Remote near infrared identification of pathogens with multiplexed nanosensors

Nißler, Robert; Bader, Oliver; Dohmen, Maria; Walter, Sebastian; Noll, Christine; Selvaggio, Gabriele; Groß, Uwe; Kruss, Sebastian

doi:10.1038/s41467-020-19718-5

Cited by 113 publications

(128 citation statements)

References 95 publications

(103 reference statements)

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“…However, given the overall low signal strength when measuring with this device, the TCSPC values should be regarded as the gold-standard. NIR Imaging at Stand-Off Detection Setup: Our NIR custom-made stand-off detection setup [18] consists of a NIR InGaAs camera (XEVA, Xenics), a Kowa objective (f = 25 mm/F1.4), and a white light source (UHPLCC-01, UHP-LED-white, Prizmatix). The latter is equipped with a 700 nm short pass filter (FESH0700, Thorlabs) for excitation.…”

Section: Exfoliation Of Egyptian Blue (Eb) Han Blue (Hb) and Han Purmentioning

confidence: 99%

“…A novel class of 2D materials are (phyllo)silicates such as Egyptian Blue (CaCuSi4O10, EB), which is as (exfoliated) nanosheet a novel fluorophore for photonics [17,18] . This calcium copper tetrasilicate is regarded as the most ancient artificial pigment made by mankind and dates back to Ancient Egypt (≈ 2500 BC), where it was employed in artwork [19] .…”

Section: Introductionmentioning

confidence: 99%

“…indocyanine green (ICG)), nanomaterials such as InAs quantum dots, lanthanide-doped nanoparticles, or semiconducting single-walled carbon nanotubes (SWCNTs) offer an enhanced photostability and a handle for chemical functionalization [43][44][45][46][47][48] . Especially for SWCNTs chemical concepts have been developed to conjugate a broad range of biomolecules, which is required for molecular recognition [18,[49][50][51][52][53] . Nevertheless, there is a large demand for novel NIR fluorophores with better or complementary properties.…”

Section: Introductionmentioning

confidence: 99%

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Exfoliation and Optical Properties of Near-Infrared Fluorescent Silicate Nanosheets

Selvaggio¹,

Weitzel²,

Oleksiievets³

et al. 2021

Preprint

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<div><div><div><p>The silicates Egyptian Blue (CaCuSi4O10, EB), Han Blue (BaCuSi4O10, HB) and Han Purple (BaCuSi2O6, HP) emit in bulk bright and stable fluorescence in the near-infrared (NIR), which is of high interest for (bio)photonics due to minimal scattering, absorption and phototoxicity in this spectral range. So far the optical properties of nanosheets (NS) of these silicates are poorly understood. Here, we exfoliate them into nanosheets and report their physicochemical properties. The approach uses ball milling followed by tip sonication and centrifugation steps to exfoliate the silicates into NS with a lateral size ≈ 16-27 nm and thickness ≈ 1-4 nm. They emit at ≈ 927 nm (EB-NS), 953 nm (HB-NS) and 924 nm (HP-NS) and single NS can be resolved in the NIR. Fluorescence lifetimes decrease from ≈ 30-100 μs (bulk) to 17 μs (EB- NS), 8 μs (HB-NS) and 7 μs (HP-NS). NS of different composition/size can be imaged by fluorescence lifetime imaging, which enables lifetime-encoded multicolor imaging both on the microscopic and the macroscopic scale. Finally, remote imaging through tissue phantoms reveals the potential for bioimaging. In summary, we report a procedure to gain NIR fluorescent silicate nanosheets, characterize their photophysical properties and show their potential for NIR photonics.</p></div></div></div>

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Section: Exfoliation Of Egyptian Blue (Eb) Han Blue (Hb) and Han Purmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exfoliation and Optical Properties of Near-Infrared Fluorescent Silicate Nanosheets

Selvaggio¹,

Weitzel²,

Oleksiievets³

et al. 2021

Preprint

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“…[28] A different approach for SWCNT-based molecular recognition relies on biomolercular binding elements such as antibodies, [55][56][57] aptamers, [58,59] and other binding partners. [60][61][62][63][64][65][66][67][68][69][70] Aptamers are short, single stranded nucleic acids which are selected for their specific target using the SELEX procedure (systematic evolution of ligands by exponential enrichment). [71] Often, their high affinity to the targets results from a conformational effect as they may fold around the target upon binding.…”

