using 20 U/l and Suzuki et al (2003) transformed E2 and EE2 by 100% in 1 h by the laccase-HBT system. In addition, our research group (Lloret et al 2010) has recently used free Myceliophthora thermophila laccase for the elimination of these compounds, with a higher efficiency (total removal of E2 was attained in only 2 h, with less initial laccase activity and no mediator was used).…”

Section: Decolourisation Of a Model Synthetic Dye By Immobilised Laccasementioning

confidence: 96%

“…Unfortunately, we have not identified any reaction products by the HPLC protocol used, suggesting that a polymerisation reaction could have occurred or that the products reacted with either the laccase or the carrier. However, regarding the toxicity of estrogens treated by laccase, several reports have shown that the removal of estrogenic activity was correlated with the removal of the parent compound, indicating that the products of the degradation exhibited lower or no estrogenic activity (Auriol et al 2008). The estrogenic activity of the effluent of the continuous reactor was determined by the LYES assay.…”

Section: Decolourisation Of a Model Synthetic Dye By Immobilised Laccasementioning

confidence: 99%

“…This enzyme was reported to be a potential catalyst for the biodegradation of recalcitrant compounds: dyes, aromatic hydrocarbons compounds, olive oil mill wastewater and pulp delignification (Camarero et al 2007;Yamak et al 2009). In recent years, several research groups have explored the ability of free fungal laccases to remove a wide range of hormones: genistein, estrone, 17b-estradiol, estriol, and 17a-ethinylestradiol (Auriol et al 2008;Nicotra et al 2004;Tamagawa et al 2006;Lloret et al 2010). Crude and purified laccase from Trametes versicolor and Trametes villosa were used to degrade bisphenol A (Kim and Nicell 2006) and nonylphenol (Tsutsumi et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Immobilisation of laccase on Eupergit supports and its application for the removal of endocrine disrupting chemicals in a packed-bed reactor

et al. 2011

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Laccase from Myceliophthora thermophila was covalently immobilised on Eupergit C and Eupergit C 250L yielding specific activities of up to 17 and 80 U/g, respectively. Due to its superior activity, Eupergit C 250L was chosen for further research. The somewhat lower catalytic efficiency (based on the ratio between the turnover number and the Michaelis constant, k cat /K M ) of the immobilised enzyme in comparison with that of the free enzyme was balanced by its increased stability and broader operational window related to temperature and pH. The feasibility of the immobilised laccase was tested by using a packed bed reactor (PBR) operating in consecutive cycles for the removal of Acid Green 27 dye as model substrate. High degrees of elimination were achieved (88, 79, 69 and 57% in 4 consecutive cycles), while the levels of adsorption on the support varied from 18 to 6%, proving that dye removal took place mainly due to the action of the enzyme. Finally, a continuous PBR with the solid biocatalyst was applied for the treatment of a solution containing the following endocrine disrupting chemicals: estrone (E1), 17b-estradiol (E2) and 17a-ethinylestradiol (EE2). At steady-state operation, E1 was degraded by 65% and E2 and EE2 were removed up to 80% and only limited adsorption of these compounds on the support, between 12 and 22%, was detected. In addition, a 79% decrease in estrogenic activity was detected in the effluent of the enzymatic reactor while only 14% was attained by inactivated laccase.

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Section: Decolourisation Of a Model Synthetic Dye By Immobilised Laccasementioning

confidence: 96%

Section: Decolourisation Of a Model Synthetic Dye By Immobilised Laccasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immobilisation of laccase on Eupergit supports and its application for the removal of endocrine disrupting chemicals in a packed-bed reactor

et al. 2011

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“…A similar induction was noticed after phthalates, BPA and NP addition to a T. versicolor culture: the secreted enzymatic pattern further degraded the molecules tested (Kim et al 2008). Cheong and collaborators (2006) (Auriol et al 2008, Kim and Nicell 2006a, Lloret et al 2010, Lloret et al 2012a, Lloret et al 2013, Murugesan et al 2010, Songulashvili et al 2012, Torres-Duarte et al 2012, Tran et al 2013). Transformation rates of many compounds were not always coherent among evidences present in literature.…”

Section: Fungal Laccasesmentioning

confidence: 99%

“…These data acquire great importance if referred to municipal wastewaters. Oxybenzone removal required 2 hours (Garcia et al 2011), whereas 20,000 U/l completely removed estrogens in a wastewater sample within a 1 hours (Auriol et al 2008). The half-life was lower than 4 hours for 10 up to 18 micropollutants, and most of these occurred in the first minutes of the treatment (Spina et al 2015).…”

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confidence: 99%