Relevance of serologic studies in inflammatory bowel disease

Vernier, Gwenola; Sendid, Boualem; Poulain, Daniel; Colombel, Jean–Frédéric

doi:10.1007/s11894-004-0070-x

Cited by 41 publications

(31 citation statements)

References 36 publications

(29 reference statements)

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“…Therefore, the expression of MadCAM-1 in the extra-intestinal organs may be significant characteristics of SAMP1/ Yit mice. TNF- induces the expression of MAdCAM-1, and is detected with the co-expression of MAdCAM-1 in the intestinal pre-histopathogenetical regions of SAMP1/Yit mice and human Crohn's disease [7,31,32]. In the extraintestinal organs of SAMP1/Yit mice, TNF- was scattered in a reticular pattern, similar to the non-affected regions of AKR/J mice.…”

Section: Discussionmentioning

confidence: 90%

“…Therefore, involvement of TNF- with induction of MadCAM-1 is unclear in the extraintestinal lesions of SAMP1/Yit mice. In the intestinal lesion of human Crohn's disease and SAMP1/Yit mice, Th1 type immunoreaction is believed to underlie the pathogenesis of intestinal inflammation, since Th1 type cytokines, such as TNF-, IL-12 and LT-, were detected in the intestinal lesions [31,32]. Conversely, in the extra-intestinal lesions of the SAMP1/Yit mice, CD4-positive T lymphocytes expressed IL-4, one of Th2 type cytokines.…”

Section: Discussionmentioning

confidence: 99%

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Pathomechanism of Cellular Infiltration in the Perivascular Region of Several Organs in SAMP1/Yit Mouse

Chosa

Soeta

Ichihara

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. We investigated the histological changes of extra-intestinal organs, such as the liver, kidney, lung and pancreas in SAMP1/ Yit mice, a human Crohn's disease model, using immunohistochemical techniques. The perivascular cellular infiltration was detected around the small vessels after 30 weeks. These infiltrating cells consisted of many CD4-positive T-lymphocytes, and small numbers of CD8-positive T-lymphocytes and IgG-positive B-lymphocytes. MAdCAM-1 and VCAM-1 were detected in vascular endothelial cells in non-affected regions of 13 and 20 week-old, as well as in the affected regions showing perivascular cellular infiltration after 30 weeks. In addition, integrin 47 was detected on these infiltrating cells in the perivascular regions after 30 week-old. LT- and IL-12, cytokines of the Th-1-type immune response, were not observed in these affected regions. However, IL-4, one of the cytokines of the Th-2-type immune response, was detected on the perivascular infiltrating cells after 30 week-old. These results revealed that the changes in extra-intestinal organs were mainly caused by infiltration of CD4-positive T-lymphocytes into the perivascular regions in SAMP1/Yit mice. These cellular infiltrations were thought to be initiated by adhesion of CD4-positive T-lymphocytes to the endothelial cells mediated by MAdCAM-1 and integrin 7. Immunohistochemistry for Th related cytokines indicated that the perivascular cellular infiltration was developed by the Th-2-type immune response in the extra-intestinal organs of SAMP1/Yit mouse.KEY WORDS: cell infiltration, pathomechanism, SAMP1/Yit mouse.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Pathomechanism of Cellular Infiltration in the Perivascular Region of Several Organs in SAMP1/Yit Mouse

Chosa

Soeta

Ichihara

et al. 2009

The Journal of Veterinary Medical Science

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“…The results are in line with previous reports, which indicate that serum bacterial antigen-specific IgG (including flagellin-specific IgG) can be a reference parameter in the diagnosis of IBD. 27 It is possible that the colon is a major site of the flagellin-specific IgG production since its highest density of microbes in the body. The produced IgG may be partially absorbed into the blood stream and detected in the serum as reported, 27,28 and partially bound to immune cells, such as mast cells, in local tissue as shown by the present study.…”

Section: Discussionmentioning

confidence: 99%

Fc Gamma Receptor Signaling in Mast Cells Links Microbial Stimulation to Mucosal Immune Inflammation in the Intestine

