“…Subsequently, the trimmed specimens were snap frozen in liquid nitrogen and stored at −70 • C for up to 2 months. Researchers have reported that freezing tissue specimens (either snap freezing with liquid nitrogen or in a standard freezer) does not change their diffusion or permeation behaviour when used for such studies (Van der Bijl et al, 1998; Van Eyk and Thompson, 1998; Van Eyk and Van der Bijl, 2004;Consuelo et al, 2005;Giannola et al, 2007).…”
Section: Computation Of the Percentage Drug-loading Capacitymentioning
confidence: 97%
“…After re-hydration, mucosal disks (diameter = 15 mm and surface area = 2.27 cm 2 ) were cut using surgical scissors from the harvested specimen and mounted in the flow through Franz-type diffusion cell apparatus (Membrane transport systems, V3, Permegear, Amie Systems, USA) connected to a heat-circulating water bath/heating system (CPE 100, Labcon, Maraisburg, Gauteng, South Africa). The receiver compartment contained 10 mL simulated plasma, pH 7.4 (Giannola et al, 2007) while the donor compartment contained a 2 mL solution of the drug-loaded P-EAM formulation in simulated saliva, pH 6.8 (Peh and Wong, 1999). Uniform mixing within the receiver compartment was achieved by magnetic stirring.…”
Section: Preparation Of Tissue and Permeation Studiesmentioning
“…Subsequently, the trimmed specimens were snap frozen in liquid nitrogen and stored at −70 • C for up to 2 months. Researchers have reported that freezing tissue specimens (either snap freezing with liquid nitrogen or in a standard freezer) does not change their diffusion or permeation behaviour when used for such studies (Van der Bijl et al, 1998; Van Eyk and Thompson, 1998; Van Eyk and Van der Bijl, 2004;Consuelo et al, 2005;Giannola et al, 2007).…”
Section: Computation Of the Percentage Drug-loading Capacitymentioning
confidence: 97%
“…After re-hydration, mucosal disks (diameter = 15 mm and surface area = 2.27 cm 2 ) were cut using surgical scissors from the harvested specimen and mounted in the flow through Franz-type diffusion cell apparatus (Membrane transport systems, V3, Permegear, Amie Systems, USA) connected to a heat-circulating water bath/heating system (CPE 100, Labcon, Maraisburg, Gauteng, South Africa). The receiver compartment contained 10 mL simulated plasma, pH 7.4 (Giannola et al, 2007) while the donor compartment contained a 2 mL solution of the drug-loaded P-EAM formulation in simulated saliva, pH 6.8 (Peh and Wong, 1999). Uniform mixing within the receiver compartment was achieved by magnetic stirring.…”
Section: Preparation Of Tissue and Permeation Studiesmentioning
“…After 8 h formalin-fixed, paraffin-embedded samples were cut in 4-µm thick sections on a microtome with a disposable blade and conveniently stained with eosin. [18] In vivo drug-release study Six male Newzealand white rabbits (2-2.5 kg) were selected. The dose of Famotidine was adjusted based on the rabbit weight and the best formulations (F5) were placed in the buccal membrane with the adhesive layer.…”
T he present investigation highlights the novel trans-buccoadhesive tablets of Famotidine, an H2-receptor antagonist used as an antiulcerative agent. The buccoadhesive tablets were prepared by direct compression method using bioadhesive polymers like sodium alginate, SCMC, HPMC-K100M, PVP-K30 either alone or in combinations with EC as a backing layer. The prepared formulations were evaluated for their physicochemical characteristics, swelling index, surface pH, ex vivo buccoadhesive strength, in vitro, in vivo drug release and ex vivo permeation studies. The distinguishable differences in the results were shown to be dependent on characteristics and composition of bioadhesive materials used. Stability studies were performed in natural human saliva and accelerated conditions showed no significant difference in physical appearance, drug content, buccoadhesive strength and the P-value statistically significant at <0.05. Ex vivo mucous irritation by histological examination reveals, the administration site of buccal tablet over the buccal mucosa did not cause any irritation, ulceration, inflammation and redness, and it resembles to controlled buccal mucosa. Good correlations were observed between in vitro and in vivo drug release, with a correlation coefficient of 0.996. Drug diffusion from buccal tablets showed apparently zero order kinetics and release mechanism was diffusion controlled after considerable swelling.
“…Continuous research for the improvement of the oral cavity drug delivery has resulted in the development of several dosage forms which can be broadly classified into liquid, semi-solid, solid or spray formulations (Sudhakar et al, 2006). Furthermore, several drugs highly administered by other routes have been assayed through buccal mucosa (Birudaraj et al, 2005;Mashru et al, 2005;Giannola et al, 2007). Within this context, buccal administration could be an alternative, non-invasive delivery route also for doxepin hydrochloride.…”
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