When shared between remote locations, entanglement opens up fundamentally new capabilities for science and technology [1,2]. Envisioned quantum networks distribute entanglement between their remote matter-based quantum nodes, in which it is stored, processed and used [1]. Pioneering experiments have shown how photons can distribute entanglement between single ions or single atoms a few ten meters apart [3,4] and between two nitrogen-vacancy centres 1 km apart [5]. Here we report on the observation of entanglement between matter (a trapped ion) and light (a photon) over 50 km of optical fibre: a practical distance to start building large-scale quantum networks. Our methods include an efficient source of light-matter entanglement via cavity-QED techniques and a quantum photon converter to the 1550 nm telecom C band. Our methods provide a direct path to entangling remote registers of quantum-logic capable trapped-ion qubits [6][7][8], and the optical atomic clock transitions that they contain [9,10], spaced by hundreds of kilometers.Our network node consists of a 40 Ca + ion in a radiofrequency linear Paul trap with an optical cavity that enhances photon collection on the 854 nm electronic dipole transition. (Figure 1). A Raman laser pulse at 393 nm triggers emission, by the ion, of a photon into the cavity via a bichromatic cavity-mediated Raman transition (CMRT) [11]. Two indistinguishable processes are driven in the CMRT, each leading to the generation of a cavity photon and resulting in entanglement between photon polarisation and the electronic qubit state of the ion of the form 1/ √ 2 (|D J=5/2, mj =−5/2 , V + |D J=5/2, mj =−3/2 , H ), with horizontal (H) and vertical (V ) photon polarisation and two metastable Zeeman states of the ion (D J, mj ) [12]. The total probability of obtaining an on-demand free-space photon out of the ion vacuum chamber (entangled with the ion) is 0.5 ±0.1 [12].While the ∼ 3 dB/km losses suffered by 854 nm photons through state-of-the-art optical fibre allows for few km internode distances, transmission over 50 km would be 10 −15 . 854 nm photons are also frequencyincompatible with other examples of quantum matter, preventing the realisation of ion-hybrid quantum systems over any distance. Single photon frequency conversion to the telecom C band (1550 nm) offers a powerful general solution: this wavelength suffers the minimum fibre transmission losses (∼ 0.18 dB/km) and is therefore an ideal choice for a standard interfacing wavelength for quantum networking. Photons from solid-state memories [14], cold gas memories [15,16], quantum dots and nitrogen-vacancy centres [17] have been converted to telecom wavelengths. Frequency conversion of photons from ions has recently been performed, including to the telecom C band (without entanglement) [18], to the telecom * ben.lanyon@uibk.ac.at, † These authors contributed equally O band with entanglement over 80 m [19] and directly to an atomic Rubidium line at 780 nm [20].We inject single-mode fibre-coupled photons from the ion into a polarisat...