Removal of B. cereus cereulide toxin from monoclonal antibody bioprocess feed via two-step Protein A affinity and multimodal chromatography

Wetterhall, Magnus; Grönberg, Anna; Grönlund, Sara; Björkman, Tomas; Sandberg, Lena; Musunuri, Sravani; Chaloupka, Katherine; Gammell, Patrick

doi:10.1016/j.jchromb.2019.04.018

Cited by 5 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we identified an isolate that carried the ces gene and among the enterotoxin genes we detected only the entS gene. Based on genetic profile, we supposed that the infection due to the two isolates belonging to profile 1 (ST4; ST33) could be associated to a worst-case scenario [ 73 , 74 ], since they harboured all the genes investigated. The genetic profiles 2 and 5 represented the largest group, which included a total of 39 and 27 isolates, respectively.…”

Section: Discussionmentioning

confidence: 99%

High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

Bianco

Normanno

Capozzi

et al. 2023

Foods

View full text Add to dashboard Cite

The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato (s.l.). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection revealed that 90% and 61% of the isolates harboured the nheABC and the hblCDA gene cluster, respectively. Ninety-four percent of the isolates harboured the enterotoxin genes entS and entFM; 8% of the isolates possessed the ces gene. Thirty-eight different genetic profiles were identified, suggesting a high genetic diversity. Our study clearly shows the widespread diffusion of potentially toxigenic isolates of B. cereus s.l. in milk and cheeses in the Apulia region highlighting the need to adopt GMP and HACCP procedures along every step of the milk and cheese production chain in order to reduce the public health risk linked to the consumption of foods contaminated by B. cereus s.l.

show abstract

Section: Discussionmentioning

confidence: 99%

High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

Bianco

Normanno

Capozzi

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

“…All these data seem to suggest that emetic toxin gene is rare among B. cereus s.l. strains, but an infection supported by this specific toxin is considered to be the worst-case scenario (Wetterhall et al , 2019 ). Generally, B. cereus s.l.…”

Section: Discussionmentioning

confidence: 99%

Multi-locus sequence typing and virulence profile in <em>Bacillus cereus sensu</em> lato strains isolated from dairy products

et al. 2021

View full text Add to dashboard Cite

Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products.

show abstract

Investigation of alkaline effects on Protein A affinity ligands and resins using high resolution mass spectrometry

Wetterhall

Ander

Björkman

et al. 2021

Journal of Chromatography B

View full text Add to dashboard Cite

Removal of B. cereus cereulide toxin from monoclonal antibody bioprocess feed via two-step Protein A affinity and multimodal chromatography

Cited by 5 publications

References 27 publications

High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

Multi-locus sequence typing and virulence profile in <em>Bacillus cereus sensu</em> lato strains isolated from dairy products

Investigation of alkaline effects on Protein A affinity ligands and resins using high resolution mass spectrometry

Contact Info

Product

Resources

About