Reliability of acid-insoluble ash as internal marker for the measurement of digestibility in rabbits

Papadomichelakis, George; Fegeros, K.

doi:10.4995/wrs.2020.12216

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…With respect to using rabbits in digestion studies, these results caution against the use of acid insoluble ash as a digestibility marker (Papadomichelakis & Fegeros, 2020) in animals that had previous access to soil or soil-contaminated diets, or against diets that contain substances that might be sequestered by the 'wash-back' colonic separation mechanism. Nevertheless, the observation that the vast majority of the 14-day silica intake (app.…”

Section: Discussionmentioning

confidence: 98%

Sand accumulation in the digestive tract of rabbits (Oryctolagus cuniculus) and guinea pigs (Cavia porcellus): The role of the appendix

Winter

Clauss

Codron

et al. 2021

Journal of Morphology

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We determined location and amount of accumulated sand in the gastrointestinal tract (GIT) of rabbits (Oryctolagus cuniculus) and guinea pigs (Cavia porcellus) fed diets containing external (silicate) abrasives. Computed tomographic abdominal images of rabbits (n = 44) and guinea pigs (n = 16) that each received varying numbers (4-7) of different diets for 14 days each (total n = 311 computed tomographs), and radiographs of dissected GIT and presence of silica in GIT content (n = 46 animals) were evaluated. In rabbits, the majority of accumulated sand was located in the caecal appendix, an elongated, intestinal structure in the left side of the abdomen. The 'wash-back' colonic separation mechanism in rabbits may be partly responsible for a retrograde transport of sand back to the caecum, where dense, small particles accumulate in the appendix. The appendix likely acted as a reservoir of these particles, leading to significant effects not only of the momentary but also of the previous diet on recorded sand volumes in the rabbits. Guinea pigs have no caecal appendix and a colonic separation mechanism not based on a 'wash-back'. Less sand accumulation was found in their GIT without a specific location pattern, and there were less previous diet effects in this species. None of the rabbits or guinea pigs developed clinical signs of obstruction during the study, and the recorded sand volumes represented 1.0 ± 1.2% of the 14-d sand intake in rabbits and 0.2 ± 0.2% in guinea pigs. Accumulation of sand in volumes up to 10 cm 3 in the GIT of rabbits does not seem to cause clinical health impairment. Large inter-individual differences in rabbits indicate interindividual variation in proneness to sand accumulation. The reason for the presence of a sand-trapping caecal appendix in animals that are, due to their burrowing lifestyle and feeding close to the ground, predestined for accidental sand ingestion, remains to be unveiled.

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Section: Discussionmentioning

confidence: 98%

Sand accumulation in the digestive tract of rabbits (Oryctolagus cuniculus) and guinea pigs (Cavia porcellus): The role of the appendix

Winter

Clauss

Codron

et al. 2021

Journal of Morphology

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“…Later, laboratory DM was estimated by keeping powdered faecal samples at 105 • C for 3 h according to AFIA [28] protocol. The total tract apparent DM and OM digestibility were determined using the acid insoluble ash [29] technique developed by Van Keulen and Young [30] with slight modifications [31,32]. The NDF [5] and ADF were determined according to the method described by ANKOM technology using an ANKOM2000 Fiber Analyzer (ANKOM Technology, New York, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

Effects of Feeding a Commercial Starch Binding Agent during Heat Stress on Enteric Methane Emission, Rumen Volatile Fatty Acid Contents, and Diet Digestibility of Merino Lambs

