Removal of antibiotic resistance genes in an algal-based wastewater treatment system employing Galdieria sulphuraria: A comparative study

Cheng, Xiaoli; Delanka-Pedige, H.M.K.; Munasinghe-Arachchige, S.P.; Abeysiriwardana-Arachchige, I.S.A.; Smith, Geoffrey B.; Nirmalakhandan, Nagamany; Zhang, Yanyan

doi:10.1016/j.scitotenv.2019.134435

Cited by 62 publications

(19 citation statements)

References 60 publications

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“…As for bla TEM , the highest LRV of the gene was 1.869. This moderate reduction was also reported in a comparative study where the relative abundance of the gene was lower in algal treatment plant effluent compared with both primary and secondary (activated sludge) treatment systems operating in parallel [ 23 ]. However, contrary to this, increased relative abundance of the gene was reported in the effluents of full-scale wastewater treatment plants [ 28 ].…”

Section: Discussionsupporting

confidence: 61%

“…Regardless, at the end of the treatment, the CM2 culture recorded the highest ( p < 0.05) reduction of the gene (1.45 LRV) than all other cultures, including KL10. This magnitude of reduction was still higher than the 1.14 LRV achieved by artificially-lighted AWWT [ 11 ] and increased relative abundance observed in an acidophilic microalga-based wastewater treatment system [ 23 ].…”

Section: Discussionmentioning

confidence: 93%

“…The final temperature of the cultures did not differ significantly from one another, suggesting that temperature could not have played any significant role in any perceived differences in the performances of the cultures. However, previous studies have reported the temperature of the microalgal culture medium to be a key player in the overall performance of the system [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

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Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System

et al. 2022

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Microalgae-based wastewater treatment systems (AWWTS) have recently shown promise in the mitigation of antibiotic resistance genes (ARGs) from municipal wastewater (MWW). However, due to the large number of ARGs that exist in MWW, the use of indirect conventional water quality parameters to monitor ARGs reduction in wastewater would make the process less burdensome and economically affordable. In order to establish a robust relationship between the ARGs and water quality parameters, the current study employed different microalgae strains in monoculture (CM2, KL10) and multi-species combinations (CK and WW) for the MWW treatment under outdoor environmental conditions. The studied genes were quantified in the MWW influents and effluents using real-time PCR. All the cultures substantially improved the physicochemical qualities of the MWW. Out of the 14 genes analyzed in this study, tetO, tetW, tetX and ermB were decreased beyond detection within the first 4 days of treatment in all the cultures. Other genes, including blaCTX, sul1, cmlA, aadA, int1 and uidA were also decreased beyond a 2 log reduction value (LRV). The mobile genetic element, int1, correlated positively with most of the ARGs, especially sul1 (r ≤ 0.99, p < 0.01) and aadA (r ≤ 0.97, p < 0.01). Similarly, the Escherichia coli indicator gene, uidA, correlated positively with the studied genes, especially with aadA, blaCTX, blaTEM and cmlA (r ≤ 0.99 for each, p < 0.01). Some of the studied genes also correlated positively with total dissolved solids (TDS) (r ≤ 0.98, p < 0.01), and/or negatively with total suspended solids (TSS) (r ≤ −0.98, p < 0.01) and pH (r ≤ −0.98, p < 0.01). Among the tested cultures, both monocultures, i.e., KL10 and CM2 were found to be more consistent in gene suppression than their multi-species counterparts. The findings revealed water quality parameters such as TDS, TSS and E. coli as reliable proxies for ARGs mitigation in AWWTS and further highlight the superiority of monocultures over multi-species cultures in terms of gene suppression from the MWW stream.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System

et al. 2022

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“…Interestingly, a molecular imprinting-based biosensor may help in diagnosing and monitoring phage-mediated HGT (Ertürk and Lood, 2018). Furthermore, the latest research also shows that an algal-based wastewater treatment system can reduce the frequency of phage-mediated HGT (Cheng et al, 2020).…”

Section: Phage-mediated Horizontal Gene Transfermentioning

confidence: 99%

Bacteriophages in water pollution control: Advantages and limitations

Liu

Sun

et al. 2020

Front. Environ. Sci. Eng.

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“…Until now, different technologies can remove ARBs and ARGs from wastewater effluents. These can go from membrane bioreactor treatments (Kappell et al, 2018;Riquelme Breazeal et al, 2013) to coagulation (Li et al, 2017) or algal-based wastewater treatment systems (Cheng et al, 2020). The aforementioned methods however employ non-renewable materials or require consistent variations on the WWTP operation processes.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance genes and mobile genetic elements removal from treated wastewater by sewage-sludge biochar and iron-oxide coated sand

Calderón-Franco

Apoorva

Medema

et al. 2020

Preprint

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Disinfection of treated wastewater in wastewater treatment plants (WWTPs) is used to minimize emission of coliforms, pathogens, and antibiotic resistant bacteria (ARB) in the environment. However, the fate of free-floating extracellular DNA (eDNA) that do carry antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) is overlooked. Water technologies are central to urban and industrial ecology for sanitation and resource recovery. Biochar produced by pyrolysis of sewage sludge and iron-oxide-coated sands recovered as by-product of drinking water treatment were tested as adsorbents to remove ARGs and MGEs from WWTP effluent. DNA adsorption properties and materials applicability were studied in batch and up-flow column systems at bench scale. Breakthrough curves were measured with ultrapure water and treated wastewater at initial DNA concentrations of 0.1-0.5 mg mL-1 and flow rates of 0.1-0.5 mL min-1. Batch tests with treated wastewater indicated that the adsorption profiles of biochar and iron-oxide coated sand followed a Freundlich isotherm, suggesting a multilayer adsorption of nucleic acids. Sewage-sludge biochar exhibited higher DNA adsorption capacity (1 mg g-1) and longer saturation breakthrough times (4 to 10 times) than iron-oxide coated sand (0.2 mg g-1). The removal of a set of representative ARGs and MGEs was measured by qPCR comparing the inlet and outlet of the plug-flow column fed with treated wastewater. ARGs and MGEs present as free-floating eDNA were adsorbed by sewage-sludge biochar at 85% and iron-oxide coated sand at 54%. From the environmental DNA consisting of the free-floating extracellular DNA plus the intracellular DNA of the cells present in the effluent water, 97% (sewage-sludge biochar) and 66% (iron-oxide coated sand) of the tested genes present were removed. Sewage-sludge biochar displayed interesting properties to minimize the spread of antimicrobial resistances to the aquatic environment while strengthening the role of WWTPs as resource recovery factories.Graphical abstractHighlightsSewage-sludge biochar and iron oxide coated sands were tested to adsorb DNA and cells.Biochar removed 97% of genes tested from environmental DNA of unfiltered effluent.85% of ARGs and MGEs of free-floating extracellular DNA were retained by biochar.Biochar is a WWTP by-product that can be re-used for public health sanitation.

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Removal of antibiotic resistance genes in an algal-based wastewater treatment system employing Galdieria sulphuraria: A comparative study

Cited by 62 publications

References 60 publications

Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System

Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System

Bacteriophages in water pollution control: Advantages and limitations

Antibiotic resistance genes and mobile genetic elements removal from treated wastewater by sewage-sludge biochar and iron-oxide coated sand

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