Chen

Feng

Zheng

et al. 2008

The American Journal of Pathology

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Microbes and microbial products are closely associated with the pathogenesis of inflammatory bowel disease (IBD); however, the mechanisms behind this connection remain unclear. It has been previously reported that flagellin-specific antibodies are increased in IBD patient sera. As mastocytosis is one of the pathological features of IBD, we hypothesized that flagellin-specific immune responses might activate mast cells that then contribute to the initiation and maintenance of intestinal inflammation. Thirtytwo colonic biopsy samples were collected from IBD patients. A flagellin/flagellin-specific IgG/Fc gamma receptor I complex was identified on biopsied mast cells using both immunohistochemistry and co-immunoprecipitation experiments; this complex was shown to co-localize on the surfaces of mast cells in the colonic mucosa of patients with IBD. In addition, an ex vivo study showed flagellin-IgG was able to bind to human mast cells. These cells were found to be sensitized to flagellin-specific IgG; re-exposure to

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“…Colonization of mice with C. albicans has been shown to result in dietary Ag leak from the stomach [148]. CD is associated with a microbiotic dysbiosis and the development of antibodies against members of the microbiota [161]. This includes anti-S. cerevisiae antibodies, which have been shown to be reactive to an in vivo expressed epitope on Candida species, as well as baker's yeast [149].…”

Section: Microorganisms and The Immune System Exhibit Crosstalk Betwementioning

confidence: 99%

Richness and diversity of mammalian fungal communities shape innate and adaptive immunity in health and disease

2014

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Human holobiomes are networks of mutualistic interactions between human cells and complex communities of bacteria and fungi that colonize the human body. The immune system must tolerate colonization with commensal bacteria and fungi but defend against invasion by either organism. Molecular ecological surveys of the human prokaryotic microbiota performed to date have revealed a remarkable degree of bacterial diversity and functionality. However, there is a dearth of information regarding the eukaryotic composition of the microbiota. In this review, we describe the ecology and the human niches of our fungal "fellow travelers" in both health and disease, discriminating between passengers, colonizers, and pathogens based on the interaction of these fungi with the human immune system. We conclude by highlighting the need to reconsider the etiology of many fungal and immune-related diseases in the context of the crosstalk between the human system and its resident microbial communities.Keywords: Dysbiosis r Fungal metagenomics r Host-microbe interaction r Mycobiota IntroductionHumans live in close association with a complex community of bacteria, viruses, fungi, and archaea [1][2][3], which inhabit their bodies. Many groups have surveyed these microbial populations using the so-called "next generation" or "deep" sequencing approaches, revealing that the human microbiota differs radically at various body sites and among individuals [2][3][4]. The differences in the human microbiota are influenced by the availability of nutrients, environmental exposure to microorganisms, and other sitespecific features, such as the immunological makeup of a given location. The origin of differences in the microbiota between individuals potentially reflects different patterns of colonization early in life (reviewed in [5]), different dietary regimens [6,7], and different environmental exposures, such as antibiotic use [8,9]. Recently, the application of deep sequencing approaches have genCorrespondence: Dr. Duccio Cavalieri e-mail: duccio.cavalieri@fmach.it erated novel hypotheses about the microbial determinants underlying human disorders of immune origin (reviewed in [10]). Several metabolites of the interaction between diet and host microbiota, such as short-chain fatty acids, have been shown to play a fundamental role in shaping immune responses (reviewed in [11]). The application of microbial ecology concepts is ultimately leading to the conclusion that health and disease can be understood only through an understanding of the ways in which the symbiotic interactions between microbes and human organs harmonically integrate in the context of the hologenome [12].Human microbial diversity is not limited to bacteria; microorganisms such as fungi also play major roles in the stability of microbial communities in human health and disease (reviewed in [13]). Yeasts were detected in human stool samples as far back as 1917, and by the mid-20th century the presence of yeasts in the human intestine was proposed to have a saprotrophic role [14]....

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Relevance of serologic studies in inflammatory bowel disease

Cited by 41 publications

References 36 publications

Pathomechanism of Cellular Infiltration in the Perivascular Region of Several Organs in SAMP1/Yit Mouse

Pathomechanism of Cellular Infiltration in the Perivascular Region of Several Organs in SAMP1/Yit Mouse

Fc Gamma Receptor Signaling in Mast Cells Links Microbial Stimulation to Mucosal Immune Inflammation in the Intestine

Richness and diversity of mammalian fungal communities shape innate and adaptive immunity in health and disease

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