Reliability of Parameters of Complete Blood Count With Different Storage Conditions

Abstract

This study shows that most parameters of the CBC are unaffected with the studied storage temperature up to 48 hr except for the PLT which should be performed within 6 hr of the post-collection time. To avoid changes in a few parameters such as Htc, it is best to store the sample at 4 ± 2°C if any delay is anticipated.

Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

“…Our result of HCT was stable up to 54 h that is similar to other studies at 4°C [6,15,17,19,20]. Result of MCV was found to be stable up to 42 h, regarding result for MCV at 4°C, previous studies are also show supporting with our findings [5,6,15,20,21].…”

“…Result of MCV was found to be stable up to 42 h, regarding result for MCV at 4°C, previous studies are also show supporting with our findings [5,6,15,20,21]. Studies by Turhan et al [5] and Lippi et al [2] demonstrated increment was observed on the second day due to reflect the swelling of RBC at room temperature.…”

“…the lifespan (t1/2) of cold-and RT-stored platelets are 1.3 and 3.9 days, respectively [26][27][28]. Concluded by Massimo Daves et al Platelet count using (impedance & fluorescent technique) is stable at 4°C up to 6 h and Dammika Gunawardena et al is also concluded PLT and MPV was stable up to 6 h [6,4]. Increment in MPV over time reveal by many studies [5,17,20,21,29].…”

“…Influence of storage and variability on the majority of the blood count parameters can be minimized and if the analysis takes place before 24 h at 4°C [6,9,12,18]. …”

“…The condition is worst in tropical countries like Pakistan where humidity and high temperature are at the peak. It is therefore very important to know to save the stability of samples in conditions existing in tropical areas [5,6]. Running prestored blood samples is very frequent in our laboratories.…”