Removal of cadmium ions during stationary growth phase by an extremely cadmium‐resistant strain of <i>Stenotrophomonas</i> sp.

Chien, Chih-Ching; Hung, Chun‐Wei; Han, Chu-Ting

doi:10.1897/06-280r.1

Cited by 30 publications

(12 citation statements)

References 35 publications

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“…RRLJSMAR showed resistance to Cu concentrations. Another study, conducted by Chien et al (2007), evaluated the bacterial diversity in soil in order to determine bacterial response to media amended with Cd, Cr, Ni, Zn, Pb, and Cu. Stenotrophomonas sp., isolated from a site contaminated with high concentrations of Cd (3 mg.kg ), was able to grow on media containing Cd concentrations of up to 4 mM, and was also able to remove up to 80% dissolved ions upon completion of the stationary growth phase.…”

Section: Znmentioning

confidence: 99%

“…Stenotrophomonas sp., isolated from a site contaminated with high concentrations of Cd (3 mg.kg ), was able to grow on media containing Cd concentrations of up to 4 mM, and was also able to remove up to 80% dissolved ions upon completion of the stationary growth phase. In addition it was also able to resist other metals, such as, Cu, Cr, Ni, Pb and Zn, at levels of more than 2 mM Chien et al (2007).…”

Section: Znmentioning

confidence: 99%

“…This genetic variation can then be visualised with phylogenetic trees (Martin, 2002). Chien et al (2007) studied the microbial diversity in soil contaminated with effluent from a chemical industrial factory, using 16S rDNA. The organisms isolated were Polyangium spp., Sphingomonas spp., Variovorax spp., Hafina spp., Clos tridia, Acidobacteria, the enterics and some uncultured strains.…”

Section: Introductionmentioning

confidence: 99%

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Identification of metal-tolerant organisms isolated from the Plankenburg River, Western Cape, South Africa

Jackson

Paulse

Odendaal

et al. 2012

WSA

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The ability of biofilms to resist pollutants makes them advantageous for use in bioremediation. The objective of this investigation was to isolate metal-tolerant micro-organisms from a site along the Plankenburg River. Microbial biofilms cultivated in multi-channelled flow cells were exposed to varying concentrations of aluminium (Al), iron (Fe), copper (Cu), manganese (Mn), nickel (Ni) and zinc (Zn), stained with the BacLight TM viability probe, visualised using epifluorescence microscopy and analysed using ScionImage. Exposure to the highest Al, Fe, Cu and Mn concentrations increased the percentages of dead cells. A difference in live and dead cells after exposure to varying Zn and Ni concentrations was not evident. When exposed to the lowest concentrations, no notable difference could be detected in comparison with the untreated control. Possible metal-tolerant micro-organisms were identified from the exposed flow cells using polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) sequencing, followed by ClustalX alignment and phylogenetic analysis. Phylogenetic analysis identified a variety of organisms, including Bacillus sp., Pseudomonas sp., Delftia tsuruhatensis strain A90, Kocuria kristinae strain 6J-5b, Comamonas testosteroni WDL7, Stenotrophomonas maltophilia strain 776, Staphylococcus sp. MOLA:313, Micrococcus sp. TPR14, Sphingomonas sp. 8b-1 and Microbacterium sp. PAO-12. Two major clusters could be distinguished based on their Gram-reactions.

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Section: Znmentioning

confidence: 99%

Section: Znmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of metal-tolerant organisms isolated from the Plankenburg River, Western Cape, South Africa

Jackson

Paulse

Odendaal

et al. 2012

WSA

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“…It has, for instance, been isolated from the rhizosphere of various plants and animals (14,27,37). Due to its tolerance against cadmium and its ability to degrade xenobiotic compounds, it has been proposed for remediation of contaminated ground (9,39). Increasingly, it is being isolated from immunosuppressed individuals and intensive care and cystic fibrosis (CF) patients and has been shown to be resistant to many antimicrobial agents (16,17,69).…”

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confidence: 99%

A Stenotrophomonas maltophilia Multilocus Sequence Typing Scheme for Inferring Population Structure

