Remineralization of Bovine Dentine in vitro

Abstract

In this paper remineralization of bovine dentine is reported. After demineralization of the dentine in an acidic gel system creating lesions of about 180 μm depth, the tissue was remineralized in a 1.5 mMCa and 0.9 mM phosphate containing solution at pH 7 and 37 °C for 8 or 21 days. The F content in the remineralization solution was 0.02, 2, or 10 ppm as NaF. Samples were analyzed by means of microradiography and scanning electron microscopy. The results show that remineralization (without F added in solution) causes a decrease in mineral loss and in lesion depth. With 2 or 10 ppm in solution, however, a substantial mineral accumulation in the lesion, but particularly on the original dentine surface, was observed. During 3 weeks of remineralization, mineral accumulations of 67 and 70 vol% of mineral for 2 and 10 ppm F in solution, respectively, were found near the original outer surface, with the sound dentine mineral value being 48 vol%. The combined microradiography and scanning electron microscopy data show that the mineral accumulated is for a major part deposited on the dentine tissue and partly inside. The latter deposition occurred both inside the tubules as well as in the intertubular areas. Inside the tubules dense precipitates were observed. Because several differences exist between bovine and human dentine, an extrapolation to the human in vivo situation is speculative. The results indicate that the presence of fluoride in the parts per million range is important for dentine remineralization efficacy and that the outer surface area in dentine can be ‘overremineralized’.

In vitro Study of Remineralization of Dentin: Effects of Ions on Mineral Induction by Decalcified Dentin Matrix

“…Fluoride ions are generally known to promote mineralization [Eanes and Meyer, 1978;Arends et al, 1989Arends et al, , 1990 when fluoride is taken up into hydroxyapatite crystals and converted to fluoroapatite. However, several laboratory studies concerning the crystal growth of calcium phosphates in the presence of fluoride show conflicting results.…”

Evaluation of etched enamel using quantitative light-induced fluorescence

“…Therefore, we standardized the measurement conditions when conducting QLF measurements by fixing camera geometry, focal distance, and environmental conditions. Furthermore, bovine enamel was used as a substitute for human enamel, which exhibits some structural differences from its human counterpart [22]. For example, previous studies have reported that bovine enamel is more porous than human enamel and thus less resistant to acid diffusion.…”

O Uso de Dentes Bovinos como Substitutos de Dentes Humanos em Pesquisas Odontológicas: Uma Revisão da Literatura

“…Imfeld 4 estudou os túbulos dentinários, composição química, propriedades mecânicas da dentina, além das características físicas como densidade, dureza e capacidade térmica da dentina e esmalte bovinos concluindo que até as raízes bovinas podem ser utilizadas como substitutas para os dentes humanos. Embora Schilke et al 12 tenham demonstrado não haver diferença no número e diâmetro dos túbulos dentinários entre os dois tipos de dentes, Arends et al 14 comprovaram que a dentina bovina é mais porosa que a dentina humana. A permeabilidade dentinária está presente tanto na dentina humana quanto na bovina, sendo que esta última apresenta ainda túbulos dentinários mais largos 15 .…”

“…Arends et al 14 utilizaram 48 incisivos bovinos extraídos recentemente para testes de desmineralização dentinária. Os dentes foram cortados até a dentina ficar exposta e a desmineralização foi feita com gel de carboximetilcelulose.…”