Relevant biological processes for tissue development with stem cells and their mechanistic modeling: A review

Paim, Ágata; Cardozo, Nilo Sérgio Medeiros; Tessaro, Isabel Cristina; Pranke, Patrícia

doi:10.1016/j.mbs.2018.05.007

Cited by 6 publications

(4 citation statements)

References 175 publications

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“…We observed that treatment with 5% PL for 24 h increased rMSCs migration by almost 47% (1 and 1.47 + 0.04 for control and 5% PL, respectively; n = 5, p < 0.0001) (Figures 2A,B) suggesting that PL contains factors that stimulate this process. Migration of MSCs is modulated by external cues such as cytokines, chemokines, and growth factors, which regulate several cellular processes (polymerization of actin filaments, cell polarization, and cell membrane protrusion activity) (Paim et al, 2018). Previous studies have shown that PL is rich in growth factors such as PDGF, FGF, and transforming growth factor (TGF) (Mazzucco et al, 2010;Hayon et al, 2013) that can modulate migration by mechanisms involving ion channel activation.…”

Section: Bk Channels Are Involved In the Effect Of Pl On Rmscs Migrationmentioning

confidence: 99%

Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

et al. 2020

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Due to their capacity to proliferate, migrate, and differentiate, mesenchymal stem cells (MSCs) are considered to be good candidates for regenerative medicine applications. The mechanisms underlying proliferation and differentiation of MSCs have been studied. However, much less is known about the mechanisms regulating the migration of MSCs. Platelet lysate (PL), a supplement used to promote cell expansion, has been shown to promote MSCs migration; however, the underlying mechanism are unknown. Here, by using adipose-derived rat MSCs (rMSCs) and the scratch assay in the absence and presence of various BK channels modulators, we evaluated the role of BK channels in mediating the PL-stimulated migration of rMSCs. We found that 5% PL increased rMSCs migration, and this effect was blocked by the addition of the BK channel selective antagonist Iberiotoxin (IBTX). In the absence of PL, the BK channel agonist NS1619, stimulated rMSCs migration to similar level as 5% PL. Addition of both NS1619 and 5% PL resulted in an increase in rMSCs migration, that was higher than when either one was added individually. From whole-cell recordings, it was found that the addition of 5% PL increased the magnitude of BK current density. By using Western blot and flow cytometry, it was found that PL did not affect the expression of BK channels. Together, our results indicate that as shown in other cell types, activation of BK channels by themselves also promote rMSC migration, and show that activation of BK channels contribute to the observed PL-induced increase in migration of rMSC.

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Section: Bk Channels Are Involved In the Effect Of Pl On Rmscs Migrationmentioning

confidence: 99%

Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

et al. 2020

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“…The computational estimation studies of shear stress are complex and involve interactions between the matrices, cells, and the culture system of tissue development in vitro. The models to predict tissue development have limitations and are challenging [ 40 ]. In a study of computational fluid dynamics focusing on the effect of the flow rate, the high flow (20, 40, and 60 mL/min) increased the shear stress on the scaffold structure.…”

Section: Discussionmentioning

confidence: 99%

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

2023

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Decellularization of organs creates an acellular scaffold, ideal for being repopulated by cells. In this work, a low-cost perfusion system was created to be used in the process of liver decellularization and as a bioreactor after recellularization. It consists of a glass chamber to house the organ coupled to a peristaltic pump to promote liquid flow through the organ vascular tree. The rats’ liver decellularization was made with a solution of sodium dodecyl sulfate. The recellularization was made with 108 mesenchymal stromal/stem cells and cultivated for seven days. The decellularized matrices showed an absence of DNA while preserving the collagen and glycosaminoglycans quantities, confirming the efficiency of the process. The functional analyses showed a rise in lactate dehydrogenase levels occurring in the first days of the cultivation, suggesting that there is cell death in this period, which stabilized on the seventh day. Histological analysis showed conservation of the collagen web and some groups of cells next to the vessels. It was possible to establish a system for decellularization and a bioreactor to use for the recellularization method. It is easy to assemble, can be ready to use in little time and be easily sterilized.

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“…They can be classified into embryonic stem cells (ESC), fetal stem cells (FSC) and adult stem cells [ 1 ]. ESCs are obtained from embryos and can differentiate spontaneously, making the control of their differentiation challenging; they also have the potential to generate teratomas, and due to their origin, there are ethical barriers that hinder their use in regenerative medicine [ 2 , 3 ]. FSCs can be isolated from the fetus, placenta, amniotic fluid and umbilical cord, and they are more plastic than adult stem cells [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins

Oliveira¹,

Irioda²,

Stricker³

et al. 2021

Membranes

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Adipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that can be an alternative in treating neurodegenerative diseases. This study aimed to characterize NPs from neurospheres obtained after seeding ADMSCs on a natural polyisoprene-based membrane. The ADMSCs were isolated from adipose tissue by enzymatic dissociation, were subjected to trilineage differentiation, and were characterized by flow cytometry for specific ADMSC surface markers. For neuronal differentiation, the cells were seeded on polystyrene flasks coated with the membrane and were characterized by immunocytochemistry and RT-PCR. The results demonstrated that the isolated cells showed characteristics of ADMSCs. At 15 to 25 days, ADMSCs seeded on the natural membrane developed neurospheres. Then, after dissociation, the cells demonstrated characteristic neuronal markers expressed on NPs: nestin, ß-III tubulin, GFAP, NeuN, and the YAP1/AMOT in the cytoplasm. In conclusion, it was demonstrated that this membrane differentiates the ADMSCs to NPs without any induction factors, and suggests that their differentiation mechanisms are related to mechanotransduction regulated by the YAP and AMOT proteins.

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Relevant biological processes for tissue development with stem cells and their mechanistic modeling: A review

Cited by 6 publications

References 175 publications

Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins

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