Release test of alliin/alliinase double-layer tablet by HPLC—Allicin determination

Abstract

A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100 r/min using 100 mL phosphate buffer (pH 6.8) as release medium. The release amount was determined by HPLC with a C18 column (250 mm×4.6 mm, 5 μm) using the mobile phase consisting of methanol −0.4% carboxylic acid (65:35) at a flow rate of 1 mL/min and UV detection at 242 nm. The current method demonstrates good linearity over the range 4.052–405.2 μg/mL (r2=0.9999) with an average recovery of 105.5%(RSD=1.25%). The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for within- and between-batches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets.

Purification and characterisation of alliinase produced by Cupriavidus necator and its application for generation of cytotoxic agent: Allicin

“…Extreme temperature and pH might be having an effect on the structure of enzyme, rendering it inactive. This temperature and pH sensitivity of alliinase can be encountered during oral administration, by a method reported by Lianga et al (2013) wherein an enteric coated double-layer tablet of alliin/alliinase was used, for optimum activity of alliinase on alliin under gastrointestinal condition.…”

Allicin affects the pharmacokinetics of sulfadiazine and florfenicol by downregulating the expression of jejunum P-gp and BCRP in broilers

“…Alliinase and alliin were obtained from Ailexin Drug Company (Xinjiang, China). It has been reported that 1 g of alliin reacts with alliinase to form 0.458 g of allicin ( Liang et al, 2013 ). For the animal studies, allicin was prepared by incubating appropriate quantity of alliin with alliinase in water under magnetic stirring for 30 min at room temperature.…”

Characterization and antimicrobial potential of bioactive components of sonicated extract from garlic ( Allium sativum ) against foodborne pathogens

“…Allicin was quantified as per protocol of Liang et al (). Prepared samples were run on high‐performance liquid chromatographic system (HPLC) system (Shimadzu, Japan) operated with LC pump‐10 AT and detector SPDIO‐AV.…”