Release of nifedipine, furosemide, and niclosamide drugs from the biocompatible poly(HEMA) hydrogel structures

Abstract

The primary aim of this article was to improve the solubility and bioavailability of the drugs nifedipine, niclosamide, and furosemide due to their poor solubility and dissolution rate. Therefore, these drugs require improvement in solubility and dissolution rate in formulation development, especially in solid dosage forms such as hydrogels and capsules. Hydrogel structures were synthesized by using a biocompatible, nontoxic, and protective pure 2-hydroxy ethyl methacrylate (HEMA) monomer. These hydrogel structures were characterized by various techniques such as scanning electron microscopy and Fourier-transformed infrared spectroscopy. In the next step, drug molecules were loaded in the poly (HEMA) hydrogels. Drug releases of drug-loaded hydrogel structures were measured at certain time intervals and recorded cumulatively (%). The pH affinity, morphology, structure, drug release, swelling, and cytotoxic effect of the resulting materials were studied in detail. In addition to investigating the biocompatibility and cytotoxicity of the poly (HEMA) hydrogel, we evaluated an in vitro cytotoxicity assay on MCF-7and MIA PaCa-2 and HEK 293 cell lines with the hydrogel structure and confirmed cell viability of over 85%. These results suggest that our HEMA polymeric hydrogel is a material with biological importance and great pharmacological potential as a supporting material in the drug release of nifedipine, furosemide, niclosamide, and similar drug molecules.

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

“…Anticancer activity of the polysaccharide extracts was assayed on HT-29 (colon), HepG2 (liver), and HeLa (cervical) cell lines and HEK-293 (human embryonic kidney) and THLE-2 (liver epithelial) cell lines using the Alamar blue assay . Cells kept at −80 °C were thawed in a 37 °C water bath, centrifuged, and then transferred to the growth medium.…”

“…Anticancer activity of the polysaccharide extracts was assayed on HT-29 (colon), HepG2 (liver), and HeLa (cervical) cell lines and HEK-293 (human embryonic kidney) and THLE-2 (liver epithelial) cell lines using the Alamar blue assay. 75 Cells kept at −80 °C were thawed in a 37 °C water bath, centrifuged, and then transferred to the growth medium. Then, the cells were incubated in DMEM (10% FBS, 1% penicillin–streptomycin, and 0.01% gentamicin) and RPMI (10% FBS, 1% penicillin–streptomycin, and 0.01% gentamicin) mediums at 37 °C in a 5% CO 2 atmosphere.…”

Protein adsorpsiyon çalışmaları için metal şelat grupları içeren pHEMA-GMA kolon dolgu malzemelerinin hazırlanması

“…Karışım -12 0 C'de 24 saat inkübe edildi. 24 saatin sonunda -12 0 C den alınan kriyojeller 2 saat oda sıcaklığında bekletildi [17]. Reaksiyona girmeyen monomerleri ve başlatıcıları uzaklaştırılmak için elde edilen kriyojeller 200 ml su ile 3 kez yıkandı.…”

“…SEM görüntüleri alınacak kriyojel numuneleri öncelikle 5 nm Au ile kaplandı. Kaplanan kriyojeller yaklaşık 1 mikron mesafeden SEM ile görüntülenerek rapor edildi [17].…”

Gingko biloba L.’nın Fitokimyasal Bileşimi, Antioksidan ve Anti-Kanser Aktiviteleri Üzerine Bir Araştırma

“…The anti-cancer activity of the methanol extract against HT-29 (human colon cancer line), HeLa (human cervical cancer line), and HEK-293 (human embryonic kidney cell line) was studied using Alamar Blue experiment (Yılmaz, 2022). Cells kept frozen at -80°C were thawed in a 37°C water bath, centrifuged, then transferred to the growth medium.…”