Relevance of nutrient media composition for hydrogen production in Chlamydomonas

González-Ballester, David; Jurado-Oller, Jose Luis; Fernández, Emilio

doi:10.1007/s11120-015-0152-7

Cited by 35 publications

(15 citation statements)

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“…S deprivation is a strategy widely used to enhance photobiological-H 2 production in Chlamydomonas [ 35 , 36 ], which can lead to the highest H 2 yields ( Table 1 ). However, this strategy has several drawbacks, including growth inhibition and the loss of the cell viability (caused by the S prolonged deficiency), which reduce the potential for H 2 generation.…”

Section: Characteristics Of the Algae–bacteria Association For H mentioning

confidence: 99%

“…Different culturing approaches have also been developed to alleviate the identified bottlenecks. Among these approaches are the modulation of the light intensity [ 14 , 27 , 28 , 29 , 30 , 31 ], the optimization of the photosynthetic electrons flow towards the HYDAs [ 29 , 32 , 33 , 34 ], the implementation of nutrient stresses, especially sulfur (S) deprivation, influencing H 2 production [ 35 , 36 , 37 , 38 , 39 ], the addition of O 2 scavengers into the culture media [ 33 , 40 , 41 ], or cell immobilization [ 42 , 43 , 44 ]. Moreover, in recent years, the co-cultivation of alga and bacteria has arisen as an alternative strategy to increase algal H 2 production.…”

Section: Introductionmentioning

confidence: 99%

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Algae-Bacteria Consortia as a Strategy to Enhance H2 Production

Fakhimi

González-Ballester

Fernández

et al. 2020

Cells

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Biological hydrogen production by microalgae is a potential sustainable, renewable and clean source of energy. However, many barriers limiting photohydrogen production in these microorganisms remain unsolved. In order to explore this potential and make biohydrogen industrially affordable, the unicellular microalga Chlamydomonas reinhardtii is used as a model system to solve barriers and identify new approaches that can improve hydrogen production. Recently, Chlamydomonas–bacteria consortia have opened a new window to improve biohydrogen production. In this study, we review the different consortia that have been successfully employed and analyze the factors that could be behind the improved H2 production.

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Section: Characteristics Of the Algae–bacteria Association For H mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Algae-Bacteria Consortia as a Strategy to Enhance H2 Production

Fakhimi

González-Ballester

Fernández

et al. 2020

Cells

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“…PFR activity is coupled to FDX1, which accepts the electrons from the catalyzed reaction and donates them to HYDA1. It has been shown that PFR is also able to use, in addition to pyruvate, oxaloacetate as a substrate (reviewed in [1]).…”

Section: Introductionmentioning

confidence: 99%

“…Mixotrophic and autotrophic growth conditions refer to the presence or absence of acetate, respectively. Importantly, most studies about H 2 production in Chlamydomonas have been done using Tris-Acetate-Phosphate (TAP) medium, and it is well known that acetate strongly enhances H 2 production in Chlamydomonas (reviewed in [1]).…”

Section: Introductionmentioning

confidence: 99%

H2 production pathways in nutrient-replete mixotrophic Chlamydomonas cultures under low light. Response to the commentary article “On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions,” by Alberto Scoma and Szilvia Z. Tóth

González-Ballester

Jurado-Oller

Galván

et al. 2017

Biotechnol Biofuels

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BackgroundA recent Commentary article entitled “On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions” by Dr. Scoma and Dr. Tóth, Biotechnology for Biofuels (2017), opened a very interesting debate about the H2 production photosynthetic-linked pathways occurring in Chlamydomonas cultures grown in acetate-containing media and incubated under hypoxia/anoxia conditions. This Commentary article mainly focused on the results of our previous article “Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures,” by Jurado-Oller et al., Biotechnology for Biofuels (7, 2015; 8:149).Main bodyHere, we review some previous knowledge about the H2 production pathways linked to photosynthesis in Chlamydomonas, especially focusing on the role of the PSII-dependent and -independent pathways in acetate-containing nutrient-replete cultures. The potential contributions of these pathways to H2 production under anoxia/hypoxia are discussed.ConclusionDespite the fact that the PSII inhibitor DCMU is broadly used to discern between the two different photosynthetic pathways operating under H2 production conditions, its use may lead to distinctive conclusions depending on the growth conditions. The different potential sources of reductive power needed for the PSII-independent H2 production in mixotrophic nutrient-replete cultures are a matter of debate and conclusive evidences are still missing.

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“…In the early 1940s, Hans Gaffron and his group observed that Chlamydomonas reinhardtii had the ability to produce hydrogen under anaerobic conditions (Gaffron and Rubin 1942 ). The production of hydrogen from water and sunlight using microalgae has been the subject of applied research since the early 1970s (Benemann 1996 ), but since the discovery of hydrogen production by sulfur deprivation (Ghirardi et al 2000 ; Melis et al 2000 ), which allowed a significant amount of hydrogen to be produced from light by biophotolysis, this research has advanced substantially (Burgess et al 2011 ; Eroglu and Melis 2011 ; Dubini and Ghirardi 2015 ; Gonzalez-Ballester et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Design and construction of a photobioreactor for hydrogen production, including status in the field

et al. 2016

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Several species of microalgae and phototrophic bacteria are able to produce hydrogen under certain conditions. A range of different photobioreactor systems have been used by different research groups for lab-scale hydrogen production experiments, and some few attempts have been made to upscale the hydrogen production process. Even though a photobioreactor system for hydrogen production does require special construction properties (e.g., hydrogen tight, mixing by other means than bubbling with air), only very few attempts have been made to design photobioreactors specifically for the purpose of hydrogen production. We have constructed a flat panel photobioreactor system that can be used in two modes: either for the cultivation of phototrophic microorganisms (upright and bubbling) or for the production of hydrogen or other anaerobic products (mixing by “rocking motion”). Special emphasis has been taken to avoid any hydrogen leakages, both by means of constructional and material choices. The flat plate photobioreactor system is controlled by a custom-built control system that can log and control temperature, pH, and optical density and additionally log the amount of produced gas and dissolved oxygen concentration. This paper summarizes the status in the field of photobioreactors for hydrogen production and describes in detail the design and construction of a purpose-built flat panel photobioreactor system, optimized for hydrogen production in terms of structural functionality, durability, performance, and selection of materials. The motivations for the choices made during the design process and advantages/disadvantages of previous designs are discussed.

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Relevance of nutrient media composition for hydrogen production in Chlamydomonas

Cited by 35 publications

References 122 publications

Algae-Bacteria Consortia as a Strategy to Enhance H2 Production

Algae-Bacteria Consortia as a Strategy to Enhance H2 Production

H2 production pathways in nutrient-replete mixotrophic Chlamydomonas cultures under low light. Response to the commentary article “On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions,” by Alberto Scoma and Szilvia Z. Tóth

Design and construction of a photobioreactor for hydrogen production, including status in the field

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