Section: Introductionmentioning

confidence: 99%

Optical Nanosensors for Real-time Feedback on Insulin Secretion by β-Cells

Ehrlich

Hendler‐Neumark

Wulf

et al. 2021

Preprint

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Quantification of insulin is essential for diabetes research in general, and for the study of pancreatic β-cell function in particular. Herein, fluorescent single-walled carbon nanotubes (SWCNT) are used for the recognition and real-time quantification of insulin. Two approaches for rendering the SWCNT sensors for insulin are compared, using surface functionalization with either a natural insulin aptamer with known affinity to insulin, or a synthetic PEGylated-lipid (C16-PEG(2000Da)-Ceramide), both of which show a modulation of the emitted fluorescence in response to insulin. Although the PEGylated-lipid has no prior affinity to insulin, the response of C16-PEG(2000Da)-Ceramide-SWCNTs to insulin is more stable and reproducible compared to the insulin aptamer-SWCNTs. The C16-PEG(2000Da)-Ceramide-SWCNTs optical response is excitation-wavelength dependent, where resonant excitation leads to a larger fluorescence decrease in response to insulin. The SWCNT sensors successfully detect insulin secreted by β-cells within the complex environment of the conditioned media. The insulin is quantified by comparing the SWCNTs fluorescence response to a standard calibration curve, and the results are found to be in agreement with an enzyme-linked immunosorbent assay (ELISA). This novel analytical tool for real time quantification of insulin secreted by β-cells provides new opportunities for rapid assessment of β-cell function, with the ability to push forward many aspects of diabetes research.

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“…DNA [10][11][12][13] , DNA-peptide conjugates 14,15 , lipids 16,17 , nanobodies 18 or proteins 19,20 have been successfully used to engineer selective surface chemistry. These approaches enabled a range of sensors for important biomolecules such as reactive oxygen species 15,21,22 , metabolites 23 , sugars 24,25 , neurotransmitters 26,27 , proteins 17,28 and lipids 29 . However, how the molecular interaction with a target molecule affects SWCNT photophysics and decay pathways is still not understood.…”

Section: Introductionmentioning

confidence: 99%

Quantum Defects in Fluorescent Carbon Nanotubes for Sensing and Mechanistic Studies

Spreinat¹,

Dohmen²,

Lüttgens³

et al. 2021

Preprint

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<div><div><div><p>Single wall carbon nanotubes (SWCNT) fluoresce in the near infrared (NIR) and have been assembled with biopolymers such as DNA to form highly sensitive molecular sensors. They change their fluorescence when they interact with analytes. Despite the progress in engineering of these sensors the underlying mechanisms are still not understood. Here, we identify processes and rate constants that explain the photophysical signal transduction by exploiting sp3 quantum defects in the sp2 carbon lattice of SWCNTs. As a model system we use ssDNA coated (6,5)-SWCNTs, which increase their NIR emission (E11, 990 nm) up to + 250 % in response to the important neurotransmitter dopamine. In contrast, SWCNTs coated with DNA but with a low number of NO2-Aryl sp3 quantum defects decrease both their E11 (-35%) and defect related E11* emission (- 50%) at 1130 nm. Consequently, the interaction with the analyte does not change the radiative exciton decay pathway alone. Furthermore, the fluorescence response of pristine SWCNTs increases with SWCNT length, suggesting that exciton diffusion is affected. The quantum yield of pristine (6,5)-SWCNTs increases in response to the analyte from 0.6 % to 1.3 % and points to a change in non-radiative rate constants. These experimental results are explained by a Monte Carlo simulation of exciton diffusion, which supports a change of two non-radiative decay pathways together with an increase of exciton diffusion (3 rate constant model). The combination of such SWCNTs with defects and without defects enables the assembly of ratiometric sensors with opposing responses at different wavelengths. In summary, we demonstrate how perturbation of a system with quantum defects reveals the photophysical mechanism and reverses optical responses.</p></div></div></div>

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Remote near infrared identification of pathogens with multiplexed nanosensors

Cited by 113 publications

References 95 publications

Exfoliation and Optical Properties of Near-Infrared Fluorescent Silicate Nanosheets

Exfoliation and Optical Properties of Near-Infrared Fluorescent Silicate Nanosheets

Optical Nanosensors for Real-time Feedback on Insulin Secretion by β-Cells

Quantum Defects in Fluorescent Carbon Nanotubes for Sensing and Mechanistic Studies

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