Remifentanil Preconditioning Reduces Hepatic Ischemia–Reperfusion Injury in Rats via  Inducible Nitric Oxide Synthase Expression

Yang, Li‐Qun; Tao, Kun‐Ming; Liu, Yantao; Cheung, Chun; Irwin, Michael G.; Wong, Gordon Tin Chun; Lv, Hao; Song, Jiangang; Wu, Feixiang; Yu, Weifeng

doi:10.1097/aln.0b013e3182104956

Cited by 61 publications

(40 citation statements)

References 43 publications

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“…Apoptosis plays a critical role in various brain diseases [38,39]. Recent studies showed that remifentanil protected cell apoptosis from ischemia in various organs, such as the kidneys, liver, heart and brain [40][41][42][43] have shown that remifentanil may exert a neuroprotective effect via suppression of ischemia-induced apoptosis in the dentate gyrus, thereby alleviating memory impairment [41]. In this study, we found that remifentanil induced C6 cell apoptosis.…”

Section: Discussionmentioning

confidence: 49%

The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil

et al. 2013

Cellular and Molecular Biology Letters

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Abstract:The aim of malignant glioma treatment is to inhibit tumor cell proliferation and induce tumor cell apoptosis. Remifentanil is a clinical anesthetic drug that can activate the N-methyl-D-aspartate (NMDA) receptor. NMDA receptor signaling activates glycogen synthase kinase-3β (GSK-3β). Discovered some 32 years ago, GSK-3β was only recently considered as a therapeutic target in cancer treatment. The purpose of this study was to assess whether remifentanil can induce the apoptosis of C6 cells through GSK-3β activation. 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was used to detect cell viability. Hoechst 33342 staining and flow cytometry were used to detect cell apoptosis. The effect of GSK-3β activation was detected using a GSK-3β activation assay kit and 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD-8), a potent and selective small molecule inhibitor of GSK-3β. The MTT assay indicated that remifentanil induced C6 cell death in a concentration-and time-dependent manner. Hoechst 33342 staining and flow cytometry showed that remifentanil significantly induced C6 cell apoptosis. The measurement of GSK-3β activation showed that remifentanil increased the cellular level of GSK-3β. All of these toxic effects can be attenuated by treatment with TDZD-8. These results suggest that remifentanil is able to induce C6 cell apoptosis through GSK-3β activation, which provides a basis for its potential use in the treatment of malignant gliomas.

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Section: Discussionmentioning

confidence: 49%

The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil

et al. 2013

Cellular and Molecular Biology Letters

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“…Studies have shown remifentanil has a protective effect against ischemic injury 9,19 . In a study of remifentanil preconditioning in liver I/R, the authors reported a protective effect as suggested by decreased apoptosis and less tissue damage, which is in agreement with our findings…”

Section: Resultsmentioning

confidence: 99%

“…Remifentanil can suppress the inflammatory response in a septic mouse model, reduce hepatic I/R injury, and can be a preferable agent in liver transplantation 9 . Thus, this analgesic can also provide protection in the uterus.…”

Section: Introductionmentioning

confidence: 99%

Remifentanil protects uterus against ischemia-reperfusion injury in rats

et al. 2015

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PURPOSE:To investigate the effects of remifentanil as an antioxidant and analyze the histopathologic, biochemical changes in experimental ischemia-reperfusion (I/R) exposed rat uteri. METHODS:Wistar albino rats were assigned to three groups (n = 7). 2h period of ischemia was followed by 1h of reperfusion in the I/R and the I/R-remifentanil groups. After ischemia, no drug was administered in the sham and I/R groups. In the I/R-remifentanil group, remifentanil infusion (2 μg/kg/min) was started in the ischemia period, and continued until the end of reperfusion. After the ischemic and reperfusion period, the ischemic uterine horns were removed surgically for biochemical and histopathologic examination.Tissue damage scores (endometrial epithelial glandular leukocytosis, degeneration, and endometrial stromal changes) were examined.Malondialdehyde levels and catalase, superoxide dismutase enzyme activities in tissue were measured. RESULTS:We found significantly lower epithelial leukocytosis and cell degeneration in the I/R-remifentanil group (p<0.05).Remifentanil administration significantly decreased concentrations of malondialdehyde, and increased catalase and superoxide dismutase enzyme activities (p<0.05).CONCLUSION: Remifentanil appears to protect the uterine tissue against ischemia-reperfusion and can be used safely in uterus transplantation.

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“…Because nitric oxide is essential for cellular signalling, it is involved in many physiological and pathological processes including inflammation (Leiper & Nandi, 2011), protection of liver ischemic damage (Yang et al, 2011), carcinogenesis and rheumatoid arthritis (Melchers et al, 2006). Therefore, inhibition of nitric oxide may suggest the mechanism of saponin against inflammation.…”

Section: Discussionmentioning

confidence: 99%

In vitro anti-inflammatory and free radical scavenging activities of crude saponins extracted from Albuca bracteataJacq. Bulb.

Odeyemi¹,

Afolayan²,

Bradley³

2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Albuca bracteata is a medicinal plant traditionally used in the management of diabetes mellitus in the Eastern Cape of South Africa. The purpose of this study was to evaluate the antioxidant and anti-inflammatory activities of saponins extracted from the bulb of Albuca bracteata and compared with the crude methanolic extract. Methods: In vitro antioxidant activity was determined using free radical scavenging assays such as DPPH, ABTS and NO 2 . The antiinflammatory potential was carried out using inhibition of protein denaturation of egg albumin as a model of anti-inflammatory capacity. Results: Both the crude methanolic extract and saponins showed inhibition of DPPH, ABTS and NO 2 scavenging activity. However, the free radical scavenging activity of isolated saponin compared favourably with Rutin and BHT. The crude methanolic extract showed higher inhibition percentage of protein denaturation compared with the saponins at the concentration investigated. Conclusion: This study indicates that saponin from Albuca bracteata bulb possess potent anti-inflammatory activity and is also a good source of natural antioxidant.

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Remifentanil Preconditioning Reduces Hepatic Ischemia–Reperfusion Injury in Rats via Inducible Nitric Oxide Synthase Expression

Abstract: Pretreatment with remifentanil can attenuate liver injury both in vivo and in vitro. Inducible NOS but not opioid receptors partly mediate this effect by exhausting reactive oxygen species and attenuating the inflammatory response.

Cited by 61 publications

References 43 publications

The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil

The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil

Remifentanil protects uterus against ischemia-reperfusion injury in rats

<i>In vitro</i> anti-inflammatory and free radical scavenging activities of crude saponins extracted from <i>Albuca bracteata</i>Jacq. Bulb.

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