Remodeling the tumor immune microenvironment via siRNA therapy for precision cancer treatment

Jiang, Lingxi; Qi, Yao; Yang, Lei; Miao, Yangbao; Ren, Weiming; Liu, Hongmei; Huang, Yi; Huang, Shan; Chen, Shiyin; Shi, Yi; Cai, Lulu

doi:10.1016/j.ajps.2023.100852

Cited by 5 publications

(5 citation statements)

References 216 publications

(229 reference statements)

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“…Therapy-induced modulation of the TME has received considerable attention in recent years. 3,5,6 The inflammatory activation of the TME, which has the potential to recruit endogenous immune cells, has emerged as an efficient approach to support conventional cancer treatment. 43,44 Many works have demonstrated that sono/phototherapy could reshape the TME by inducing immunogenic cell death, 45,46 and the phenotypes of TAMs could be repolarized from M2 TAMs to M1 TAMs in response to the reprogrammed TME.…”

Section: Resultsmentioning

confidence: 99%

“…Therapy-induced modulation of the TME has received considerable attention in recent years. ,, The inflammatory activation of the TME, which has the potential to recruit endogenous immune cells, has emerged as an efficient approach to support conventional cancer treatment. , Many works have demonstrated that sono/phototherapy could reshape the TME by inducing immunogenic cell death, , and the phenotypes of TAMs could be repolarized from M2 TAMs to M1 TAMs in response to the reprogrammed TME. , However, the combined effects of sonophototherapy on TME modulation have not been elucidated before. A-BDP has demonstrated the ability to detect the macrophage-targeted immune responses by monitoring the NO released by M1 TAMs .…”

Section: Resultsmentioning

confidence: 99%

“…Recently, therapy-induced modulation of TME to overcome TME-related resistance has been proposed, which could be opportunities for cancer treatment . Many therapeutic modalities have been reported to induce antitumor immune responses with TME modulation ability. − Sono/phototherapies are noninvasive modalities using exogenous light or low-intensity ultrasound to combat tumors with low incidence of drug resistance, high spatiotemporal selectivity, and good biosafety . TAMs are one of the largest communities in immune cells, which can exist in two different subtypes, M1-polarized phenotype (pro-inflammatory and tumor-suppressing) and M2-polarized phenotype (immunosuppressive and tumor-promoting) .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming

Li,

Sun,

Chen

et al. 2024

ACS Nano

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Therapy-induced modulation of the tumor microenvironment (TME) to overcome the immunosuppressive TME is considered to be an opportunity for cancer treatment. However, monitoring of TME modulation during the therapeutic process to accurately determine immune responses and adjust treatment plans in a timely manner remains to be challenging. Herein, we report a carrier-free nanotheranostic system (CANPs) assembled by two boron dipyrromethene (BODIPY) dyes, a sonophotosensitizer C-BDP, and a nitric oxide (NO) probe amino-BODIPY (A-BDP). CANPs can exert combined sonophototherapeutic effects of C-BDP under ultrasound and light irradiation and simultaneously induce inflammatory TME, as well as emit bright fluorescence via A-BDP by monitoring tumor-associated macrophages (TAMs) repolarization through the released NO in vitro and in vivo. Of note, transforming growth factor-β (TGF-β) could be the key cytokine involved in the sonophototherapy-induced TME reprogramming. By virtue of high physiological stability, good biocompatibility, and effective tumor targetability, CANPs could be a potential nanotheranostic system for the simultaneous induction and detection of TME reprogramming triggered by sonophototherapy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming

Li,

Sun,

Chen

et al. 2024

ACS Nano

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“…Numerous studies have established that the immune microenvironment of tumors significantly impacts tumor progression, recurrence, and patient prognosis 55 , 56 . In cancer patients, the frequency of lymphocyte infiltration in tumors independently predicts survival and lymph node metastasis 22 – 24 Our findings suggest that KDELR overexpression in LUAD may negatively regulate CD8 + T cell infiltration.…”

Section: Discussionmentioning

confidence: 99%

Exploring the potential biological significance of KDELR family genes in lung adenocarcinoma

Li,

Cui,

Yue

et al. 2024

Sci Rep

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The Lys-Asp-Glu-Leu receptor (KDELR) family genes play critical roles in a variety of biological processes in different tumors. Our study aimed to provide a comprehensive analysis of the potential roles of KDELRs in lung adenocarcinoma (LUAD). Utilizing data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database, as well as clinical samples, we conducted a series of analyses and validations using R software tools and various online resources. The results showed that KDELR family genes and proteins were highly expressed and associated with a poor prognosis of LUAD. Promoter hypomethylation and the competing endogenous RNA (ceRNA) network of PCAT6/hsa-miR-326/KDELR1 might be potential causes of aberrant KDELR1 overexpression in LUAD. Three key Transcription factors (TFs) (SPI1, EP300, and MAZ) and a TFs-miRNAs-KDELRs network (involving 11 TFs) might be involved in modulating KDELRs expression abnormalities. Gene Set Enrichment Analysis (GSEA) indicated enrichment of genes highly expressing KDELR1, KDELR2, and KDELR3 in MTORC1_SIGNALING, P53_PATHWAY, and ANGIOGENESIS. Negative correlations between KDELRs expression and CD8 + T cell infiltration, as well as CTLA-4 expression. Our multiple analyses suggested that the KDELRs are important signaling molecules in LUAD. These results provided novel insights for developing prognostic markers and novel therapies of LUAD.

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“…RNA interference (RNAi) is an effective method that is widely used in functional genomics [ 9 , 10 , 11 ], including genetic screening, genetic function identification, signal transduction, and gene therapy. RNAi refers to the efficient and specific degradation of mRNA induced by double-stranded RNA (dsRNA) of identical sequences, finally leading to gene silencing.…”

Section: Traditional Screening Techniquesmentioning

confidence: 99%

The Current Situation and Development Prospect of Whole-Genome Screening

Yang,

Lei,

Ren

et al. 2024

IJMS

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High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas9-mediated genome knock-out and dCas9-based gene activation and interference. We compare these methods with previous genetic screening techniques based on RNAi and cDNA library overexpression and propose future prospects and applications for CRISPR screening.

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Remodeling the tumor immune microenvironment via siRNA therapy for precision cancer treatment

Cited by 5 publications

References 216 publications

Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming

Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming

Exploring the potential biological significance of KDELR family genes in lung adenocarcinoma

The Current Situation and Development Prospect of Whole-Genome Screening

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