“…The use of enzymes that lyse cell walls is a promising alternative to chemical disruption as a means of recovering intracellular microbial products (10,13,18,20,28,36). The ,-1,4-linkages between the N-acetylmuramic acid and N-acetylglucosamine residues of the peptidoglycan layer of bacterial gram-positive cell walls are hydrolyzed by egg white lysozyme, and this enzyme is widely used for lysis of gram-positive bacteria (26).…”