Release of Ultraviolet-Absorbing Material From Escherichia Coli at Subzero Temperatures

Lindeberg, Gunnar; Lode, Aaslaug

doi:10.1139/m63-067

Cited by 21 publications

(6 citation statements)

References 11 publications

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“…It is clearly demonstrated in the present study that inhibilioii of endogenous respiration was paralleled bj' leaking of VY absorbing substances from the cells. The absorbance characteristics of these substances (Figtu'e 6) are very similar to those obtained for instance for materials released from Escherichia coli upon freezing and thawing (Lindeberg and Lode 1963), and from Merulius Utcrymans when grown at supraoplimal temperatures (Langvad and Goksoyr 1967). Consequently, vai'ious organisms seem to respond to quite different treatments with the same type of leaking.…”

Section: Discussionsupporting

confidence: 71%

Effect of Fatty Acids and Methyl Octanoate on Resting Mycelium of Boletus variegatus

Pedersen

1970

Physiologia Plantarum

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The endogenous respiration of resting, submerged grown Boletus variegatus mycelium has been determined. In young cultures the intensity of the endogenous oxygen uptake was subject to great variations during the first few hours of starvation. However, by using six to eight days old mycelium the Qo2 values could be kept at a relatively low and constant level for at least nine hours. Inhibition of the endogenous respiration was found after addition of n‐saturated C‐2 to C‐12 fatty acids (2 × 10‐3M, pH 4.85). The inhibitory effect of the compound was dependent on the length of the carbon chain. Maximum effects were obtained for acids with eight to twelve carbon atoms per molecule. The inhibition was also dependent on the amount of undissociated acid present. By raising the pH so that the fatty acid dissociated the established inhibition was partly reversed. The effect of the neutral compound methyl octanoate was in essence identical to that obtained with octanoic acid. After fatty acid addition a close correspondence was found between the degree of inhibition of the oxygen uptake and the amount of UV absorbing substances leaking out from the cells. This extracellular material had an absorption maximum at 260 nm and a minimum around 240 nm. The leaking was ascribed to interaction between fatty acids or methyl octanoate and lipophilic substances of the cytoplasmic membrane. It is suggested that the inhibitory action on the endogenous respiration is due to similar effects on intracellular membrane systems.

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Section: Discussionsupporting

confidence: 71%

Effect of Fatty Acids and Methyl Octanoate on Resting Mycelium of Boletus variegatus

Pedersen

1970

Physiologia Plantarum

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“…Absence of death and injury among cells frozen and stored at -198°C and the obvious death and injury among cells that were frozen at -198°C and stored at -18°C suggested that lethal and sublethal effects occurred primarily during frozen storage. This may have been caused by growth of ice crystals and concentration of solutes rather than cold shock which has been suggested as the chief factor in killing bacterial cells during freezing (Hilliard and Davis, 1918;Lindeberg and Lode, 1963). Moss and Speck (1963) Seven freeze-thaw cycles of L. monocytogenes in PB stored at -198°C resulted in 18.7% death and 0% injury (Fig.…”

Section: Resultsmentioning

confidence: 99%

Fate of Listeria monocytogenes During Freezing and Frozen Storage

El-Kest

Yousef

Marth

1991

Journal of Food Science

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A cell suspension of Lkteria monocytogenes was frozen 30 min at -WC, or 10 min in liquid nitrogen (LN) at -198°C. Solidification required 15 min at -18°C and ca. 1 min at -198°C. Freezing and storage 1 month in phosphate buffer (PB) at -18°C caused 87% death and 79% injury. These were 54 and 4.5%, respectively, for cells in Tryptose Broth (TB) at -18°C. Freezing and storage 1 month in LN caused no death or injury of cells suspended in PB, whereas some injury and death occurred in TB. Freezing at -198°C followed by storage 1 month at -18°C resulted in 60% death and 36% injury in PB, and 61 and 44.2%, in TB. Repeated freezing and thawing caused more death/injury than did a single freeze-thaw cycle.

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“…Damage to membranes has been shown to lead to the leakage of internal cell materials such as potassium ions, P-galactosidase and low molecular UV-absorbing materials (Lindeberg & Lode, 1963;Calcott & MacLeod, 1975). The release of these substances has been correlated negatively with cell viability.…”

Section: Studies Of Microbial Growth and Survival In Frozen Food Systemsmentioning

confidence: 99%

Position paper: Towards predictive microbiology in frozen food systems – a framework for understanding microbial population dynamics in frozen structures and in freeze‐thaw cycles

Archer¹,

Kennedy²,

Wilson³

1995

Int J of Food Sci Tech

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Release of Ultraviolet-Absorbing Material From Escherichia Coli at Subzero Temperatures

Cited by 21 publications

References 11 publications

Effect of Fatty Acids and Methyl Octanoate on Resting Mycelium of Boletus variegatus

Effect of Fatty Acids and Methyl Octanoate on Resting Mycelium of Boletus variegatus

Fate of Listeria monocytogenes During Freezing and Frozen Storage

Position paper: Towards predictive microbiology in frozen food systems – a framework for understanding microbial population dynamics in frozen structures and in freeze‐thaw cycles

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