Reliability of Automated Biochemical Identification of Burkholderia pseudomallei Is Regionally Dependent

Podin, Yuwana; Kaestli, Mirjam; McMahon, Nicole; Hennessy, Jann; Ngian, Hie Ung; Wong, Jin Shyan; Mohana, Anand; Wong, See Chang; William, Timothy; Mayo, Mark; Baird, Robert W.; Currie, Bart J.

doi:10.1128/jcm.01290-13

Cited by 28 publications

(21 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…pseudomallei readily grows in commercially available blood culture media, but it is not unusual for laboratories in nonendemic locations to misidentify the bacterium as a Pseudomonas or other Burkholderia species or to discount it as a contaminant, with some commercial identification systems being poor at identifying B. pseudomallei. 115,116 Until they develop more comprehensive profile databases, caution is also required with identification of B. pseudomallei using the evolving matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems. 117 Urine cultures and cultures from nonsterile sites increase the likelihood of diagnosis; the rate of successful diagnosis is increased if sputum, ulcer or skin lesion swabs, throat and rectal swabs are placed into Ashdown broth, a colistin-containing liquid transport medium that facilitates the selective growth of B. pseudomallei, or directly plated onto Ashdown agar which contains gentamicin or a commercial B. cepacia medium.…”

Section: Diagnosis: Culture Of B Pseudomallei Remains the Gold Standardmentioning

confidence: 99%

Melioidosis: Evolving Concepts in Epidemiology, Pathogenesis, and Treatment

Currie

2015

Semin Respir Crit Care Med

265

347

View full text Add to dashboard Cite

Infection with Burkholderia pseudomallei can result in asymptomatic seroconversion, a single skin lesion that may or may not heal spontaneously, a pneumonia which can be subacute or chronic and mimic tuberculosis or rapidly progressive resulting in fatal overwhelming sepsis. Latency with subsequent activation of disease is well recognized, but very uncommon. Melioidosis also has a myriad of other clinical presentations and diagnosis is often delayed because of this and because of difficulties with laboratory diagnosis and lack of recognition outside melioidosis-endemic regions. The perception of B. pseudomallei as a top tier biothreat agent has driven large funding for research, yet resources for diagnosis and therapy of melioidosis in many endemic locations remain extremely limited, with mortality as high as 50% in comparison to around 10% in regions where state-of-the-art intensive care therapy for sepsis is available. Fatal melioidosis is extremely unlikely from natural infection in a healthy person, provided the diagnosis is made early, ceftazidime or meropenem is commenced and intensive care therapy is available. While biothreat research is directed toward potential aerosol exposure to B. pseudomallei, the overall proportion of melioidosis cases resulting from inhalation rather than from percutaneous inoculation remains entirely uncertain, although the epidemiology supports a shift to inhalation during severe weather events such as cyclones and typhoons. What makes B. pseudomallei such a dangerous organism for patients with diabetes and other selective risk factors remains unclear, but microbial genome-wide association studies linking clinical aspects of melioidosis cases to nonubiquitous or polymorphic B. pseudomallei genes or genomic islands are beginning to uncover specific virulence signatures. Finally, what also remains uncertain is the global phylogeography of B. pseudomallei and whether melioidosis is spreading beyond historical locations or is just being unmasked in Africa and the Americas by better recognition and increased surveillance.

show abstract

Section: Diagnosis: Culture Of B Pseudomallei Remains the Gold Standardmentioning

confidence: 99%

Melioidosis: Evolving Concepts in Epidemiology, Pathogenesis, and Treatment

Currie

2015

Semin Respir Crit Care Med

265

347

View full text Add to dashboard Cite

show abstract

“…The misidentification of B. pseudomallei as B. cepacia and vice versa by commonly used Vitek 2 system was repeatedly described (3,9,10). Recently, Podin et al noted that two enzymatic tests, NAGA (beta-N-acetyl-galactosaminidase) and BNAG (beta-N-acetyl-glucosaminidase), were distinct between correctly and misidentified B. pseudomallei isolates (11). In addition, the combinations of positive dCEL (Dcellobiose) and negative ProA (L-proline arylamidase), TyrA (tyrosine arylamidase), or NAGA were observed in B. pseudomallei strains incorrectly identified as B. cepacia (12).…”

