Reliability of enzymatic lysozyme determinations.

Tepper, T.; Meijer, Simen; Brouwer-Pijpen, G

doi:10.1136/jcp.29.2.170

J Clin Pathol

1976

DOI: 10.1136/jcp.29.2.170

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Reliability of enzymatic lysozyme determinations.

T. Tepper¹,

Simen Meijer²,

G Brouwer-Pijpen³

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1978

1984

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Cited by 2 publications

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“…However, the validity of using the generated experimental data for diagnostic purposes has sometimes been complicated by reports by different investigators of the presence of widely different lysozyme levels for the same biological source. As has been pointed out by many investigators, differences in reported lysozyme concentrations may arise because of the assay systems used to quantitate the presence of the enzyme in the biological fluids (6,7,15,19,21,24). There are several problems, including the use of a hen egg-white lysozyme standard instead of purified human lysozyme, which gives significantly higher values of unknown enzyme concentrations and is further complicated by the variability noted among the different assay systems (8).…”

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confidence: 99%

Development of an enzyme-linked immunosorbent assay for determination of lysozyme in human parotid and submandibular-sublingual salivas

Mackay¹,

Goodman²,

Cox³

et al. 1984

J Clin Microbiol

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The specificity of lysozyme determinations in human parotid and submandibular-sublingual salivas of two subjects was assessed by comparison of lysozyme concentrations in native acidified salivas with purified enzyme obtained by immunoadsorbent fractionation of the salivas. Lysozyme concentrations were measured by the turbidimetric catalytic method and by a newly developed enzyme-linked immunosorbent assay (ELISA). The validity of the assays was established by comparing assay results with enzyme concentration values determined from optical density-extinction coefficient calculations of the purified lysozyme peak. Values for purified enzyme were found to be similar, irrespective of the assay used to determine lysozyme concentrations, and were in agreement with extinction coefficient calculations. Based on the ELISA technique, recoveries of lysozyme from both parotid and submandibular-sublingual salivas were greater than 75 and 90%, respectively. Similar recoveries were noted for parotid saliva when determinations were based on the turbidimetric assay. However, the ELISA and turbidimetric assays differed with respect to lysozyme levels in submandibular-sublingual saliva because of the apparent presence of an enhancement factor which gave rise to higher lysozyme values in the catalytic assay and therefore resulted in low recoveries of purified enzyme. This catalytic enhancement factor was present in the nonadsorbed fraction of both subjects, as higher lysozyme activities were noted when nonadsorbed fractions were added to affinity-purified lysozymes. Lysozyme levels were also determined in the parotid and submandibular-sublingual salivas of caries-resistant and -susceptible adults. In general, levels of lysozyme in parotid saliva were lower in comparison to submandibular -sublingual saliva; however, significant differences in enzyme concentration were not evident between the caries-resistant and caries-susceptible subjects.

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mentioning

confidence: 99%

Development of an enzyme-linked immunosorbent assay for determination of lysozyme in human parotid and submandibular-sublingual salivas

Mackay¹,

Goodman²,

Cox³

et al. 1984

J Clin Microbiol

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Zur Rolle des Lysozyms bei der Mineralisation von Callusgewebe

Siebert

Treber

Konold

et al. 1978

Langenbecks Arch Chiv

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Investigations have suggested that lysozyme (E.C. 3.2.1.17) is involved in bone mineralization. High concentrations of lysozyme is found in the growth plate near cartilage bone junction, where it is located at the collagen fibrils and in the ground substance. Quantitative studies of lysozyme levels were made in ossifying tissue of healing fractures, to confirm the existence of this relationship on bone repair. Callous tissue, serum samples and normal bone was collected from 42 rats at 15 intervalls during a 50 day healing period. Agar gel diffusion test was used for quantitation of lysozyme. Electrophoresis of tissue extract and standard henn egg white lysozyme served as control. Lysozyme levels in callous tissue increased significantly (4--5-fold) from 4.--21. day p. trauma and subsequently decreased. The concentration in serum samples did not change significantly. Changes in Ca concentration and histological studies during tests confirm a direct relationship between bone mineralization and lysozyme level changes.

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Reliability of enzymatic lysozyme determinations.

Cited by 2 publications

References 7 publications

Development of an enzyme-linked immunosorbent assay for determination of lysozyme in human parotid and submandibular-sublingual salivas

Development of an enzyme-linked immunosorbent assay for determination of lysozyme in human parotid and submandibular-sublingual salivas

Zur Rolle des Lysozyms bei der Mineralisation von Callusgewebe

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