2009

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Stenotrophomonas maltophilia is an opportunistic, highly resistant, and ubiquitous pathogen. Strains have been assigned to genogroups using amplified fragment length polymorphism. Hence, isolates of environmental and clinical origin predominate in different groups. A multilocus sequence typing (MLST) scheme was developed using a highly diverse selection of 70 strains of various ecological origins from seven countries on all continents including strains of the 10 previously defined genogroups. Sequence data were assigned to 54 sequence types (ST) based on seven loci. Indices of association for all isolates and clinical isolates of 2.498 and 2.562 indicated a significant linkage disequilibrium, as well as high congruence of tree topologies from different loci. Potential recombination events were detected in one-sixth of all ST. Calculation of the mean divergence between and within predicted clusters confirmed previously defined groups and revealed five additional groups. Consideration of the different ecological origins showed that 18 out of 31 respiratory tract isolates, including 12 out of 19 isolates from cystic fibrosis (CF) patients, belonged to genogroup 6. In contrast, 16 invasive strains isolated from blood cultures were distributed among nine different genogroups. Three genogroups contained isolates of strictly environmental origin that also featured high sequence distances to other genogroups, including the S. maltophilia type strain. On the basis of this MLST scheme, isolates can be assigned to the genogroups of this species in order to further scrutinize the population structure of this species and to unravel the uneven distribution of environmental and clinical isolates obtained from infected, colonized, or CF patients.Stenotrophomonas maltophilia is ubiquitous in nature. It has, for instance, been isolated from the rhizosphere of various plants and animals (14, 27, 37). Due to its tolerance against cadmium and its ability to degrade xenobiotic compounds, it has been proposed for remediation of contaminated ground (9, 39). Increasingly, it is being isolated from immunosuppressed individuals and intensive care and cystic fibrosis (CF) patients and has been shown to be resistant to many antimicrobial agents (16,17,69). However, the role of this opportunistic pathogen as an innocent bystander or causative agent often remains unclear (30), and little is known about its virulence factors (20,33).Recently, novel Stenotrophomonas species were described: Stenotrophomonas nitritireducens sp. nov. (24), Stenotrophomonas acidaminiphila (3), Stenotrophomonas rhizophila (73), and Stenotrophomonas africana sp. nov. (21). However, the latter is a synonym of S. maltophilia (10).Using amplified fragment length polymorphism (AFLP) fingerprinting and DNA-DNA hybridizations, remarkable diversity has been shown to exist among S. maltophilia isolates recovered from both the environment and human clinical samples. This species can be subdivided into 10 AFLP genomic groups (35) that comprise to various extents both clinical ...

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“…The growth of Escherichia coli could be completely inhibited in the presence of 0.5 mM Cd 2þ [6]. Tellurite is toxic to planktonic microorganisms at concentrations as low as 1 mg/ml, although cells in biofilms are more tolerant of it [2,3,7,8]. Microorganisms that thrive in environments contaminated with industrial pollutants often evolve different strategies to counteract these toxic ions.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of an environmental cadmium‐ and tellurite‐resistant Pseudomonas strain

Chien

Jiang

Tsai

2011

Enviro Toxic and Chemistry

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A Pseudomonas strain (TeU), resistant to tellurite (TeO(2)(3)(-) and cadmium (Cd(2+)) ions, was isolated from heavy-metal-contaminated sediments by enrichment. Black precipitates, presumably the product of the reduction of tellurite, such as tellurium, occurred in cultures of the isolate after growth in medium containing tellurite. Quantitative determination of the TeO(2)(3)(-) concentration in the liquid culture demonstrated a decreased concentration of tellurite (to less than 100 µM) from initial concentrations of approximately 1,000 µM within 24 h of growth. Strain TeU was resistant to TeO(2)(3)(-) and Cd(2+) concentrations as high as 2,000 µM and 500 µM, respectively. Transposon mutagenesis of strain TeU resulted in mutants exhibiting Cd(2+) sensitivity (Strain BU21) and one with decreased ability to reduce tellurite (strain AU08). Strain BU21 was less tolerant to Cd(2+) (100 µM) compared with the wild-type strain TeU (500 µM) but was still able to reduce tellurite to 80% of that of strain TeU. Although strain AU08 possesses the ability for Cd(2+) resistance, it reduced less than 20% of the initial concentrations of tellurite compared with strain TeU. Genes encoding an HflKC complex and a putative metallopeptidase were associated with the bacterium's capacity for tellurite reduction and Cd resistance, respectively. The ability to reduce tellurite therefore may not be necessary for this bacterium's heavy metal and metalloid tellurite resisting ability.

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Removal of cadmium ions during stationary growth phase by an extremely cadmium‐resistant strain of Stenotrophomonas sp.

Cited by 30 publications

References 35 publications

Identification of metal-tolerant organisms isolated from the Plankenburg River, Western Cape, South Africa

Identification of metal-tolerant organisms isolated from the Plankenburg River, Western Cape, South Africa

A Stenotrophomonas maltophilia Multilocus Sequence Typing Scheme for Inferring Population Structure

Isolation and characterization of an environmental cadmium‐ and tellurite‐resistant Pseudomonas strain

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