Release of Glycoprotein (GP1) from the Tegumental Surface of<i>Taenia solium</i>by Phospholipase C from<i>Clostridium perfringens</i>Suggests a Novel Protein-Anchor to Membranes

Landa, Abraham; Willms, Kaethe; Laclette, Juan Pedro

doi:10.1155/2010/910375

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…In addition to these carbohydrates, an additional residue, sialic acid, has been reported on membrane surface in T. solium cysticerci (Landa et al 2010). In contrast to the strong reaction for sialic acid described in the cysticerci surface, we found on the oncosphere that this carbohydrate, although present, only had a weak reactivity.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity

et al. 2013

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This study examines the carbohydrate composition of Taenia solium whole oncosphere antigens (WOAs), in order to improve the understanding of the antigenicity of the T. solium. Better knowledge of oncosphere antigens is crucial to accurately diagnose previous exposure to T. solium eggs and thus predict the development of neurocysticercosis. A set of seven lectins conjugates with wide carbohydrate specificity were used on parasite fixations and somatic extracts. Lectin fluorescence revealed that D-mannose, D-glucose, D-galactose and N-acetyl-D-galactosamine residues were the most abundant constituents of carbohydrate chains on the surface of T. solium oncosphere. Lectin blotting showed that post-translational modification with N-glycosylation was abundant while little evidence of O-linked carbohydrates was observed. Chemical oxidation and enzymatic deglycosylation in situ were performed to investigate the immunoreactivity of the carbohydrate moieties. Linearizing or removing the carbohydrate moieties from the protein backbones did not diminish the immunoreactivity of these antigens, suggesting that a substantial part of the host immune response against T. solium oncosphere is directed against the peptide epitopes on the parasite antigens. Finally, using carbohydrate probes, we demonstrated for the first time that the presence of several lectins on the surface of the oncosphere was specific to carbohydrates found in intestinal mucus, suggesting a possible role in initial attachment of the parasite to host cells.

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Section: Discussionmentioning

confidence: 99%

Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity

et al. 2013

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Differential antigenic protein recovery from Taenia solium cyst tissues using several detergents

Navarrete‐Perea

Orozco-Ramírez

Moguel

et al. 2015

Molecular and Biochemical Parasitology

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In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles

McVeigh

Cwiklinski

Andres

et al. 2018

Sci Rep

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Glycoproteins secreted by helminth parasites are immunogenic and represent appealing components of vaccine preparations. Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control. Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock. Using 190 human sequences as search queries against currently available F. hepatica genomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation. We found appreciable duplication within these orthologues, describing just 87 non-redundant genes when paralogues were excluded. F. hepatica lacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described by F. hepatica glycomic datasets, and predicts pervasive structural simplicity in the wider glycome. These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.

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Release of Glycoprotein (GP1) from the Tegumental Surface ofTaenia soliumby Phospholipase C fromClostridium perfringensSuggests a Novel Protein-Anchor to Membranes

Cited by 4 publications

References 26 publications

Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity

Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity

Differential antigenic protein recovery from Taenia solium cyst tissues using several detergents

In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles

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