Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe³⁺ in human blood samples and cells

Abstract

The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe 3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro.Significantly trace levels of Fe 3+ ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe 3+ . As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe 3+ in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe.

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

“…Dye RG5NC (109) was similarly prepared from rhodamine 6G. 193 It was then employed to monitor labile Fe(III) levels in embryonic mouse fibroblasts (NIH-3T3).…”

“…A number of BODIPY-based turn-off probes, such as 190− 193, have been described, which commonly operate by PET quenching or static quenching. 333−336 Notably, BDPA (193) was reported by He, Guo, and colleagues for Cu(II) imaging in live mice. 336 It emits in the NIR region (700 nm), with high quantum yield and high photostability.…”

“…Confocal microscopy of HBL-100 human breast carcinoma cells confirmed its ability to act as an intracellular imaging agent upon addition of Fe­(III). Dye RG5NC ( 109 ) was similarly prepared from rhodamine 6G . It was then employed to monitor labile Fe­(III) levels in embryonic mouse fibroblasts (NIH-3T3).…”

Selective Identification of Ferric Ions in Ecological and Biological Samples Using Rhodamine Chemosensors

“…Geetha Balakrishna R. and group demonstrated a robust reversible biocompatible Rhodamine 6G based probe 19 for the recognition of Fe 3+ ions [44].…”

Analytical and Biological Evaluation of Chromium Complex with Organic Detector NTADBrP: Stability, Calibration, and Inhibition Studies

“…In this context, the present research investigates a complex consisting of chromium (iii) and the thiazole derivative 2-2-(5-nitro thiazolyl) azo-4,6-dibromo phenol (NTADBrP). The study centers on the ability of this complex to inhibit Escherichia coli and Staphylococcus bacteria [18]. The objective is to uncover the underlying properties and mechanisms that enable the chromium-thiazole complex to suppress specific bacterial activity, thus contributing to the potential development of novel antibacterial agents.…”