et al. 2023

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Twenty-four Merino lambs were allocated to three dietary treatment groups to determine the effects of a dietary starch and protein binding agent and heat stress on methane (CH4) emissions and rumen parameters. The diets were a wheat-based diet (WD), a 2% Bioprotect™ (Bioprotect™, RealisticAgri, Rutland, UK) treated wheat-based diet (BD), and a maize-based diet (MD) for 3 periods of 1-week duration. During Period 1 (P1) the lambs were maintained under thermoneutral conditions and at a 1.7 × Maintenance (M) level. During P2 and P3, the lambs were maintained under cyclic heat stress conditions and fed at 1.7 × M and 2.0 × M, respectively. Total CH4 production was lower for the BD diet than the WD diet, which in turn was lower than the MD diet (p < 0.001). Total CH4 production was lower during P2 than P1 with P3 intermediate (p = 0.04). Rumen total volatile fatty acid (TVFA) concentrations were higher for the WD diet than the MD diet with the BD diet intermediate (p = 0.01). Rumen TVFA concentrations were lower during heat stress than under thermoneutral conditions (p < 0.001). Whole tract starch digestibility was higher for the BD and WD diets than the MD diet (p < 0.001). In conclusion, feeding Merino lambs the BD diet reduces CH4 emissions without reducing starch digestibility.

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“…Acid insoluble ash could be used as an internal marker to calculate the digestibility of a diet precisely, and the method could be used as an alternative to total collection method in many animals (Figueiredo et al, 2019;Papadomichelakis & Fegeros, 2020). However, Prawirodigdo, Gannon, Leury and Dunshea (2021) demonstrated that the basal diet and choice of indigestible marker can substantially influence the determination of apparent total tract digestibility and that the use of AIA as an indigestible marker varies with species of animal used.…”

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confidence: 99%

Sources of acid insoluble ash as marker of corn digestibility in broilers

Silva

Barbosa

Carvalho

et al. 2022

Semina: Ciênc. Agrár.

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The objective was to evaluate three sources of acid insoluble ash (AIA) (celite, kaolin, and sand), as indicators, to determine the apparent metabolizable energy of corn on a natural matter (AME) basis, dry matter digestibility coefficient (DMDC), and crude protein digestibility coefficient (CPDC) using total and partial excreta collection methods. Two hundred and ten Ross broiler chicks of 18 to 27 days of age were used. Broilers were adapted to experimental the diet for four days, and excreta were collected for 5 days. A reference diet (RD) based on corn and soybean meal was prepared to meet the nutritional requirements of the birds. A test diet was prepared with 40% replacement of RD with corn. The formulated diets were: D1, 99% Reference diet + 1% celite; D2, 59.4% RD + 39.6 % corn + 1% celite; D3, 99% Reference diet + 1% kaolin; D4, 59.4% RD + 39.6 % corn + 1% kaolin; D5, 99% Reference diet + 1% sand; D6, 59.4% RD + 39.6 % corn + 1% sand. The treatments were distributed in a completely randomized design, with six diets and five replicates of seven birds each in a 2x3 factorial arrangement. There were significant interactions for all variables investigated. It was observed that the AME and DMDC values of corn were similar in the two methods of collection, and PDC values using kaolin and celite makers were similar. However, the AME and CPDC of corn using sand with partial collection method were underestimated by 17.70 and 15.53%, respectively compared to those with the total collection method. The AME values of corn with the collection methods using celite and, the DMDC using celite and sand were significantly different. It was observed that the DMDC using celite and sand provided significantly lower values (4.67 and 5.15%), respectively, and the AME using celite was 2.86% lower than that obtained by total collection. To determine the EMA, DMDC, and CPDC in broilers, it is more efficient to use celiteTM and kaolin as markers with partial collection of excreta. Sand should not be used with partial excreta collection method, because it provides lower values of AME and CPDC compared to with total excreta collection method.

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Reliability of acid-insoluble ash as internal marker for the measurement of digestibility in rabbits

Cited by 4 publications

References 32 publications

Sand accumulation in the digestive tract of rabbits (Oryctolagus cuniculus) and guinea pigs (Cavia porcellus): The role of the appendix

Sand accumulation in the digestive tract of rabbits (Oryctolagus cuniculus) and guinea pigs (Cavia porcellus): The role of the appendix

Effects of Feeding a Commercial Starch Binding Agent during Heat Stress on Enteric Methane Emission, Rumen Volatile Fatty Acid Contents, and Diet Digestibility of Merino Lambs

Sources of acid insoluble ash as marker of corn digestibility in broilers

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