Relevance of Arginines in the Mode of Binding of H1 Histones to DNA

Piscopo, Marina; Conte, Mariachiara; Paola, Flaviano Di; Conforti, Salvatore; Rana, Gina; Petrocellis, Luciano De; Fucci, Laura; Geraci, Giuseppe

doi:10.1089/dna.2009.0993

Cited by 23 publications

(27 citation statements)

References 44 publications

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“…This suggested that hyposalinity exposure did not affect DNA binding mode of PL proteins, different from what we have seen while exposing mussels to copper chloride . In that case, we observed an increase in DNA binding affinity and also a change of DNA binding mode from "all or nothing" to "intermediate mode", the typical DNA binding mode of somatic H1 histone (Piscopo et al, 2010).…”

Section: Discussionmentioning

confidence: 72%

“…galloprovincialis spermatozoa and on protamine‐like proteins (PL‐ proteins). These proteins are related to histone H1 and are rich in arginine and lysine (30–50 mol%; Fioretti et al, ; Piscopo et al, ). They represent the 76% of the overall nuclear protein in M .…”

Section: Introductionmentioning

confidence: 99%

“…Given that the effects of hyposalinity on mussels reproduction need to be better understood and documented, in this study we studied the molecular effects that hyposalinity can have on M. galloprovincialis spermatozoa and on protamine-like proteins (PL-proteins). These proteins are related to histone H1 and are rich in arginine and lysine (30-50 mol%;Fioretti et al, 2012;Piscopo et al, 2010). They represent the 76% of the overall nuclear protein in M. galloprovincialis with PL-III (11.3 kDa) present in the highest amount (50%) and PL-II (14 kDa), and PL-IV (6.5 kDa), which represent the 20% and 6%, respectively (Vassalli et al, 2015).…”

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confidence: 99%

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Molecular effects on spermatozoa of Mytilus galloprovincialisexposed to hyposaline conditions

Lettieri

Maione

Ranauda

et al. 2019

Molecular Reproduction Devel

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Salinity represents a critical environmental and an ecological factor in the reproduction of marine species. As global climate changes and anthropogenic factors affect salinity, in this study, we have analyzed the responses of Mytilus galloprovincialis spermatozoa to hyposaline stress. We exposed mussels, in laboratory tanks, for 24 hr at 18°C to control (35.9 psu) and three hyposaline (17.1, 22.6, and 26.2 psu) conditions, and evaluated the expression of sperm hsp70 and protamine‐like proteins genes. Further we analyzed the electrophoretic pattern, the DNA binding and the release from sperm nuclei of protamine‐like proteins. For all experimental approaches used, the results obtained at 17.1 psu condition were very similar to those obtained in the control condition, while alterations were always recorded at 22.6 and 26.2 psu conditions. Particularly, at 22.6 and 26.2 psu, was observed: 42.5‐ and 17.1‐fold increase in hsp70 expression, respectively, and hypoexpression of PL‐II/PLIV protamine‐like proteins genes. Further, electrophoretic mobility shift assays and salt‐induced release of nuclear proteins from sperm nuclei, revealed alterations in the PL proteins/DNA binding, in these two hyposaline conditions. The similarity between the results obtained in control and in the more severe hyposaline condition (17.1 psu) could indicate a phenomenon of fertility preservation strategy due to gamete plasticity.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular effects on spermatozoa of Mytilus galloprovincialisexposed to hyposaline conditions

Lettieri

Maione

Ranauda

et al. 2019

Molecular Reproduction Devel

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“…However, nothing has been presented to date on the bactericidal activity of protamine-like proteins (PL-proteins), which are one of the three types of Sperm Nuclear Basic Proteins (SNBPs) and represent a structurally and functionally intermediate group of proteins between the histone (H) and protamine (P) type (Ausió, 1999). These proteins are related to histone H1 and are arginine-and lysine-rich Piscopo et al, 2010). They were first described in bivalve mollusks but later have been also found in echinoderms, tunicates, and vertebrates (Ausió, 1995;Eirin-Lo´pez et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Protamine-like proteins have bactericidal activity. The first evidence in Mytilus galloprovincialis

