REM1.3's phospho-status defines its plasma membrane nanodomain organization and activity in restricting PVX cell-to-cell movement

Perraki, Artemis; Gronnier, Julien; Gouguet, Paul; Boudsocq, Marie; Deroubaix, Anne-Flore; Simon, Vincent; German-Retana, Sylvie; Legrand, Anthony; Habenstein, Birgit; Zipfel, Cyril; Bayer, Emmanuelle; Mongrand, Sébastien; Germain, V.

doi:10.1371/journal.ppat.1007378

Cited by 73 publications

(126 citation statements)

References 109 publications

(198 reference statements)

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“…To examine the influence of REM1.2 on the cell‐to‐cell movement of TuMV, the homozygous T‐DNA insertion line rem1.2‐2 was inoculated with TuMV‐GFP. As both REM1.2 and REM1.3 interacted with PCaP1 (Figs , S5) and function similarly (Raffaele et al ., ; Perraki et al ., ), the homozygous T‐DNA insertion line rem1.3‐2 (SALK_117448) was also inoculated with TuMV‐GFP. The 4‐wk‐old Col‐0 and mutants inoculated with TuMV‐GFP were photographed under white light or UV illumination at 15 dpi.…”

Section: Resultsmentioning

confidence: 99%

“…), which might alter the lateral segregation of PCaP1 on NDs, thereby releasing PCaP1 from the restriction of REM1.2 or PtdInsPs to sever the actin filaments (Nagasaki et al ., ; Qin et al ., ). Depolymerisation of the actin filaments disrupts the NDs, thereby redistributing REM1.2 from the detergent‐resistant membranes (DRMs) to the detergent‐soluble membranes (DSMs) (Szymanski et al ., ), a process might be enhanced by the interference of REM1.2’s phosphorylation or S‐acylation upon viral infection (Mehlmer et al ., ; Marin & Ott, ; Hemsley et al ., ; Konrad et al ., ; Perraki et al ., ). The disruption of NDs also redistributes CalS, PDCB or PDBG (Simpson et al ., ; Benitez‐Alfonso et al ., ; Grison et al ., ), whose activities might be modulated in synchronising with Ca 2+ concentration reduction, to degrade PD callose.…”

Section: Discussionmentioning

confidence: 97%

“…The intercellular movement of 6K2‐induced vesicles of TuMV may be dependent on the vesicular transport pathway (Movahed et al ., ) based on the disruption of the actomyosin motility system by PCaP1 in PD. Group 1 REMs interfere with the cell‐to‐cell movement of PVX (Raffaele et al ., ; Perraki et al ., ; Perraki et al ., ), TMV (Perraki et al ., ), or RSV (Fu et al ., ). In this work, we show that REM1.2 or REM1.3 knockouts promote TuMV infection, while overexpression of REM1.2 negatively regulates the cell‐to‐cell movement but not the replication of TuMV (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Intriguingly, although group 1 REMs negatively regulate infection by PVX, TMV, or RSV, which eventually establish systemic infection on their compatible hosts (Raffaele et al, 2009;Perraki et al, 2012;Perraki et al, 2014;Fu et al, 2018;Perraki et al, 2018). Not surprisingly, NbREM1.1 and OsREM1.4 are degraded via an autophagy pathway mediated by the interaction with NSvc4, a MP encoded by RSV (Fu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

et al. 2019

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Group 1 Remorins (REMs) are extensively involved in virus trafficking through plasmodesmata (PD). However, their roles in Potyvirus cell-to-cell movement are not known. The plasma membrane (PM)-associated Ca 2+ binding protein 1 (PCaP1) interacts with the P3N-PIPO of Turnip mosaic virus (TuMV) and is required for TuMV cell-to-cell movement, but the underlying mechanism remains elusive.The mutant plants with overexpression or knockout of REM1.2 were used to investigate its role in TuMV cell-to-cell movement. Arabidopsis thaliana complementary mutants of pcap1 were used to investigate the role of PCaP1 in TuMV cell-to-cell movement. Yeast-two-hybrid, bimolecular fluorescence complementation, co-immunoprecipitation and RT-qPCR assays were employed to investigate the underlying molecular mechanism.The results show that TuMV-P3N-PIPO recruits PCaP1 to PD and the actin filament-severing activity of PCaP1 is required for TuMV intercellular movement. REM1.2 negatively regulates the cell-to-cell movement of TuMV via competition with PCaP1 for binding actin filaments. As a counteractive response, TuMV mediates REM1.2 degradation via both 26S ubiquitin-proteasome and autophagy pathways through the interaction of VPg with REM1.2 to establish systemic infection in Arabidopsis.This work unveils the actin cytoskeleton and PM nanodomain-associated molecular events underlying the cell-to-cell movement of potyviruses.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

