Removal of aqueous phenol and phenol derivatives by immobilized potato polyphenol oxidase

Lončar, Nikola; Božić, Nataša; Andjelkovic, Ivan; Milovanović, Aleksandra; Dojnov, Biljana; Vujčić, Miroslava; Roglić, Goran; Vujčić, Zoran

doi:10.2298/jsc100619046l

Cited by 25 publications

(18 citation statements)

References 18 publications

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“…Da bi se prevazišli ovi problemi, upotreba enzima ukljuĉenih u razgradnju boje, umjesto ĉitavih mikrobnih kultura privlaĉi pažnju istraživaĉa. Prednost korišćenja enzima umjesto kultura se zasniva na kraćem tretmanu, radu sa visokim i niskim koncentracijama supstrata, odsustvu lag perioda neophodnog za formiranje biomase, smanjenju koliĉine formiranog mulja i lakoći kontrole procesa (Akhtar i Husain 2006;Lonĉar et al, 2011).…”

Section: Biološke Metode Uklanjanja Ksenobiotikaunclassified

“…Celulozna zrna granulacije (90 µm) obogaćena sa razliĉitom koliĉinom Ti (0,25, 0,7 i 1,0 %, w/w), kao i Celuloza M, takoĊe modifikovana titanijumom (Meng et al, 2002) Levy et al, 2003;Lonĉar et al, 2011;Tong et al, 1997).…”

Section: Imobilizacija Ppounclassified

“…Nakon osam uzastopnih ciklusa, od kojih je svaki trajao po 5h, efikasnost uklanjanja p-CP pomenutim biokatalizatorom pokazuje trend pada na 55%, slika 15 (Lonĉar et al, 2011). Zapaženo je formiranje i akumulacija tamnih precipitata na imobilizatu, što prividno smanjuje aktivnost imobilizata, jer se akumulacijom precipitata na imobilizatu onemogućava kontakt enzima sa supstratom.…”

Section: 123) Eksperimentalni Podaci Pokazuju Da Najbolje Rezultunclassified

“…Epihlorhidrinska aktivacija DEAE celuloze je praćena uvoĊenjem iminodiacetatnih grupa. Joni bakra se koordinativno vezuju za iminodiacetatne grupe, a tri molekula vode se u toku imobilizacije zamijenjuju koordinovanjem sa atomima N, O ili S u boĉnim aminokiselinskim ostacima na površini proteina (Lonĉar i Vujĉić, 2011). ak ̶ aminokiselinski ostatak na površini proteina.…”

Section: Sinteza Novog Nosača Sa Pipcima I Njegova Primjena Za Imobilunclassified

“…Vezivanje enzima (imobilizacija) se postiže vezivanjem za bakar N, O i S atoma boĉnih aminokiselinskih ostataka na površini enzima (Lonĉar i Vujĉić, 2011). Ovako dobijen biokatalizator je testiran za uklanjanje halogenfenola u šaržnom reaktoru i pokazao je izuzetan potencijal uklanivši skoro u potpunosti p-CP i p-BP iz sintetiĉke otpadne vode.…”

Section: Sinteza Novog Nosača Sa Pipcima I Njegova Primjena Za Imobilunclassified

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Removal of phenols and dyes from wastewater using native and recombinant oxidative enzymes

Lončar¹

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Section: Biološke Metode Uklanjanja Ksenobiotikaunclassified

Section: Imobilizacija Ppounclassified

Section: 123) Eksperimentalni Podaci Pokazuju Da Najbolje Rezultunclassified

Section: Sinteza Novog Nosača Sa Pipcima I Njegova Primjena Za Imobilunclassified

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Removal of phenols and dyes from wastewater using native and recombinant oxidative enzymes

Lončar¹

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Trametes versicolor laccase immobilized poly(glycidyl methacrylate) based cryogels for phenol degradation from aqueous media

Doğan

Bayram

Uzun

et al. 2015

J of Applied Polymer Sci

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This study aims removal of phenols in wastewater by enzymatic oxidation method. In this study, Trametes versicolor laccase was covalently immobilized onto a cryogel matrix by the nucleophilic attack of amino groups of laccase to epoxy groups of matrix. Glycidyl methacrylate was chosen as functional monomer to prepare poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [p(HEMA-co-GMA)] cryogels. The enzyme immobilized matrix was characterized by FTIR, SEM, and swelling tests. The effect of pH, reaction time, temperature, substrate concentration, enzyme concentration, and storage period on immobilized enzyme activity was determined and compared with those of free enzyme. The model substrate was 2,2 0 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Lineweaver-Burk plots were used to calculate K m and V m values. K m values were 165.1 and 156.0 mM while V m values were 55.2 mM min 21 and 1.57 mM min 21 for free and immobilized laccase, respectively. Immobilized enzyme was determined to retain 82.5% and 72.0% of the original activity, respectively, after 6 consecutive use and storage period of 4 weeks. The free enzyme retained only 24.0% of its original activity following the same storage period. Lastly, decomposition products resulting from enzymatic oxidation of a model phenolic compound (3,5-dinitrosalicylic acid) in aqueous solution were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

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Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field

Xia

Feng

Liu

et al. 2021

Engineering in Life Sciences

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Enzymatic degradation of emerging contaminants has gained great interest for the past few years. However, free enzyme often incurs high costs in practice. The immobilized laccase on the polyethylenimine (PEI)‐functionalized magnetic nanoparticles (Fe3O4–NH2–PEI–laccase) was fabricated to efficiently degrade phenolic compounds continuously in a newly fixed bed reactor under a high‐gradient magnetic field. The degradation rate of continuous treatment in the bed after 18 h was 2.38 times as high as that of batch treatment after six successive operations with the same treatment duration. Under the optimal conditions of volume fraction of nickel wires mesh, flow rate of phenol solution, phenol concentration, and Fe3O4–NH2–PEI–laccase amount, the degradation rate of phenol kept over 70.30% in 48 h continuous treatment. The fixed bed reactor filled with Fe3O4–NH2–PEI–laccase provided a promising avenue for the continuous biodegradation of phenolic compounds for industrial wastewater in practice.

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Removal of aqueous phenol and phenol derivatives by immobilized potato polyphenol oxidase

Cited by 25 publications

References 18 publications

Removal of phenols and dyes from wastewater using native and recombinant oxidative enzymes

Removal of phenols and dyes from wastewater using native and recombinant oxidative enzymes

Trametes versicolor laccase immobilized poly(glycidyl methacrylate) based cryogels for phenol degradation from aqueous media

Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field

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