Release of Immunoreactive Serotonin into the Lumen of the Feline Gut in Response to Vagal Nerve Stimulation

Ahlman, Håkan; deMagistris, L.; Zinner, Michael J.; Bm, Jaffe

doi:10.1126/science.6168020

Cited by 76 publications

(31 citation statements)

References 10 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it remains unclear which 5-HT released from the enteric nervous system or EC cells plays a major role in mediating colonic motility. 5-HT is released from EC cells into the portal circulation or basolateral border of the mucosa (14), while others showed that 5-HT is released into the lumen of GI tract (1,12). Immunoelectron microscopic study revealed that aggregation of secretory granules of 5-HT was located in the apical and basolateral cytoplasm of EC cells in the rat duodenum.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats

Tsukamoto

Ariga²,

Mantyh³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

(EC) cells of the epithelial cells release 5-HT into the lumen, as well as basolateral border. However, the physiological role of released 5-HT into the lumen is poorly understood. Concentrations of 5-HT in the colonic mucosa, colonic lumen, and feces were measured by HPLC in rats. To investigate whether intraluminal 5-HT accelerates colonic transit, 5-HT and 51 Cr were administered into the lumen of the proximal colon, and colonic transit was measured. To investigate whether 5-HT is released into the lumen, we used an ex vivo model of isolated vascularly and luminally perfused rat proximal colon. To investigate whether luminal 5-HT is involved in regulating stressinduced colonic motility, the distal colonic motility was recorded under the stress loading, and a 5-HT3 receptor antagonist (ondansetron, 10 Ϫ6 M, 0.5 ml) was administered intraluminally of the distal colon. Tissue content of 5-HT in the proximal colon (15.2 Ϯ 4.3 ng/mg wet tissue) was significantly higher than that in the distal colon (3.3 Ϯ 0.7 ng/mg wet tissue), while fecal content and luminal concentration of 5-HT was almost the same between the proximal and distal colon. Luminal administration of 5-HT (10 Ϫ6 -10 Ϫ5 M) significantly accelerated colonic transit. Elevation of intraluminal pressure by 10 cmH2O significantly increased the luminal concentration of 5-HT but not the vascular concentration of 5-HT. Stress-induced stimulation of the distal colonic motility was significantly attenuated by the luminal administration of ondansetron. These results suggest that luminally released 5-HT from EC cells plays an important role in regulating colonic motility in rats. enterochromaffin cells; distal colon; luminal pressure; restraint stress SEROTONIN (5-HT) IN THE GASTROINTESTINAL (GI) tract is involved in regulating GI motility. While 5-HT acts as a neurotransmitter of the enteric nervous system (15), the majority of 5-HT is stored in enterochromaffin (EC) cells of epithelial cells.EC cells have been considered to release 5-HT mainly into the blood vessels and/or intrinsic nerve terminal via a basolateral border (14). In contrast, others showed that 5-HT is also released into the intestinal lumen in response to vagal nerve stimulation (1,20), luminal acidification (25), and food intake (10). Immunoelectron microscopic study showed the anatomical evidence that 5-HT is released from EC cells in response to an increase of luminal pressure of the rat duodenum (12). However, the physiological role of released 5-HT into the GI lumen is poorly understood.In this study, we hypothesize that luminally released 5-HT from EC cells of the proximal colon is transferred distally with feces and stimulates motility of the distal colon, resulting in acceleration of colonic transit in rats. We also investigated the mechanism of luminal release of 5-HT of the rat proximal colon.There is accumulated evidence that a variety of stress stimulates colonic motility, colonic transit, and fecal pellet output (9,21,35,38,47). Stress-induced acceleration of colonic motor function...

show abstract

Section: Discussionmentioning

confidence: 99%

“…In contrast, others showed that 5-HT is also released into the intestinal lumen in response to vagal nerve stimulation (1,20), luminal acidification (25), and food intake (10). Immunoelectron microscopic study showed the anatomical evidence that 5-HT is released from EC cells in response to an increase of luminal pressure of the rat duodenum (12).…”

mentioning

confidence: 99%

Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats

Tsukamoto

Ariga²,

Mantyh³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…In these studies the mean perfusate concentration of 5-HT washed out to 60±60 ng/ml at the end of period I, peaked at 176.2±14.3 ,g/ml during period II, and returned to 3.8±1.7 ,ug/ml at the end of period (3,4,16,17). We have previously demonstrated, in a similar feline system, that electrical vagal nerve stimulation causes up to 20-fold elevations in the quantity of 5-HT and SP measured in saline-infused segments of proximal jejunum (1)(2)(3). In this study the effects of exogenously introduced 5-HT and SP on local blood flow were studied in the proximal feline jejunum.…”

Section: Resultsmentioning

confidence: 99%

Local regulation of blood flow in the feline jejunum. A possible role for endoluminally released substance P.