Section: Discussionmentioning

confidence: 99%

The Complexity of the Identification of Burkholderia cepacia Strain Which Caused Septicemia

Bui

Zakharova

Шпак

et al. 2018

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: The correct identifying of pathogenic Burkholderia spp. using available commercial biochemical systems is a certain problem due to metabolic plasticity and variable enzymatic profile of isolates. Objectives: The current study aimed at using specific PCR and conventional multi-locus sequence typing (MLST) scheme to confirm the uncertain identification results for Burkholderia cepacia clinical isolate. Methods: Multilocus species-specific PCR and MLST profiling of high-throughput sequencing data have been used to clarify the varied results of biochemical identification of the strain. Results: The strain isolated from a patient with septicemia was initially identified as B. pseudomallei by Vitek 2 GN system but has an uncharacteristic antimicrobial resistance pattern and colony morphology. A species-specific multilocus PCR and whole-genome sequence profiling, according to the MLST scheme, allowed to identify an isolate as B. cepacia. Conclusions: The obtained results demonstrate the preference of molecular tests for correct identifying of pathogenic Burkholderia, considering that misidentification of those may lead to improper treatment or increase of biosafety risk.

show abstract

“…Currently, the methods of detecting B.pseudomallei primarily include biochemical identification, molecular biology methods and mass spectrometry. Rapid biochemical analysis systems, such as API 20NE, Vitek 1 and Vitek 2 database, all contain B.pseudomallei,but it takes about 6h-18 h, and the correct identification rate is unstable(accuracy was 53%-98%) [5].…”

mentioning

confidence: 99%

Research on the establishment and application of protein fingerprint spectrum database of Burkholderia pseudomallei in Hainan Province China

Wang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The aim of this study was to establish a SuperSpectrum of Burkholderia pseudomallei(B. pseudomallei) in Hainan and evaluate its application value in the rapid identification of clinical isolates of B. pseudomallei.Methods: Using a collection of 167 isolates of B. pseudomallei from June 2010 to May 2019 in different regions of Hainan province, multilocus sequence typing (MLST) was performed, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for spectrum acquisition. A SuperSpectrum was created based on the selection of 80 representative average spectra. In a second step, we validated the SuperSpectra with 137 strains of B. pseudomallei, 8 strains of Burkholderia thailandensis(B. thailandensis), 2 strains of Burkholderia cepacia(B. cepacia), 1 strain of Burkholderia cenocepacia(B. cenocepacia) and 1 strain of Burkholderia multivorans(B. multivorans), as well as 1 strain of Burkholderia gladioli(B. gladioli) identified by MLST typing and 16S rRNA gene sequencing.Results: The results showed that there was 100% agreement between the validation strains analyzed by MALDI-TOF MS and those evaluated by MLST typing and 16S rRNA gene-sequencing analysis methods. Protein fingerprints spectra showed that specific peaks occurred in B. pseudomallei from the Hainan region. The result of clustering typing indicated that B. pseudomallei and its closely related species could be well classified by MALDI-TOF MS at the protein level.Conclusions: MALDI-TOF MS is a promising, rapid, and economical method to monitor the outbreaks and spread of B. pseudomallei isolates.The establishment of an accurate and objective SuperSpectrum database can provide a new platform for the clinical rapid diagnosis of melioidosis.

show abstract

Reliability of Automated Biochemical Identification of Burkholderia pseudomallei Is Regionally Dependent

Cited by 28 publications

References 22 publications

Melioidosis: Evolving Concepts in Epidemiology, Pathogenesis, and Treatment

Melioidosis: Evolving Concepts in Epidemiology, Pathogenesis, and Treatment

The Complexity of the Identification of Burkholderia cepacia Strain Which Caused Septicemia

Research on the establishment and application of protein fingerprint spectrum database of Burkholderia pseudomallei in Hainan Province China

Contact Info

Product

Resources

About