Remating inhibition in female Queensland fruit flies: Effects and correlates of sperm storage

Harmer, Aaron M. T.; Radhakrishnan, Preethi; Taylor, Phillip W.

doi:10.1016/j.jinsphys.2005.10.003

Cited by 71 publications

(93 citation statements)

References 26 publications

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“…The same was found in A. ludens and A. obliqua when the sugar/yeast ratio was 9:1 or 24:1, but not with the 3:1 ratio ) Inhibition of remating Feeding on a nitrogen-rich food significantly improved the ability of sterile males to inhibit female remating in A. fraterculus, B. tryoni and C. capitata (Harmer et al 2006;Radhakrishnan and Taylor 2007;Gavriel et al 2009;Segura et al 2013 The contribution of microorganisms to fitness of non-sterile Bactrocera oleae and C. capitata was established Ben-Yosef et al 2008aYuval et al 2013) Manipulating the microflora of sterile males suggests that probiotic supplements could improve male sexual performance in C. capitata (Niyazi et al 2004;Behar et al 2005Behar et al , 2008aBen-Ami et al 2010) these phenomena, to extend them to other species, and to transfer and validate them under large-scale conditions of action SIT programmes. Results obtained during the CRP have confirmed the compounds that have the potential to improve sterile male performance in the field and have identified some additional ones.…”

Section: Semiochemical Supplementsmentioning

confidence: 58%

Improving sterile male performance in support of programmes integrating the sterile insect technique against fruit flies

Pereira

Yuval

Liedo

et al. 2011

J Applied Entomology

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The sterile insect technique (SIT) is being applied against fruit fly pests in many areas of the world. Currently, factories have a capacity to produce several billion sterile insects per week, to irradiate them and to make them available for shipment to their destinations. There the emerging flies are fed and maintained for various periods of time in fly emergence and release facilities, and then collected for release. While much research effort has been invested in improving mass rearing and quality control procedures at the fly factory level, the post‐factory handling of sterile flies has received much less attention. A 6‐year FAO/IAEA Coordinated Research Project (CRP) was initiated with the participation of 31 research institutes from 17 countries to assess and validate ways to improve sterile male performance through better management during the critical period that starts with the arrival of pupae at the fly emergence and release facility and ends with the release of the sterile flies in the field. This review summarizes the research that was conducted under the CRP focusing on fruit fly species from the genera Anastrepha, Bactrocera and Ceratitis against which the SIT is being applied. To increase the effectiveness of SIT programmes, exposure of sterile males to nutritional, hormonal and semiochemical supplements was assessed and improved handling and release methods tested. Incorporation of protein and juvenile hormone into pre‐release diets significantly accelerated sterile male maturation and improved sexual performance among several species, while semiochemical treatments using ginger root oil or citrus oils in Ceratitis capitata and methyl eugenol in Bactrocera dorsalis complex species significantly increased sterile male mating competitiveness. Improved fly emergence, holding and release procedures were also assessed, together with the compilation of all this knowledge into a manual. Several of these findings have been transferred to and are being applied in operational programmes.

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Section: Semiochemical Supplementsmentioning

confidence: 58%

Improving sterile male performance in support of programmes integrating the sterile insect technique against fruit flies

Pereira

Yuval

Liedo

et al. 2011

J Applied Entomology

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“…If females mated by released sterile males have a high tendency to remate then they are available to be mated by fertile wild males and might then produce viable offspring. The importance of remating inhibition is exacerbated by the tendency of female Q-flies to store far fewer sperm when they mate with sterile rather than fertile males ( Harmer et al , 2006 ). If females do mate twice (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…The oncemated females and their second prospective mate were observed for mating from 15 min before the onset of simulated dusk. As soon as intromission was observed, pairs were rapidly chilled with aerosol freezing spray (containing 1,1 difluoroethane; Dick Smith Electronics/Electrolube, Australia) and the male's aedeagus was severed with a pair of fine scissors to prevent further sexual activity and to ensure that no sperm were transferred ( Harmer et al , 2006 ). The males used in the remating trials were then frozen and the females were dissected to determine the presence and number of sperm from their first mating.…”

Section: Mating Experimentsmentioning

confidence: 99%

“…The corners of the cover slip were secured with a small drop of clear nail polish and the slide was set aside to dry. Rather than counting all of the sperm in each sample ( Harmer et al , 2006;Perez-Staples et al , 2007a ), we adopted the more timeefficient approach of subsampling the slides ( Taylor et al , , 2001. Sperm was sub-sampled by counting 16 fields (as four rows of four fields) across each cover slip under a phase contrast microscope (Leica DME, Germany) at × 100 magnification.…”

Section: Mating Experimentsmentioning

confidence: 99%

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Potential for pre‐release diet supplements to increase the sexual performance and longevity of male Queensland fruit flies

Pérez‐Staples

Harmer

Collins

et al. 2008

Agri and Forest Entomology

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1 Recent studies have shown that continuous access to a protein source (yeast hydrolysate) can greatly enhance the sexual performance of male Queensland fruit flies ( Bactrocera tryoni ; 'Q-flies'). However, in Sterile Insect Technique programmes used to eradicate or suppress wild populations, mass-reared Q-flies are typically fed only sucrose and water for up to 2 days before release. 2 We investigated whether adding a protein source to the diet of male Q-flies for a 24-or 48-h window after emergence and then removing it is sufficient to enhance mating probability, latency to mate, copula duration, probability of sperm storage, number of sperm stored, female remating tendency and longevity of male Q-flies. 3 Protein-fed males were more likely to mate than males fed only sucrose, especially when young. Protein-fed males also had shorter mating latencies and longer copulations than protein-deprived males. 4 Females mated by protein-fed males were more likely to store sperm, stored more sperm and were less likely to remate than were females mated by proteindeprived males. Females were also less likely to remate if their first mate had been large. 5 Overall, providing male Q-flies access to a protein source for a 24-or 48-h window early on in their adult life was sufficient to greatly enhance all assessed measures of performance. Although 24-h access was sufficient for a notable enhancement, further benefits were evident in males provided 48-h access. 6 The results are discussed in terms of the practical implications for Sterile Insect Technique programs used to eradicate or suppress wild Q-fly populations.

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“…Nevertheless, gamma irradiation often does decrease sperm numbers (e.g., Harmer et al 2006;Radhakrishnan et al 2009), and this decrease may be particularly drastic if males mate repeatedly. Male multiple matings could result in aspermia (Hooper 1989) and possible ejaculate depletion.…”

mentioning

confidence: 99%

Tapachula-7, a New Genetic Sexing Strain of the Mexican Fruit Fly (Diptera: Tephritidae): Sexual Compatibility and Competitiveness

Orozco¹,

Meza²,

Zepeda³

et al. 2013

jnl. econ. entom.

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Remating inhibition in female Queensland fruit flies: Effects and correlates of sperm storage

Cited by 71 publications

References 26 publications

Improving sterile male performance in support of programmes integrating the sterile insect technique against fruit flies

Improving sterile male performance in support of programmes integrating the sterile insect technique against fruit flies

Potential for pre‐release diet supplements to increase the sexual performance and longevity of male Queensland fruit flies

Tapachula-7, a New Genetic Sexing Strain of the Mexican Fruit Fly (Diptera: Tephritidae): Sexual Compatibility and Competitiveness

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