Reliable glucose monitoring by ex-vivo blood microdialysis and infrared spectrometry for patients in critical care

Vahlsing, Thorsten; Delbeck, Sven; Budde, Janpeter; Ihrig, Dieter F.; Leonhardt, Steffen; Heise, H. M.

doi:10.1117/12.2253086

Cited by 3 publications

(6 citation statements)

References 22 publications

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“…Advantageously, this biosensor quantified other small molecules including glycerol. Since then, numerous biosensors coupled to microdialysis for the quantification of molecules found in body fluids, tissues, and organs other than the brain have been reported [ 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. An earlier review of biochemical monitoring of various bodily fluids has been presented elsewhere [ 79 ].…”

Section: Review Of Sensor Technologies For Brain Metabolismmentioning

confidence: 99%

“…Various applications of optical sensing have been performed both in a label-free or label-based manner. For label-based detection, the optical signal is acquired by fluorescent or colorimetric techniques [ 95 , 96 , 97 , 98 ], whereas label-free techniques involve light–analyte interactions to facilitate signal detection exemplified by spectroscopic biosensors [ 49 , 64 , 65 , 66 , 67 , 68 , 69 , 99 ]. Other than the widely used offline ISCUSflex colorimetric bedside analyser, all optical based techniques integrated with microdialysis to quantify TBI metabolites have utilised infrared spectroscopic sensors.…”

Section: Review Of Sensor Technologies For Brain Metabolismmentioning

confidence: 99%

“…The versatility of optical sensing technologies integrated with microdialysis for reagent-free multi-analyte assays has been demonstrated by various studies [ 49 , 64 , 65 , 66 , 67 , 68 , 69 , 99 ]. Examples are presented in Figure 7 [ 49 , 69 ].…”

Section: Review Of Sensor Technologies For Brain Metabolismmentioning

confidence: 99%

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Monitoring Neurochemistry in Traumatic Brain Injury Patients Using Microdialysis Integrated with Biosensors: A Review

et al. 2022

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In a traumatically injured brain, the cerebral microdialysis technique allows continuous sampling of fluid from the brain’s extracellular space. The retrieved brain fluid contains useful metabolites that indicate the brain’s energy state. Assessment of these metabolites along with other parameters, such as intracranial pressure, brain tissue oxygenation, and cerebral perfusion pressure, may help inform clinical decision making, guide medical treatments, and aid in the prognostication of patient outcomes. Currently, brain metabolites are assayed on bedside analysers and results can only be achieved hourly. This is a major drawback because critical information within each hour is lost. To address this, recent advances have focussed on developing biosensing techniques for integration with microdialysis to achieve continuous online monitoring. In this review, we discuss progress in this field, focusing on various types of sensing devices and their ability to quantify specific cerebral metabolites at clinically relevant concentrations. Important points that require further investigation are highlighted, and comments on future perspectives are provided.

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Section: Review Of Sensor Technologies For Brain Metabolismmentioning

confidence: 99%

Section: Review Of Sensor Technologies For Brain Metabolismmentioning

confidence: 99%

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Monitoring Neurochemistry in Traumatic Brain Injury Patients Using Microdialysis Integrated with Biosensors: A Review

et al. 2022

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“…Due to hydrogen bonding effects between water and glycerol, CLS methods need additional attentiveness within the spectral region around 1000 cm −1 , which will be discussed in the following section. Another option is certainly the application of partial least squares (PLS) analysis, which requires formulated samples with varying concentrations of all constituents for calibration [22,30,31]. An advantage, compared with the CLS method, is the inclusion of compounds with pH-dependent vibrational bands, e.g., as experienced for the inorganic phosphate spectra.…”

Section: Quantification Of Insulin and Excipients In Formulationsmentioning

confidence: 99%

FT-IR versus EC-QCL spectroscopy for biopharmaceutical quality assessment with focus on insulin—total protein assay and secondary structure analysis using attenuated total reflection

Delbeck

Heise

2020

Anal Bioanal Chem

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For the quality control of biopharmaceutical products, which contain proteins as the most important active ingredients, shelf life may be limited due to inappropriate storage conditions or mechanical stress. For insulins as representatives of life-saving pharmaceuticals, analytical methods are needed, which are providing additional information than obtained by assays for total protein quantification. Despite sophisticated formulations, the chemical stability may be challenged by temperatures deviating from recommended conditions or shear rate exposure under storage, leading to misfolding, nucleation, and subsequent fibril formation, accompanied by a decrease in bioactivity. A reliable method for insulin quantification and determination of secondary structure changes has been developed by attenuated total reflection (ATR) Fourier-transform infrared spectroscopy of insulin formulations by a silver halide fiber-coupled diamond probe with subsequent dry-film preparation. A special emphasis has been placed on the protein amide I band evaluation, for which spectral band analysis provides unique information on secondary structure fractions for intact and misfolded insulins. Quantitative measurements are possible down to concentrations of less than 0.5 mg/ml, whereas the dry-film preparation delivers high signal-to-noise ratios due to the prior water evaporation, thus allowing a reliable determination of secondary structure information.

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“…Several research groups have worked with systems for biomedical laser spectroscopy in the mid-infrared following the commerical availability of EC-QCLs. These systems have mainly used either free-space configurations with liquid samples in transmission cells, 2,3 or fiber-coupled setups with either transmission, reflectance, or attenuated total reflection (ATR) sensing modalities. [4][5][6][7] Fiber-coupled setups are more practical for portable sensor development, while free-space setups can potentially be used for larger systems in e.g.…”

Section: Introductionmentioning

confidence: 99%

Mid-infrared spectroscopy with a fiber-coupled tuneable quantum cascade laser for glucose sensing

Jernelv

Strøm

Hjelme

et al. 2020

Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX

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A fiber-coupled transmission spectroscopy setup using a pulsed external-cavity quantum cascade laser (EC-QCL, 1200-900 cm −1 ) has been developed and demonstrated for measurements of aqueous solutions. The system has been characterised with regard to the laser noise and optimal optical pathlength. Solutions with glucose were used to further test the setup, and glucose concentrations down to physiologically relevant levels (0-600 mg/dL) were investigated. Albumin, lactate, urea, and fructose in various concentrations were added as interfering substances as their absorption bands overlap with those of glucose, and because they may be of interest in a clinical setting. Analyte concentrations were predicted using partial least-squares (PLS) regression, and the root-mean-square error of cross-validation for glucose was 10.7 mg/dL. The advantages of using a convolutional neural network (CNN) for regression analysis in comparison to the PLS regression were also shown. The application of a CNN gave an improved prediction error (8.3 mg/dL), and was used to identify important spectral regions. These results are comparable to state-of-the-art enzymatic glucose sensors, and are encouraging for further research on optics-based glucose sensors.

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Reliable glucose monitoring by ex-vivo blood microdialysis and infrared spectrometry for patients in critical care

Cited by 3 publications

References 22 publications

Monitoring Neurochemistry in Traumatic Brain Injury Patients Using Microdialysis Integrated with Biosensors: A Review

Monitoring Neurochemistry in Traumatic Brain Injury Patients Using Microdialysis Integrated with Biosensors: A Review

FT-IR versus EC-QCL spectroscopy for biopharmaceutical quality assessment with focus on insulin—total protein assay and secondary structure analysis using attenuated total reflection

Mid-infrared spectroscopy with a fiber-coupled tuneable quantum cascade laser for glucose sensing

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