Remarks by: Henry J. Richardson, III

Richardson, Henry J.

doi:10.1017/s0272503700085323

Cited by 2 publications

(2 citation statements)

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“…Alkenes, phenolic compounds, and organic acids have the strongest affinity for carbon deposition on catalyst surfaces due to their propensity to participate in polymerization and polycondensation chemistry at hydrotreatment reaction conditions. Coking can be partially ameliorated by proper choice of catalyst, reaction pressure and temperature (Fonseca et al 1996 ; Richardson et al 1995 ).…”

Section: Potential Conversion Technologies For Coffee and Other Agricmentioning

confidence: 99%

Sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept

Hughes

López-Núñez²,

Jones

et al. 2014

Appl Microbiol Biotechnol

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The environmental impact of agricultural waste from the processing of food and feed crops is an increasing concern worldwide. Concerted efforts are underway to develop sustainable practices for the disposal of residues from the processing of such crops as coffee, sugarcane, or corn. Coffee is crucial to the economies of many countries because its cultivation, processing, trading, and marketing provide employment for millions of people. In coffee-producing countries, improved technology for treatment of the significant amounts of coffee waste is critical to prevent ecological damage. This mini-review discusses a multi-stage biorefinery concept with the potential to convert waste produced at crop processing operations, such as coffee pulping stations, to valuable biofuels and bioproducts using biochemical and thermochemical conversion technologies. The initial bioconversion stage uses a mutant Kluyveromyces marxianus yeast strain to produce bioethanol from sugars. The resulting sugar-depleted solids (mostly protein) can be used in a second stage by the oleaginous yeast Yarrowia lipolytica to produce bio-based ammonia for fertilizer and are further degraded by Y. lipolytica proteases to peptides and free amino acids for animal feed. The lignocellulosic fraction can be ground and treated to release sugars for fermentation in a third stage by a recombinant cellulosic Saccharomyces cerevisiae, which can also be engineered to express valuable peptide products. The residual protein and lignin solids can be jet cooked and passed to a fourth-stage fermenter where Rhodotorula glutinis converts methane into isoprenoid intermediates. The residues can be combined and transferred into pyrocracking and hydroformylation reactions to convert ammonia, protein, isoprenes, lignins, and oils into renewable gas. Any remaining waste can be thermoconverted to biochar as a humus soil enhancer. The integration of multiple technologies for treatment of coffee waste has the potential to contribute to economic and environmental sustainability.

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Section: Potential Conversion Technologies For Coffee and Other Agricmentioning

confidence: 99%

Sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept

Hughes

López-Núñez²,

Jones

et al. 2014

Appl Microbiol Biotechnol

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“…Microorganisms generate energy through substrate‐level phosphorylation in anaerobic conditions, which is not as efficient as oxidative respiration under aerobic conditions. It is well‐known that other inorganic substrates, such as nitrate and sulfate, also act as final electron acceptors (reducing agents) in the absence of oxygen . In this study, a naturally existing anaerobic respiration system was utilized to facilitate semi‐anaerobic production of isobutanol.…”

Section: Discussionmentioning

confidence: 99%

Formate and Nitrate Utilization in Enterobacter aerogenes for Semi‐Anaerobic Production of Isobutanol

Jung

Kim

2017

Biotechnology Journal

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Anaerobic bioprocessing is preferred because of its economic advantages. However, low productivity and decreased growth of the host strain have limited the use of the anaerobic process. Anaerobic respiration can be applied to anoxic processing using formate and nitrate metabolism to improve the productivity of value-added metabolites. A isobutanol-producing strains is constructed using Enterobacter aerogenes as a host strain by metabolic engineering approaches. The byproduct pathway (ldhA, budA, and pflB) is knocked out, and heterologous keto-acid decarboxylase (kivD) and alcohol dehydrogenase (adhA) are expressed along with the L-valine synthesis pathway (ilvCD and budB). The pyruvate formate-lyase mutant shows decreased growth rates when cultivated in semi-anaerobic conditions, which results in a decline in productivity. When formate and nitrate are supplied in the culture medium, the growth rates and amount of isobutanol production is restored (4.4 g L , 0.23 g g glucose, 0.18 g L h ). To determine the function of the formate and nitrate coupling reaction system, the mutant strains that could not utilize formate or nitrate is contructed. Decreased growth and productivity are observed in the nitrate reductase (narG) mutant strain. This is the first report of engineering isobutanol-producing E. aerogenes to increase strain fitness via augmentation of formate and nitrate metabolism during anaerobic cultivation.

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Remarks by: Henry J. Richardson, III

Cited by 2 publications

References 0 publications

Sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept

Sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept

Formate and Nitrate Utilization in Enterobacter aerogenes for Semi‐Anaerobic Production of Isobutanol

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