Yeo

Jaffe

Zinner³

1982

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Serotonin and substance P of gastrointestinal origin have been measured by radioimmunoassay in the bowel lumen under basal and stimulated conditions. To investigate the possibility that local blood flow may be influenced by these endoluminal hormones, 26 cats were studied with exogenous serotonin and substance P infused endoluminally into isolated proximal jejunal segments in vivo. Regional blood flow was measured by using the radioactive microsphere technique before, during, and after the endoluminal instillation of two doses of substance P (3.9 and 30 ng/min) or serotonin (0.9 and 21 ,ug/min). Neither dose of substance P changed systemic blood pressure.

show abstract

“…It would be appear that serotonin is released from the enterochromaffin cells into both the circulation and the intestinal lumen (Ahlman et al, 1981;Gronstad et al, 1984) and the mechanisms for these releases have been shown to be independent in both cases (Money et aL, 1988). Furthermore, the release of serotonin seems to be regulated by either cholinergic, adrenergic and/or peptidergic nerves as well as by intraluminal stimuli (Ferrara et al, 1987;Gronstad et al, 1987).…”

Section: Serotonin (5-hydroxytryptamine) (5 Ht)mentioning

confidence: 99%

Effect of serotonin on D-galactose transport across the rabbit jejunum

Arruebo¹,

Mesonero²,

Murillo³

et al. 1989

Reprod. Nutr. Dévelop.

View full text Add to dashboard Cite

Summary ― The patterns of storage and release of serotonin found in the enterochromaffin cells of the intestinal mucosa suggest that this hormone may be an important modulator of intestinal functions. Serotonin has been shown to produce secretion of water and electrolytes in rabbit ileum, but the hormone does not appear to interact significantly with other transport processes. The aim of the present study was to determine the effect of serotonin on D-galactose absorption in rabbit jejunum. The results obtained show that serotonin (10--8 and 10--6 M) partially reduced (by 20 and 40 % respectively) D-galactose uptake across the mucosal border. This effect was concentrationdependent, and it seemed to be caused by the inhibition of Na+-dependent sugar transport. Methysergide, an antagonist of serotonin which binds with receptor 2 of serotonin, blocked the effect of serotonin. These findings suggest that serotonin may act as a regulator of sugar intestinal absorption, and that this serotonin regulation could be mediated by a direct or indirect action of the complex serotonin―receptor, which may inhibit the Na+-dependent transport system of sugars located in the brush-border membrane.serotonin -D-galactose -intestinal transport -rabbit Résumé ― Effet de la sérotonine sur le transport du D-galactose dans le jéjunum du lapin. La sérotonine est une hormone gastro-intestinale qui se trouve principalement dans les cellules entérochromaffines de la muqueuse intestinale. L'étude du stockage comme de la libération de la sérotonine dans le tractus gastro-intestinal, a suggéré que la sérotonine peut agir comme modulateur des fonctions intestinales. La sérotonine produit la sécrétion d'eau ainsi que des électrolytes dans I iléum du lapin, mais cette hormone n'a montré aucune interaction significative avec les autres processus de transport intestinal. Le but de ce travail a été de déterminer l'effet de la sérotonine sur l'absorption du D-galactose dans le jéjunum du lapin. Les résultats obtenus montrent que la sérotonine (10-e et 10-6 M) a diminué partiellement (20 et 40 % respectivement) l'entrée du D-galactose dans la muqueuse. Cet effet dépend du degré de concentration de la sérotonine et il nous semble qu'il pourrait être une inhibition du transport des sucres dépendant du Na+. La métisergide, un antagoniste de la sérotonine qui se lie au récepteur 2 de la sérotonine, a inhibé l'effet de la sérotonine. Ces résultats suggèrent que la sérotonine pourrait jouer une fonction régulatrice sur l'absorption intestinale des sucres et que cette régulation pourrait aussi être médiée par une action directe ou indirecte du complexe récepteur-sérotonine inhibé par le système de transport des sucres dépendant du Na+ situé dans la membrane de la bordure en brosse.sérotonine -D-galactose -transport intestinal -lapin

show abstract

Release of Immunoreactive Serotonin into the Lumen of the Feline Gut in Response to Vagal Nerve Stimulation

Cited by 76 publications

References 10 publications

Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats

Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats

Local regulation of blood flow in the feline jejunum. A possible role for endoluminally released substance P.

Effect of serotonin on D-galactose transport across the rabbit jejunum

Contact Info

Product

Resources

About