Relevance of π–π and dipole–dipole interactions for retention on cyano and phenyl columns in reversed-phase liquid chromatography

Croes, Kathleen; Steffens, A.; Marchand, Daniel H.; Snyder, Lloyd R.

doi:10.1016/j.chroma.2005.08.090

Cited by 132 publications

(63 citation statements)

References 8 publications

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“…An increase in ACN proportion in the mobile phase was found to decrease retention of FSVs on the PFP fused core column, probably due to competitive π-π interaction of ACN with the solutes and stationary phase (28). The optimal ACN content in the mobile phase was found to be 10 %.…”

Section: Monolithic Versus Fused Core Columnsmentioning

confidence: 92%

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

et al. 2017

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HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (R s > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.Keywords: monolithic silica column, fused core column, HPLC, fat-soluble vitamins, analytical performance There is an increasing demand for fast chromatographic methods suitable for routine analyses with low analysis cost. So far, HPLC is the main instrumental technique used widely for the separation and quantification of drugs. Accordingly, there is an enormous need for highly efficient, selective LC columns to provide faster and more precise analyses, especially for high throughput samples in research laboratories and pharmaceutical industry. Three approaches are currently in competition to achieve fast chromatographic analysis UPLC (ultra performance liquid chromatography), fused core columns and monolithic columns, attached to HPLC (1, 2).UPLC relies on the use of short columns with sub-2-μm particles. The UPLC instrument is designed to resist pressure up to 1.5 × 10 8 Pa, which exceeds the pressure limits for SAID EL KURDI 1

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Section: Monolithic Versus Fused Core Columnsmentioning

confidence: 92%

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

et al. 2017

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“…[21] for a broad review of the technique used and the results obtained, and to ref. [24] for a discussion of additional specific interactions for specific packing types. A very brief overview can also be found in ref.…”

Section: General Characterization Methods For Stationary Phasesmentioning

confidence: 99%

Stationary phase characterization and method development

Neue

2007

J of Separation Science

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The properties of stationary phases and their characterization methods are reviewed. New and significant developments have occurred in the last few years, and new methods for stationary phase characterization have become available. The characterization methods are discussed, and the differences between the different methods are pointed out. In addition, method development approaches are reviewed, with special emphasis on recent developments that employ multiple parameters in parallel. Also, the renewed interest of temperature as a tool in method development is surveyed.

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“…The chromatographic parameters for the columns are shown in Table 2. The successful separation of the sulfonamides is not only attributed to the best physical properties of the columns (i.e., shorter diffusion path and partial porosity) but to the different structures in the stationary phases that enabled differential interactions with the analytes (Croes et al 2005;Zhang et al 2014).…”

Section: Analytical Letters 681mentioning

confidence: 99%

“…The aliphatic chain presented hydrophobic characteristics and dipole-dipole interactions while the phenyl group may interact through a π-π stacking mechanism (Croes et al 2005).…”

Section: Preparation and Characterization Of The Solid Phasementioning

confidence: 99%

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

et al. 2015

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The synthesis of amide (butyl, cyclohexyl, and phenyl) modified silica and the use as stationary phases in sequential injection chromatography are described. The system was tested on the isocratic separation of seven sulfonamides (sulfachloropyridazine, sulfadimethoxine, sulfamethazine, sulfamethoxazole, sulfamethoxypyridazine, sulfaquinoxaline, and sulfathiazole) using each stationary phase with mobile phases composed of acetonitrile/water at a flow rate of 0.45 mL min −1. A mixed mode retention mechanism of sulfonamides in the stationary phases was obtained, including dipole-dipole, π-π, and hydrogen bonding interactions. The most appropriate phase for the separation of sulfonamides was phenylamide. The chromatographic behavior was confirmed using density functional theory of the interaction between sulfamethoxazole and the stationary phases. ARTICLE HISTORY

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Relevance of π–π and dipole–dipole interactions for retention on cyano and phenyl columns in reversed-phase liquid chromatography

Cited by 132 publications

References 8 publications

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

Stationary phase characterization and method development

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

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