et al. 2019

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“…Recently, a role for CPK in virus resistance was reported for the first time. The active form of AtCPK3 could limit cell-to-cell propagation of potato virus X by phosphorylating the remorin StRem1.3 on S74/T86/S91 (Perraki et al, 2018). These phosphosites were involved in remorin dynamic localization in nanodomains of the plasma membrane and were critical for its function in callose deposition at plasmodesmata.…”

Section: Reviewmentioning

confidence: 99%

Properties and functions of calcium‐dependent protein kinases and their relatives inArabidopsis thaliana

2019

Self Cite

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Summary Calcium is a ubiquitous second messenger that mediates plant responses to developmental and environmental cues. Calcium‐dependent protein kinases (CDPKs) are key actors of plant signaling that convey calcium signals into physiological responses by phosphorylating various substrates including ion channels, transcription factors and metabolic enzymes. This large diversity of targets confers pivotal roles of CDPKs in shoot and root development, pollen tube growth, stomatal movements, hormonal signaling, transcriptional reprogramming and stress tolerance. On the one hand, specificity in CDPK signaling is achieved by differential calcium sensitivities, expression patterns, subcellular localizations and substrates. On the other hand, CDPKs also target some common substrates to ensure key cellular processes indispensable for plant growth and survival in adverse environmental conditions. In addition, the CDPK‐related protein kinases (CRKs) might be closer to some CDPKs than previously anticipated and could contribute to calcium signaling despite their inability to bind calcium. This review highlights the regulatory properties of Arabidopsis CDPKs and CRKs that coordinate their multifaceted functions in development, immunity and abiotic stress responses.

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BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

Jiao

Shi

et al. 2021

Pest Management Science

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BACKGROUND Tobacco mosaic virus (TMV) is one of destructive plant viruses, causing serious economic losses in the world. Using antiviral proteins or elicitors to inhibit viral infection or promote plant immunity is one of the efficient strategies against TMV. Our previous study identified that the fermentation broth of Brevibacillus laterosporus strain B8 showed strong antiviral activity against TMV. However, the active antiviral ingredient is still unclear. RESULTS Here, BLB8 (B. laterosporus strain B8 protein, BLB8), an antiviral protein from B. laterosporus strain B8 was isolated and characterized. BLB8 showed protective, inactive and curative effects against TMV, and the inhibition rate reached up to 63%, 83% and 55%, respectively. BLB8 infiltrated around the infection site of the recombinant virus TMV‐GFP inhibited the systemic extend and movement of TMV. Pretreatment of the bottom leaves with BLB8 inhibited the spread and accumulation of TMV in upper systemic leaves. Furthermore, BLB8 caused hypersensitive response (HR) in a dose‐dependent way, promoted H2O2 accumulation, and induced the expression of defense‐relative genes in Nicotiana benthamiana. CONCLUSION The antiviral protein BLB8 from B. laterosporus strain B8 effectively inhibits TMV infection in inactivation, protective and curative effects through triggering plant immunity in tobacco. Therefore, the present study provides a new antiviral agent for prevention and control of viral disease. © 2021 Society of Chemical Industry.

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REM1.3's phospho-status defines its plasma membrane nanodomain organization and activity in restricting PVX cell-to-cell movement

Cited by 73 publications

References 109 publications

Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

Properties and functions of calcium‐dependent protein kinases and their relatives inArabidopsis thaliana

BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

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