Remarkable Anticancer Activity of <i>Teucrium polium</i> on Hepatocellular Carcinogenic Rats

Movahedi, Ariyo; Basir, Rusliza; Rahmat, Asmah; Charaffedine, Mohammad; Othman, Fauziah

doi:10.1155/2014/726724

Cited by 22 publications

(20 citation statements)

References 60 publications

(56 reference statements)

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“…These biochemical findings were in agreement with our histological data demonstrating a reduction ( Gq or NAC) or a full abrogation ( Tp ) of structural liver abnormalities. Similarly, a beneficial effect of Teucruim spiece on AST and ALT release and liver histology, has been reported in rat liver cancer and APAP toxicity in mice [ 33 , 34 ]. In the same line, Yousef et al [ 14 ] have reported that NAC, curcumin or quercetin normalizes transaminases levels and restores liver histology in APAP-intoxicated rats, thus suggesting that antioxidant compounds occurring in Gq or Tp extract are involved in the beneficial influence of the extracts observed in our study.…”

Section: Discussionmentioning

confidence: 87%

“…In addition, it is traditionally considered as a nutriment for gastrointestinal function improvement in agreement with in vitro and in vivo demonstrated properties such as antispasmodic, antidiarrheal or ulcer protective activities [ 26 , 29 , 30 , 31 ]. Furthermore, numerous studies have proved antioxidant [ 32 ], anticancer [ 33 ] and hepatoprotective [ 34 ] activities of Teucrium polium . The benefit of Teucrium species is considered to be linked to the presence active compounds such as essential oils [ 35 ] and flavonoids [ 36 ] with biological and pharmacological activities.…”

Section: Introductionmentioning

confidence: 99%

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Protective Activity of Total Polyphenols from Genista quadriflora Munby and Teucrium polium geyrii Maire in Acetaminophen-Induced Hepatotoxicity in Rats

et al. 2016

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Oxidative stress is a major cause of drug-induced hepatic diseases and several studies have demonstrated that diet supplementation with plants rich in antioxidant compounds provides a variety of health benefits in these circumstances. Genista quadriflora Munby (Gq) and Teucrium polium geyrii Maire (Tp) are known to possess antioxidant and numerous biological properties and these endemic plants are often used for dietary or medicinal applications. Herein, we evaluated the beneficial effect of rich-polyphenol fractions of Gq and Tp to prevent Acetaminophen-induced liver injury and investigated the mechanisms involved in this protective action. Rats were orally administered polyphenolic extracts from Gq or Tp (300 mg/kg) or N-acetylcysteine (NAC: 200 mg/kg) once daily for ten days prior to the single oral administration of Acetaminophen (APAP: 1 g/kg). The results show that preventive administration of polyphenolic extracts from Gq or Tp exerts a hepatoprotective influence during APAP treatment by improving transaminases leakage and liver histology and stimulating antioxidant defenses. Besides, suppression of liver CYP2E1, GSTpi and TNF-α mRNA levels, with enhancement of mitochondrial bioenergetics may contribute to the observed hepatoprotection induced by Gq and Tp extracts. The effect of Tp extract is significantly higher (1.5–2 fold) than that of Gq extract and NAC regarding the enhancement of mitochondrial functionality. Overall, this study brings the first evidence that pretreatment with these natural extracts display in vivo protective activity against APAP hepatotoxicity through improving mitochondrial bioenergetics, oxidant status, phase I and II enzymes expression and inflammatory processes probably by virtue of their high total polyphenols content.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Protective Activity of Total Polyphenols from Genista quadriflora Munby and Teucrium polium geyrii Maire in Acetaminophen-Induced Hepatotoxicity in Rats

et al. 2016

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“…The activation of apoptosis is one of the main molecular mechanisms responsible for the anti-cancer activities of most of the currently studied potential anti-cancer agents, including natural compounds [3,4]. Several, novel drugs designed to interfere with the proteins regulating the cell cycle, the apoptotic machinery or leukemic microenvironment, are currently being tested in clinical, in vitro or in vivo trials [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Teucrium polium induces apoptosis in peripheral blood lymphocytes isolated from human chronic lymphocytic leukemia

