Remineralization and Antibacterial Efficacy of Different Concentrations of Aqueous Stevia extract and green tea Solutions in Comparison with Fluoride-based Mouthwash on Initial Enamel Carious Lesion- An Invitro Study

Abstract

Aim: This in vitro study evaluated and compared the remineralizing potential of different herbal extracts aqueous solutions versus fluoride mouthwash by assessing the enamel surface microhardness and antimicrobial susceptibility. Materials and methods:Sixty extracted premolars were used in this study and equally distributed into six groups of 10 teeth each. Group A: teeth treated with 0.5% stevia aqueous solution; Group B: teeth treated 5% stevia aqueous solution; Group C: teeth treated with 0.5% green tea aqueous solution; Group D: teeth treated with 5% green tea aqueous solution; Group E: teeth treated with Fluoride mouthwash as a positive control group; negative control group: teeth not subjected to any treatment and stored in artificial saliva. The teeth of each group were subjected to microhardness assessment at baseline, after 48 hours demineralization, and after 7 days remineralization phase. The antibacterial activities of herbal extracts and fluoride against S. mutants and Lactobacillus were quantitatively measured by an antimicrobial susceptibility test.Results: After 7 days of treatment, the highest mean value was recorded in group D (282.69 Kgf/mm 2 ) with the least mean value recorded in the control group (168.66 Kgf/mm 2 ). The difference between groups was statistically significant (p= 0.001). The 5% green tea extract showed the highest mean value of inhibition zone (10.6 mm) against S. mutants' while fluoride showed the highest mean value of inhibition zone (14.6 mm) against the Lactobacillus. Conclusion:An aqueous solution of 5 % green tea is an effective remineralizing agent with antimicrobial activity against S. mutans.

Remineralization and Antibacterial efficacy of Ashwagandha, Maca and Ginger-based mouthwashes versus Fluoride-based mouthwash on initial enamel caries: An in-vitro study

“…A permanent marker was used to numerically code each tooth at the base for sample identification. Ten milliliters of fake saliva were placed in individual glass containers and kept at 37°C for each set of samples (11) .…”

“…For 14 days, the teeth belonging to Group C were kept in artificial saliva without exposure to any experimental remineralizing substance. It was replaced once a day to reduce the possibility that the artificial saliva might become saturated and impede the course of treatment (11,14) .…”