et al. 2018

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The major acid-soluble protein components of the mussel Mytilus galloprovincialis sperm chromatin consist of the protamine-like proteins PL-II, PL-III and PL-IV, an intermediate group of sperm nuclear basic proteins between histones and protamines. The aim of this study was to investigate the bactericidal activity of these proteins since, to date, there are reports on bactericidal activity of protamines and histones, but not on protamine-like proteins. We tested the bactericidal activity of these proteins against Gram-positive bacteria: Enterococcus faecalis and two different strains of Staphylococcus aureus, as well as Gram-negative bacteria: Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhmurium, Enterobacter aerogenes, Enterobacter cloacae, and Escherichia coli. Clinical isolates of the same bacterial species were also used to compare their sensitivity to these proteins. The results show that Mytilus galloprovincialis protamine-like proteins exhibited bactericidal activity against all bacterial strains tested with different minimum bactericidal concentration values, ranging from 15.7 to 250 µg/mL. Furthermore, these proteins were active against some bacterial strains tested that are resistant to conventional antibiotics. These proteins showed very low toxicity as judged by red blood cell lysis and viability MTT assays and seem to act both at the membrane level and within the bacterial cell. We also tested the bactericidal activity of the product obtained from an in vitro model of gastrointestinal digestion of protamine-like proteins on a Gram-positive and a Gram-negative strain, and obtained the same results with respect to undigested protamine-like proteins on the Gram-positive bacterium. These results provide the first evidence of bactericidal activity of protamine-like-proteins.

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“…Whereas the on-dyad axis location creates more compacted (transcriptionally inert) chromatin conformation, the off-dyad axis position forms more loosened (transcriptionally capable) chromatin structure (Zhou et al, 2015). Moreover, the mode of histone H1 binding to the DNA is determined by its C-terminal (Th'ng et al, 2005) and globular (Piscopo et al, 2010) domain and may be different in distinct cell types. Due to the presence of the arginine residues, the sperm H1-like histones are able to form aggregates and highly condensed DNA in comparison to the somatic H1 histones deprived of arginine and are thus unable to selfassociate and create highly packaged DNA (Salvati et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Evidence on the stability of histone H1.a polymorphic variants during selection in quail

Kowalski

Knaga

2017

Arch. Anim. Breed.

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Abstract. The goal of this work was to check whether selection for quantitative traits may cause a change in the histone H1 allelic complement and whether it can therefore be considered a modulator of histone H1-dependent chromatin functioning. For this purpose, a fluctuation of histone H1.a polymorphic variants was analyzed among a non-selected (control) quail line and the line selected for a high cholesterol content in the egg yolk. The histone H1.a was found to be polymorphic due to its differential migration rate in the AU-PAGE (acetic acidurea polyacrylamide gel electrophoresis). Based on this, two H1.a isoforms (H1.a1 and H1.a2) that form three phenotypes (a1, a2 and a1a2) were distinguished in the quail lines tested. A comparably expressed (p > 0.05) and low relative variable (coefficient of variation, CV < 0.25) histone H1.a phenotypes were in agreement with Hardy-Weinberg equilibrium (HWE) in both the non-selected (χ 2 = 1.29, p = 0.25) and selected (χ 2 = 1.9, p = 0.16) quail line. The similarity among quail lines was assessed based on the equal distribution of histone H1.a phenotypes (χ 2 = 1.63, p = 0.44) and alleles (χ 2 = 0.018, p = 0.89) frequency in both quail lines tested. This indicates that selection does not affect the histone H1.a polymorphic variants. The stability of histone H1.a during selection might suggest that likely chromatin processes coupled to the selected trait are not linked to the activity of histone H1.a.

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Relevance of Arginines in the Mode of Binding of H1 Histones to DNA

Cited by 23 publications

References 44 publications

Molecular effects on spermatozoa of Mytilus galloprovincialisexposed to hyposaline conditions

Molecular effects on spermatozoa of Mytilus galloprovincialisexposed to hyposaline conditions

Protamine-like proteins have bactericidal activity. The first evidence in Mytilus galloprovincialis

Evidence on the stability of histone H1.a polymorphic variants during selection in quail

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