Reliability of plant root comet assay in comparison with human leukocyte comet assay for assessment environmental genotoxic agents

Reis, Gabriela Barreto dos; Andrade-Vieira, Larissa Fonseca; Moraes, Isabella de Campos; César, Pedro Henrique Souza; Marcussi, Silvana; Davide, Lisete Chamma

doi:10.1016/j.ecoenv.2017.04.004

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…In an ecotoxicological context, plant systems can provide relevant information for a picture of the toxicological footprint of several compounds. Additionally, as discussed earlier, plant-based toxicity assays have widely been used as alternative models, with comparable outcomes to those obtained in animal studies [25]. However, relatively few studies (n=988; PubMed search string "Nanoparticle toxicity" and "Plants") have addressed toxicity in plants.…”

Section: Study Selectionmentioning

confidence: 99%

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Genotoxicity of engineered nanoparticles in higher plants

Ghosh

Godderis

et al. 2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Nanoparticles (NPs) are an emerging environmental threat. However, studies of NPs in different environmental components are limited. In this review, we discuss studies that have evaluated the genotoxicity of NPs in higher plants. Among the studies reviewed, silver NPs were most studied (n=7 articles), with fewer studies reporting the genotoxicity of carbon nanotubes (n=3), titanium dioxide NPs (n=4), and zinc oxide NPs (n=3). Most of the genotoxicity studies were performed in the model plant systems Allium sp (n=22), Nicotiana sp (n=4) and Vicia sp (n=4) using chromosome aberration (n=22), micronucleus (n=15) and comet assays (n=14). Genotoxicity was observed in most of the studies; however, many studies did consider key determinants of NP toxicity such as particle characterization, dissolution, and uptake. From this review, we propose a set of guidelines that should be considered when reporting results of NP toxicity in plants.

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Section: Study Selectionmentioning

confidence: 99%

“…Reference images for different kinds of aberrations, which may be used for proper scoring, are in the review by Leme and Marin-Morales [10] and could be used. For comet assay, 25…”

Section: <Figure 3>mentioning

confidence: 99%

Genotoxicity of engineered nanoparticles in higher plants

Ghosh

Godderis

et al. 2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Due to its wide variety of applications, comet assay is utilized by many researchers to assess DNA damage in the workers of different industries. During the literature review, a number of health biomonitoring studies were found that used comet assay (12)(13)(14)(15)(16). Those studies were aimed at assessing the amount of genotoxicity caused by particular exposures in the workers of different industries, such as open-cast coal mining (17), the automotive industry (18), lead battery recycling plants (19), and the petrol industry (20).…”

Section: Resultsmentioning

confidence: 99%

Comet assay as a sensitive technique in occupational health studies; A literature review

Singh

2018

JOHE

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Article InfoBackground: Occupational health is a sensitive field in many countries where occupational exposure needs attention due to the lack of awareness. Several businesses, including leather, textile, iron, steel, and dyeing industries use a variety of mutagenic and genotoxic chemicals. Exposure to these chemicals may pose serious health risks, including genetic damage to workers. Comet assay is a technique that has been extensively used in assessing DNA damage in such workers. Materials and methods: The literature search was done using PubMed and Google Scholar databases. Some studies were found reporting the use of comet assay as a sensitive and useful technique in assessing genetic damage in occupationally exposed workers. These studies have been presented in this paper to emphasize the importance and propriety of comet assay in assessing the genetic impact of hazardous exposures. Result: Having gone through the available reports, the author of this paper found comet assay as a useful biotechnological technique in the field of occupational biomonitoring. This technique is a sensitive and cheap method for assessing genotoxicity. Conclusion: Comet assay can be used as a reliable technique to quantify the impact of genotoxicants in the form of DNA damage at a single-cell level. This assay can be considered as a sensitive parameter whenever health assessment studies are to be designed.

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“…However, the issues of reliability of plant chromosome aberration bioassays and the possibility of results extrapolation to humans are still questioned by some scientists. Comparison studies between comet assay using plant root cells and human leukocytes demonstrated a correlation between results obtained for plant cells and human cells, proving that DNA damage is universal, independently of the physiology of the organism itself (Reis et al, 2017).…”

Section: Genotoxicity Assessment Using Triticum Aestivum Assaymentioning

confidence: 86%

“…Furthermore, the Gene-Tox program (launched by the US Office of Toxic Substances and the US Environmental Protection Agency) concluded that higher plants are a suitable tool for the detection of genotoxic contaminants (Lanier et al, 2015). To support this statement, several studies have proven the reliability of plant assay (Juchimiuk et al, 2006;Lanier et al, 2015;Palmieri et al, 2016;Reis et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Triticum aestivum Assay - A Useful Tool for Environmental Monitoring and Toxicity Assessment

Jităreanu

Caba

Trifan

et al. 2019

Not Bot Horti Agrobo

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The present review summarizes the literature data regarding the application of Triticum aestivum assay as an alternative method for toxicity assessment of environmental pollutants or potential therapeutic agents. Plant bioassays present several advantages among other biological assays (simplicity, low cost, rapid test activation, a wide array of assessment endpoints). They present a good correlation with animal and human cells models, and are a reliable tool for genotoxicity assessment. Furthermore, in the context of toxicology guidelines that promote the substitution of assays using animal models with other bioassays, genotoxicity assays using higher plants models have gained in popularity. The present review focuses on three major aspects regarding Triticum aestivum assay - its utility in environmental pollution monitoring, its application in genotoxicity assessment studies, and its application in phytotoxicity evaluation of nanomaterials. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Reliability of plant root comet assay in comparison with human leukocyte comet assay for assessment environmental genotoxic agents

Cited by 32 publications

References 34 publications

Genotoxicity of engineered nanoparticles in higher plants

Genotoxicity of engineered nanoparticles in higher plants

Comet assay as a sensitive technique in occupational health studies; A literature review

Triticum aestivum Assay - A Useful Tool for Environmental Monitoring and Toxicity Assessment

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