Release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment

Reise, Markus; Wyrwa, Ralf; Kretzer, J. Philippe; Zylinski, Matthias; Völpel, Andrea; Schnabelrauch, Matthias; Berg, Albrecht; Watts, David C.; Sigusch, Bernd W.

doi:10.1016/j.dental.2011.12.006

Cited by 114 publications

(101 citation statements)

References 25 publications

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“…Moreover, we estimated that our materials contained 85 µg MET per cm 2 and released this at a rate of 8 µg per 24 hour period in an aqueous environment. The MIC for MET of this strain has been estimated to be around 60-125 ng/mL while a level of only of 1 µg/ml MET in the sulcus fluid has been reported to be necessary for effective elimination of the most relevant obligate anaerobic periodontal pathogenic bacterial strains [45,46]. In addition, we observed no resistant colonies in this assay, indicating that the level of release of MET in our in vitro antibacterial assay from the nano-layered structure coupled with its lack of toxicity to human cells might represent a potentially effective therapeutic efficient dose.…”

Section: Discussionmentioning

confidence: 99%

“…The outer layer, in contact with bone, was loaded with nanohydroxyapatite to enhance bone regeneration; while the other layer, in contact with epithelial tissue, was functionalised with metronidazole, incorporated in the layer bulk. Interestingly, the direct incorporation of antibiotics to these membranes has given promising results in terms of inhibition of bacteria growth [7,17,18]. However, although the antibacterial effects of these drugs are well known, recent findings have warned of the potentially toxic effects of these highly concentrated antibiotic drugs on dental pulp stem cells and dental pulp fibroblasts [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

et al. 2015

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Article:Gentile, P., Frongia, M.E., Redon, M.C. et al. (4 more authors) (2015) Functionalised nanoscale coatings using Layer-by-Layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces. Acta Biomaterialia.https://doi.org/10.1016/j.actbio.2015.04.009 eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractIn order to achieve high local biological activity and reduce the risk of side effects of antibiotics in the treatment of periodontal and bone infections, a localised and temporally controlled delivery system is desirable. The aim of this research was to develop a functionalised and resorbable surface to contact soft tissues to improve the antibacterial behaviour during the first week after its implantation in the treatment of periodontal and bone infections. Solvent-cast poly(D,L-lactide-coglycolide acid) (PLGA) films were aminolysed and then modified by Layer-by-Layer technique to obtain a nano-layered coating using poly(sodium4-styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) as polyelectrolytes. The water-soluble antibiotic, metronidazole (MET), was incorporated from the ninth layer. Infrared spectroscopy showed that the PSS and PAH absorption bands increased with the layer number. The contact angle values had a regular alternate behaviour from the ninth layer. X-Ray Photoelectron Spectroscopy evidenced two distinct peaks, N 1s and S 2p , indicating PAH and PSS had been introduced. Atomic Force Microscopy showed the presence of polyelectrolytes on the surface with a measured roughness about 10 nm after 20 layers deposition.The drug release was monitored by Ultraviolet-visible spectroscopy showing 80% loaded-drug delivery in 14 days. Finally, the biocompatibility was evaluated in vitro with L929 mouse fibroblasts and the antibacterial properties were demonstrated successfully against the keystone periodontal bacteria Porphyromonas gingivalis, which has an influence on implant failure, without compromising in vitro biocompatibility. In this study, PLGA was successfully modified to obtain a localised and temporally controlled drug delivery system, demonstrating the potential value of LbL as a coating technology for the manufacture of me...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

et al. 2015

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“…The proliferation of these bacteria should be controlled on the site of GBR membrane restoration to ensure successful tissue migration, growth and proliferation 7) . Several antibiotics such as tetracycline hydrochloride and metronidazole benzoate have been used extensively to overcome this problem 5,8) . However, in a view to prevent antibiotic resistance, alternative antibacterial agents are of interest to be incorporated into the GBR membrane 9,10) .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial efficacy of triple-layered poly(lactic-<i>co</i>-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria

et al. 2017

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A guided bone regeneration (GBR) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. One of the main challenges of GBR restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. Therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered GBR membrane composed of poly(lactic-co-glycolic acid) (PLGA), nanoapatite (NAp) and lauric acid (LA) with two types of Gram-negative periodontal bacteria, Fusobacterium nucleatum and Porphyromonas gingivalis through a disc diffusion and bacterial count tests. The membranes exhibited a pattern of growth inhibition and killing effect against both bacteria. The increase in LA concentration tended to increase the bactericidal activities which indicated by higher diameter of inhibition zone and higher antibacterial percentage. It is shown that the incorporation of LA into the GBR membrane has retarded the growth and proliferation of Gram-negative periodontal bacteria for the treatment of periodontal disease.

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“…Used in conjunction with sound surgical technique, GBR has become a reliable and validated procedure for local periodontitis defect treatment and increasing the width and height of the alveolar ridge before implant installation and in the treatment of periimplant bone defects. In order to improve the effects of this procedure, drugs and bioactive factors have been administered along with the GBR membrane 4,5) . Naringin is a double-hydrogen flavonoid compound extracted from Rhizomadrynariae.…”

Section: Introductionmentioning

confidence: 99%

“…The materials selected for drug loading include poly lactic acid (PLA), poly(lactide-co-glycolide) (PLGA), poly(D, L-lactic acid) (PDLLA), polycaprolactone (PCL), and other biocompatible and biodegradable polymers 6,16,19,20) . Among different classes of biodegradable polymers, the thermoplastic aliphatic poly(esters), such as PLA, PLGA and so on, have generated much interest due to their favorable properties 5,[19][20][21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> evaluation of electrospun PLGA/PLLA/PDLLA blend fibers loaded with naringin for guided bone regeneration

Guo

et al. 2018

Dent. Mater. J.

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The present study was to evaluate fiber mesh loaded with naringin via electrospinning to guide bone regeneration in vitro. The naringin-loaded fiber mesh was prepared via elctrospinning of PLGA, PLLA, PDLLA blending solution with naringin. SEM showed that naringin decreased the fiber's diameter according to the concentration of naringin. After 20 days' degradation in PBS, the drugloaded fiber meshes still kept their stability with about 10% decrease in tensile strength. In vitro release experiments showed a sustained and steady naringin releasing profile with little initial burst releasing. Compared to the mats without naringin, the fiber mats loaded with naringin showed the most pronounced enhancement of cell growth when MC3T3-E1 cells were cultured on the fiber mats. The blend fiber loaded with naringin has optimized physical properties and sustained release profile in vitro. The study presents a promising fibrous mesh material for guided bone regeneration therapy.

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Release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment

Cited by 114 publications

References 25 publications

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

Antibacterial efficacy of triple-layered poly(lactic-<i>co</i>-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria

<i>In vitro</i> evaluation of electrospun PLGA/PLLA/PDLLA blend fibers loaded with naringin for guided bone regeneration

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