Reliability in Automatic Determination of the ABO Group by the Groupamatic System

Abstract

The reliability of the automatic determination of the ABO group on the Groupamatic is explained and backed up with an experience of 600,000 runs. Provided the user observes the rules of conventional serology, this reliability depends upon three essential, combined actions of the machine: identifying the sample, reading serological reactions, processing and printing-out grouping data. Figures taken from 150,000 ABO groups enable us to compare the frequency of detected weak A sub-groups with known properties ; tests performed on samples of chimeras or of Bombay phenotypes, associated with experimental work on artificial chimeras, confirm the high reliability this automatic system provides.

Red Cell Antibody Screening with the Groupamatic System

“…The principle of the Groupamatic system and its mode of operation have already been published [7,8,[10][11][12]19]. The blood samples (from volun tary donors) to be tested are collected in vacuum tubes containing EDTA (Becton Dickinson; 0.06 ml of a 15% solution in a 7-ml tube).…”

ABO, H, and Lewis Systems

“…The frequency has been estimated as 1 per 1000 group A Danes [274] , 9 in 150 000 French donors (0.0136% of group A) [275] , and 2 in about 180 000 Canadians [276] . The frequency has been estimated as 1 per 1000 group A Danes [274] , 9 in 150 000 French donors (0.0136% of group A) [275] , and 2 in about 180 000 Canadians [276] .…”

Groupamatic System: Overview, History of Development and Evaluation of Use

“…ABO, D, Du typing and Rh phenotyping are very easy and reliable [1,19,24,27,37,41,43]. For Kell and Le(a) typing, the quality of the reaction depends upon selection of appropriate antisera [6,17,26,42], Provided that appropriate antisera are selected, deter mination of these antigens is as reliable as the determination of the other antigens.…”