Relevance of Interleukins 6 and 8 Single Nucleotide Polymorphisms in Prostate Cancer: A Multicenter Study

Abstract

The diverse roles of cytokines as IL-6 and IL-8 have been studied in terms of their SNPs in many diseases but their role in prostate cancer (PCa) is still uncertain. Aim. To determine the relevance of IL-6 rs1800795 SNP and/or IL-8 rs2227306 SNP with prostate cancer’s risk. Subjects and Methods. 40 PCa patients, 40 benign prostate hyperplasia (BPH) patients, and 40-age-matched-control group were enrolled in the study. Genotyping of IL-6 rs1800795 (G/C) SNP and IL-8 rs2227306 (C/T) SNP was determined using real-time PCR. Results. High frequency of IL-6 rs1800795GG and IL-8 rs2227306CC genotypes was noticed among PCa patients with associated OR 10.091 and 8.143, respectively. Comparisons based on allele frequencies revealed that IL-6G and IL-8C alleles are more frequent among PCa patients than other groups. Presence of IL-6 rs1800795G and IL-8 rs2227306C alleles in the same patient increase PCa risk by 16.7 times. Statistical correlations between PSA ratio and both of IL-6 and IL-8 SNP did not show any significant relation among PCa patients. Conclusion. IL-6 rs1800795G and IL-8 rs2227306C alleles could be considered risk factors for PCa development, particularly if presented together. However, no relation was found between both cytokines SNP and severity of prostate cancer.

Influence of IL-6 rs1800795 and IL-8 rs2227306 polymorphisms on COVID-19 outcome

“…Polymorphisms in IL-6 and/or IL-8 genes may account for the variability of their expression and disparities in the pathogenesis of various infectious diseases [8,[13][14][15]. Recently, IL-6 rs1800795G and IL-8rs 2227306C alleles were reported to be risk factors for prostate cancer [8].…”

“…Genomic DNA was extracted from the blood samples using QIAamp DNA Blood Mini Kit (Applied Biosystems, ThermoFisher Scientific, Foster City, USA). IL-6 rs1800795 (G/C) and IL-8 rs2227306 (C/T) SNPs genotyping was performed using TaqMan duallabeled probes, as described by Ghazy et al (2021) [8]. Genotypes were determined by the increased fluorescence signal when the probes hybridized to the complementary sequence in the tested samples and were cleaved from the dye.…”

“…IL-8 has a role in inflammation, recruitment of immune cells, neutrophil activation, and degranulation [8]. In SARS-CoV-2 infection, it was reported that the prothrombotic, degranulated neutrophil phenotype in severe COVID-19 is associated with increased IL-8 release and expression on neutrophils recruited to the pulmonary tissue which, in turn, activates IL-8 production from peripheral neutrophils as sustained loops [6].…”

“…Genetic mutations such as single nucleotide polymorphisms (SNP) could influence the molecular mechanisms and pathogenesis of any disease with variable outcomes in different populations [8]. Recently, we have studied the role of IL-6 and IL-8 SNPs in prostate cancer and found a significant association between IL-6 rs1800795 G and IL-8 rs2227306 C alleles and cancer development, particularly when they coexist [8].…”

Role of IL-6 and IL-8 polymorphisms in Type I Diabetes mellitus

“…Genotyping of IL-6 rs0795 and IL-8 rs7306 SNP Genomic DNA was extracted from the blood samples on EDTA using spin columns of QIAamp DNA Blood Mini Kit (Applied Bio Systems-Life Technologies); according to the manufacturer's instructions. The concentration and purity of DNA were assessed by nanodrop before real-time PCR (Ghazy and Alenzi, 2021).…”