Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics

Zeng, Qian; Nguyen, Sean; Zhang, Hongming; Chebrolu, Hari Priya; Alzebdeh, Dalia; Badi, Mustafa A.; Kim, Jong Ryul; Ling, Junqi; Yang, Maobin

doi:10.1016/j.joen.2016.04.029

Cited by 65 publications

(92 citation statements)

References 47 publications

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“…Zeng et al . () measured the amount of released growth factors using a similar root segment model and, reported a significant amount of TGF‐β1 was released into the root canal space even after irrigation with NaOCl followed by EDTA, and growth factors released into root canal space induced dental pulp stem cell migration. Another study using tooth slice models also demonstrated that significant amounts of TGF‐β1 were released after EDTA irrigation compared to NaOCl (Gonçalves et al .…”

Section: Discussionmentioning

confidence: 99%

“…Previous study with root segment models simulating the clinical scenario indicated that the current irrigation protocols involving sodium hypochlorite (NaOCl) and EDTA released a significant amount of TGF‐β1 and induced dental pulp stem cell migration (Zeng et al . ). Historically, there are many other solutions such as citric acid and phosphoric acid, which were used to remove smear layer during root canal treatment (Violich & Chandler ).…”

Section: Introductionmentioning

confidence: 97%

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Release of TGF‐β1 into root canals with various final irrigants in regenerative endodontics: an in vitro analysis

Chae¹,

Yang

Kim

2018

Int Endodontic J

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Release of TGF‐β1 into root canals with various final irrigants in regenerative endodontics: an in vitro analysis

Chae¹,

Yang

Kim

2018

Int Endodontic J

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“…) to 5 min (Zeng et al . ). The polyurethane film has been used to avoid EDTA effecting the pulpal cells.…”

Section: Discussionmentioning

confidence: 97%

EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth

Tziafas

Kodonas²,

Gogos³

et al. 2018

Int Endodontic J

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Aim To investigate pulp responses after pulpotomy and EDTA conditioning of pulp chamber dentinal walls with or without the placement of a collagenous scaffold in the experimental model of miniature swine teeth. Methodology Forty‐two fully developed permanent premolars and molars of healthy miniature swines were used. After preparation of pulp exposures through Class I cavities, the tissue of the pulp chamber was completely removed. The haemorrhage was controlled, and the root pulp was protected using a polyurethane film. The circumpulpal pulp chamber dentine was treated for 3 min with normal saline (group 1), or 17% EDTA solution (groups 2 and 3). The film was removed, and the pulp chamber cavities were left empty (groups 1 and 2), or filled with swine collagenous sponge (group 3). The access cavities were restored with a Teflon disc and glass ionomer. Teeth were evaluated histo‐morphologically after 10 weeks. Data were compared using the nonparametric Fisher's exact test. Results Teeth after treatment of dentine with saline (group 1) were associated with no or only traces of hard tissue formation along the root canal walls. Atubular tertiary dentine deposition in the form of matrix deposition along root canal walls, or dentine bridge formation at the orifice of root canals or complete pulp canal obliteration, was found after treatment of dentine with EDTA in both experiments (groups 2 and 3). Significantly different types of mineralization in the root canals of groups 2 and 3 were seen (P = 0.001). Tissue changes in the pulp cavity, characterized by soft tissue growth and osteodentine or atubular tertiary dentine formation, were only seen after EDTA conditioning of dentine, in 6.2% of the teeth without scaffold and 64.7% of the teeth with scaffold application. Newly deposited mineralized matrix in the pulp chamber was always in continuation with hard tissue deposited in the root canals. Conclusions The EDTA conditioning of pulp cavity dentinal walls after pulpotomy induced dentinogenic events in the root pulp. Application of collagenous scaffold in the pulp chamber enhanced soft tissue growth and mineralized tissue formation along the treated circumpulpal dentine.

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“…Furthermore, it has been realized that MMPs are present in the dentin proper, but it has not necessarily been evaluated to which extent or proportion MMPs are immobilized in the hard tissue phase, attached to the collagen, or are dissolved in the dentinal fluid [Vidal et al, 2014]. This could be done in future studies by centrifuging dentin samples to collect the fluid [Geraldeli et al, 464 2012], followed by demineralization to assess the fraction that is embedded in the mineral phase [Zeng et al, 2016].…”

Section: Discussionmentioning

confidence: 99%

MMP-9 in Dentinal Fluid Correlates with Caries Lesion Depth

et al. 2017

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The analysis of molecular cues in dentinal fluid from an excavated cavity could improve diagnostics in the context of minimally invasive caries treatment. In the current clinical trial we assessed whether the dentinal fluid levels of MMP-9 (matrix metalloproteinase-9; neutrophil gelatinase) would increase with the progression of carious lesions. MMP-9 is associated with neutrophil-related tissue breakdown in the pulp. Absolute MMP-9 levels were contrasted against the levels of MMP-2, an enzyme related to normal tissue turnover. Dentinal fluid was collected below deep and shallow caries from molars and premolars within the same patients aged 18 years and older (n = 30, 1 tooth per group/patient). Experimental teeth were isolated under a rubber dam prior to excavation. Dentinal fluid was collected from the bottom of the cavity using a size 25 paper point. MMP levels were assessed using an enzyme-linked immunosorbent assay. Nonparametric methods were applied to test for differences between groups. Significantly more (p < 0.05, Wilcoxon test) MMP-9 was collected from the deep carious lesions than from the shallow counterparts. Pairwise comparison of MMP-9 values within patients revealed that there was more MMP-9 collected from deep lesions than from shallow counterparts in 27 of the 30 individuals under investigation (pairwise Wilcoxon test, p < 0.001). In contrast, no such difference existed for MMP-2. There was a high correlation between MMP-9 from deep and shallow lesions (Spearman's ρ = 0.72, p < 0.001), indicating that patients with more MMP-9 in the deep carious lesion also tended to have more MMP-9 in the shallow lesion.

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Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics

Cited by 65 publications

References 47 publications

Release of TGF‐β1 into root canals with various final irrigants in regenerative endodontics: an in vitro analysis

Release of TGF‐β1 into root canals with various final irrigants in regenerative endodontics: an in vitro analysis

EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth

MMP-9 in Dentinal Fluid Correlates with Caries Lesion Depth

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