Release of polysaccharide by sonication of cells (Porphyridium sp.)

Faerman, V.; Mukmenev, Igor; Shreĭber, I. R.

doi:10.1134/s1063771009020171

Cited by 2 publications

(1 citation statement)

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“…The exopolysaccharides of Currently, the soluble or solubilized exopolysaccharides are classically separated from cells by centrifugation or microfiltration [5,13,14]. Bounded EPS can be solubilized by addition of formaldehyde, ethylene diamine tetracetic acid and sodium hydroxide or by sonication, heating, complexation and ion exchange [1,15]. Then, soluble or solubilized exopolysaccharides are traditionally extracted and purified using dialysis against distilled water or precipitation with alcohols (methanol, ethanol and isopropanol) or cationic surfactants [5,16,17].…”

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confidence: 99%

Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities

Balti

Balc’h

Brodu

et al. 2018

Process Biochemistry

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Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74 to 2.26 g.L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80 % (w/w) of polysaccharides were recovered while 49 % and 99 % of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9 % against 0.6 % (w/w) initially.2 Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site. Graphical abstractHighlights ► Simple and rapid micro-alga's exopolysaccharide (EPS) concentration and purification ► High yields of EPS recovery and good permeate fluxes ► Change of rheological behaviour and composition of filtered solutions during filtration ► Time and degree of concentration change following the cross-flow velocity ► Advanced characterization of extracts

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