Zarić

Mitrović

et al. 2020

VOJNOSANIT PREGL

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Background/Aim. Chronic lymhocytic leukemia (CLL) is considered more as a disease of cells accumulation due to the defect in apoptosis rather than deregulated cell's proliferation. The activation of apoptosis is one of the main molecular mechanisms responsible for the anti-cancer activities of most of the currently studied potential anti-cancer agents, including natural compounds. Teucrium polium extracts exhibited strong cytotoxic effects in murine leukemia cell line, RAW 264.7 and human melanoma cell line, C32, but its cytotoxic effects against human leukemia cells were unknown. Methods. The viability of human leukemia cell lines (MOLT-4 and JVM-13), CLL lymphocytes isolated from 28 patients (CLL cells), and peripheral blood mononuclear cells (PBMCs) isolated from 16 healthy subjects treated with Teucrium polium extracts, was determined by MTT assay. Apoptosis of Teucrium polium treated CLL cells was measured by flow cytometry applying Annexin V/7AAD staining. The expressions of active proapoptotic protein Bax, antiapoptotic protein Bcl-2, cytochrome c and the percentage of cells containing cleaved caspase-3 in treated CLL cells was determined by flow cytometry and immunocytochemistry. Results. Teucrium polium methanol extract (TP) decreased the viability of all tested human leukemia cells but it had no effect on the viability of healthy PBMCs. The citotoxic effect of TP was caused by its induction of CLL cells' apoptosis. TP disarranged the ratio of the expressions of proapoptotic Bax and antiapoptotic Bcl-2 protein in favor of Bax, consequently inducing apoptosis by cytochrome c mitochondrial release and activation of caspase-3 in treated CLL cells. Conclusion. Teucrium polium induced selective apoptosis in CLL cells and it affected the expressions of key proteins involved in the regulation of programmed cell death. Key words: teucrium polium, in vitro, citotoxicity, cancer, leukemia. ApstraktUvod/Cilj. Hronična limfocitna leukemija (HLL) se pre smatra bolešću akumulacije ćelija usled defekta u njihovoj apoptozi, nego bolešću ćelijske proliferacije. Aktivacija apoptoze je jedan od glavnih molekulskih mehanizama odgovornih za antitumorsku aktivnost većine agenasa koji se sada ispituju, uključujući i agense prirodnog porekla. Ekstrakt Teucrium polium-a je pokazao snaţne citotoksične efekte na ćelije mišje leukemije RAW 264.7 i ćelije humanog melanoma C32, ali su njegovi citotoksični efekti na ćelije humane leukemije nepoznati. Metode. Vijabilnost ćelija humane leukemije (MOLT-4 i JVM-13), HLL limfocita izolovanih iz krvi 28 pacijenata (HLL ćelije) i mononukleara periferne krvi (PBMCs) izolovanih iz krvi 16 zdravih ispitanika je određena MTT esejom nakon tretmana ispitivanih ćelija metanolskim ekstraktom Teucrium polium-a (TP). Apoptoza HLL ćelija tretiranih TP-om merena je protočnom citometrijom korišćenjem Annexin V/7AAD kit-a. Ekspresija aktivnog proapoptotičnog proteina Bax, antiapoptotičnog proteina Bcl-2, citohroma c i procenat ćelija koje sadrţe aktivnu kaspazu-3 u tretiranim HLL ćelijama, merena je proto...

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“…Phenolic and terpenic components extracted from Teucrium species possess the ability to treat obesity, hypercholesterolemia, and diabetes, as well as antiinflammatory, antimicrobial, and anticancer properties [ 25 ]. T. polium protects liver cells against hepatocellular carcinoma in carcinogenesis-induced animal models [ 26 ]. It has been shown to be an effective and safe chemosensitizer agent for cancer therapy [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Terpenes from essential oils and hydrolate ofTeucrium alopecurustriggered apoptotic events dependent on caspases activation and PARP cleavage in human colon cancer cells through decreased protein expressions

et al. 2018

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This study focused on characterizing the Hydrophobic and Hydrophilic fractions of Teucrium alopecurus in the context of cancer prevention and therapy. The goal was also to elucidate the molecular mechanisms involved and to determine its efficacy against cancer by triggering apoptosis and suppressing tumorigenesis in human colon cancer. The data here clearly demonstrated that oily fractions of Teucrium alopecurus act as free radical scavengers, antibacterial agent and inhibited the proliferation of HCT-116, U266, SCC4, Panc28, KBM5, and MCF-7 cells in a time- and concentration-dependent manner. The results of live/dead and colony formation assays further revealed that Teucrium essential oil has the efficacy to suppress the growth of colon carcinoma cells. In addition, essential oil of Teucrium alopecurus induced apoptosis, as indicated by cleavage of caspases-3, -8, and -9 and poly-adenosine diphosphate ribose polymerase. Moreover, Teucrium alopecurus essential oil suppressed gene expression involved in survival, proliferation, invasion, angiogenesis, and metastasis in human colon cancer cells. No sign of toxicity was detected in vivo after treatment with increasing concentrations of essential oil. Oral administration of T.alopecurus inhibited LPS-induced colon inflammation. This anticancer property of this specie Teucrium alopecurus fractions could be due to their phenolic and/or sesquiterpene content (d-limonene, α-Bisabolol, Humulene, Thymol, and (+)-epi-Bicyclosesquiphellandrene). Hence our study reveals the anticancer activity of Teucrium alopecurus oil mediated through the suppression of cell growth, cell proliferation, and the induction of apoptosis of cancer cells. Thus, it has potential to be developed as an anticancer agent; however more in vitro and in vivo studies are warranted.

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Remarkable Anticancer Activity of Teucrium polium on Hepatocellular Carcinogenic Rats

Cited by 22 publications

References 60 publications

Protective Activity of Total Polyphenols from Genista quadriflora Munby and Teucrium polium geyrii Maire in Acetaminophen-Induced Hepatotoxicity in Rats

Protective Activity of Total Polyphenols from Genista quadriflora Munby and Teucrium polium geyrii Maire in Acetaminophen-Induced Hepatotoxicity in Rats

Teucrium polium induces apoptosis in peripheral blood lymphocytes isolated from human chronic lymphocytic leukemia

Terpenes from essential oils and hydrolate ofTeucrium alopecurustriggered apoptotic events dependent on caspases activation and PARP cleavage in human colon cancer cells through decreased protein expressions

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