Remodeling of Lipid Droplets during Lipolysis and Growth in Adipocytes

Paar, Margret; Jungreuthmayer, Christian; Steiner, Noemi A.; Magnes, Christoph; Sinner, Frank; Kolb, Dagmar; Lass, Achim; Zimmermann, R.; Zumbusch, Andreas; Kohlwein, Sepp D.; Wolinski, Heimo

doi:10.1074/jbc.m111.316794

Cited by 154 publications

(158 citation statements)

References 60 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…Time-lapse studies revealed that the formation of large LDs during adipogenesis involves LD fusion by lipid transfer characteristic of CIDE proteins [19,46]. CIDEA and CIDEC appear as the key elements controlling the formation of supersized LDs in adipocytes, as CIDEA/CIDEC double-deficient mice showed LDs of circa 0.6 m, which could represent the diameter achieved by CIDE-independent enlargement mechanisms [47].…”

Section: Ld Enlargement In Brown White and Brite Adipocytesmentioning

confidence: 99%

Lipid droplet growth: regulation of a dynamic organelle

Barneda

Christian

2017

Current Opinion in Cell Biology

View full text Add to dashboard Cite

Intracellular lipid droplets (LDs) are remarkably dynamic and complex organelles that enact regulated storage and release of lipids to fulfil their fundamental roles in energy metabolism, membrane synthesis and provision of lipid-derived signaling molecules.Although small LDs are observed in all types of eukaryotic cells, it is adipocytes that present the widest range of sizes up to the massive unilocular droplet of a white adipocyte. Our knowledge of the proteins and associated processes that control LD dynamics is improving. The dynamic expression of LD-associated proteins is vital for controlling LD biology and is most apparent during adipocyte differentiation. Recent findings on the molecular mechanisms of lipid droplet enlargement reveal the importance of distinct functional groups of proteins and phospholipids.Highlights (max 5 points 85 characters each) Lipid droplet fusion involves major changes in the LD membrane components.Phosphatidic Acid is a key regulator of LD dynamics.Adipocyte differentiation invokes profound regulation of lipid droplet proteins.Transitions between white and BRITE adipocytes require lipid droplet remodelling.

show abstract

Section: Ld Enlargement In Brown White and Brite Adipocytesmentioning

confidence: 99%

Lipid droplet growth: regulation of a dynamic organelle

Barneda

Christian

2017

Current Opinion in Cell Biology

View full text Add to dashboard Cite

show abstract

“…1 Hypertrophy of mature adipose cells and recruitment of new pre-adipose cells is crucial for lipid storage. 2 If adipose tissue cannot store ingested caloric excess, insulin action and normal metabolic flexibility of energy homeostasis is impacted negatively. 3 Thus, understanding the dynamic response of adipose tissue to changes in diet has attracted attention, 4 especially in the context of the ongoing worldwide increase in obesity and resulting public health issues.…”

Section: Introductionmentioning

confidence: 99%

Adipose cell hypertrophy precedes the appearance of small adipocytes by 3 days in C57BL/6 mouse upon changing to a high fat diet

Periwal

Cushman

et al. 2015

Adipocyte

View full text Add to dashboard Cite

(2016) Adipose cell hypertrophy precedes the appearance of small adipocytes by 3 days in C57BL/6 mouse upon changing to a high fat diet, Adipocyte, 5:1, 81-87, DOI: 10.1080/21623945.2015 ABSTRACTAdipose tissue is the energy buffer in mammals. The cellularity of adipose tissue has a major role in determining the response of adipose tissue to insulin action. A reduction in the ability of adipose tissue to store ingested caloric excess can lead to dyslipidemia and lipotoxicity, impacting insulin action systemically. The dynamic response of adipose tissue to changes in diet is therefore a crucial aspect of metabolism, and has attracted attention in the context of the ongoing worldwide increase in overweight and obesity and resulting metabolic syndrome dysfunctions. We investigated in a mouse model if there is a specific delay between an increase in caloric intake and the recruitment of new adipocytes, and if there are other changes in adipose tissue dynamics concomitant with such a diet change. By developing a dynamic mathematical model, we found that there is a delay of 3 days between the start of a high fat diet and the recruitment of new adipocytes, and that the rate of fat mass increase modulates lipid turnover and adipose cell hypertrophy.

show abstract

“…To observe lipid accumulation at the subcellular level, well-known nondestructive techniques for lipid visualization have been adapted to seeds. Third harmonic generation microscopy (Débarre et al, 2006) and label-free coherent anti-Stokes Raman scattering microscopy (Paar et al, 2012) allow dyeless observation of LDs but require very specific equipment. Magnetic resonance imaging enables topographic analysis of lipid distribution in cereal grains (Neuberger et al, 2008) and in submillimeter-sized seeds like those of tobacco (Nicotiana tabacum; Fuchs et al, 2013).…”

mentioning

confidence: 99%

Specialization of Oleosins in Oil Body Dynamics during Seed Development in Arabidopsis Seeds

et al. 2014

View full text Add to dashboard Cite

Oil bodies (OBs) are seed-specific lipid storage organelles that allow the accumulation of neutral lipids that sustain plantlet development after the onset of germination. OBs are covered with specific proteins embedded in a single layer of phospholipids. Using fluorescent dyes and confocal microscopy, we monitored the dynamics of OBs in living Arabidopsis (Arabidopsis thaliana) embryos at different stages of development. Analyses were carried out with different genotypes: the wild type and three mutants affected in the accumulation of various oleosins (OLE1, OLE2, and OLE4), three major OB proteins. Image acquisition was followed by a detailed statistical analysis of OB size and distribution during seed development in the four dimensions (x, y, z, and t). Our results indicate that OB size increases sharply during seed maturation, in part by OB fusion, and then decreases until the end of the maturation process. In single, double, and triple mutant backgrounds, the size and spatial distribution of OBs are modified, affecting in turn the total lipid content, which suggests that the oleosins studied have specific functions in the dynamics of lipid accumulation.The seed is a complex, specific structure that allows a quiescent plant embryo to cope with unfavorable germinating conditions and also permits dissemination of the species. To achieve these functions, seeds accumulate reserve compounds that will ensure the survival of the embryo and fuel the growth of the plantlet upon germination. Accumulation of lipids occurs in many eukaryotic cells and is a rather common means of storing carbon and energy. Lipid droplets (LDs) can be found in all eukaryotes, such as yeast (Saccharomyces cerevisiae;

show abstract

Remodeling of Lipid Droplets during Lipolysis and Growth in Adipocytes

Cited by 154 publications

References 60 publications

Lipid droplet growth: regulation of a dynamic organelle

Lipid droplet growth: regulation of a dynamic organelle

Adipose cell hypertrophy precedes the appearance of small adipocytes by 3 days in C57BL/6 mouse upon changing to a high fat diet

Specialization of Oleosins in Oil Body Dynamics during Seed Development in Arabidopsis Seeds

Contact Info

Product

Resources

About