Release of Ciprofloxacin from Chondroitin 6-Sulfate-Graft-Poloxamer Hydrogel In Vitro for Ophthalmic Drug Delivery

Yoo, Mi Kyong; Cho, K. Y.; Song, Hyun Hoon; Choi, Yun‐Jaie; Kwon, Ji-Won; Kim, M. K.; Lee, J. H.; Wee, Won Ryang; Cho, C. S.

doi:10.1080/03639040500214688

Cited by 33 publications

(19 citation statements)

References 25 publications

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“…Heparin-poloxamer (HP) was prepared according to the EDC/NHS method as previously described29. The bFGF-ASC-HP hydrogels were prepared using the cold method30.…”

Section: Methodsmentioning

confidence: 99%

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Tian

et al. 2016

Sci Rep

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Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions. The bFGF-ASC complex was further encapsulated into a heparin modified poloxamer (HP) solution to prepare atemperature-sensitive hydrogel (bFGF-ASC-HP). bFGF release from the ASC-HP hydrogel was more slower than that from the bFGF-ASC complex alone. An in vitro cell survival study showed that the bFGF-ASC-HP hydrogel could more effectively promote the proliferation of PC12 cells than a bFGF solution, with an approximate 50% increase in the cell survival rate within 24 h (P < 0.05). Compared with the bFGF solution, bFGF-ASC-HP hydrogel displayed enhanced inhibition of glial scars and obviously improved the functional recovery of the SCI model rat through regeneration of nerve axons and the differentiation of the neural stem cells. In summary, an ASC-HP hydrogel might be a promising carrier to deliver bFGF to an injured spinal cord.

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“…Heparin-poloxamer (HP) was prepared according to the EDC/NHS method as previously described29. The bFGF-ASC-HP hydrogels were prepared using the cold method30.…”

Section: Methodsmentioning

confidence: 99%

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Tian

et al. 2016

Sci Rep

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“…10 Antibodies against Bax, Bcl-2, Caspase-3, and GAPDH were obtained from Cell Signaling Technology (CST, CA, USA) and PCNA, bFGF, and CK-18 were purchased from Abcam (Abcam, CB, UK). The synthesis of poloxamer 407-grafted heparin copolymer was performed according to a previous literature.…”

Section: Methodsmentioning

confidence: 99%

“…10 Briefly, lyophilized HP powder was gently mixed and stirred with 17β-estradiol solution overnight at 4°C. 10 Briefly, lyophilized HP powder was gently mixed and stirred with 17β-estradiol solution overnight at 4°C.…”

Section: Preparation and Characterization Of E 2 -Hp Hydrogelsmentioning

confidence: 99%

Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model

Zhang

Xiang

et al. 2019

The FASEB Journal

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Mechanical damage or infection to the endometrium can lead to the formation of adhesions in the uterine cavity, which may result in reduced reproductive outcome and/ or pregnancy complications. The prognosis of this disease is poor due to few effective treatments and the complex environment of endometrium. Heparin-Poloxamer Hydrogel (HP hydrogel) is a nontoxic and biodegradable biomaterial, which has been commonly used as a sustained-release delivery system. In this study, we applied a miniendometrial curette to scrape the endometrium of rats to mimic the process of curettage in patients. After the establishment of IUA model in rats, we injected the thermo-sensitive hydrogel(E 2 -HP hydrogel) into the injured uterine cavity and evaluated the therapeutic effect of E 2 -HP hydrogel on the recovery of IUA. Our results showed that E 2 -HP hydrogel can significantly facilitate the regeneration of injured endometrium along with inhibiting the cell apoptosis in IUA model. Furthermore, we revealed that E 2 -HP hydrogel on the recovery of IUA was closely associated with the upregulation of kisspeptin through activating the ERK1/2 and MAPKs p38 pathways. In conclusion, E 2 -HP hydrogel can effectively transfer E 2 into the injured endometrium and it can be considered as a promising therapeutic method for the women with intrauterine adhesions. K E Y W O R D S17β-estradiol, apoptosis, heparin-poloxamer hydrogel, intrauterine adhesions, kisspeptin, regeneration | 447 ZHANG et Al.

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“…Hydrogels like poly(N-isopropyl acrylamide) (PNIPAM) have been grafted with chitosan to form a thermally responsive ophthalmic drug delivery device 81. Coating with chitosan has proven to be advantageous as many corneal and conjunctival cells have high affinity for chitosan 82. Hence coating with naturally occurring proteins can greatly enhance the ocular biocompatibility.…”

Section: Challenges Faced In Using Nanodevices For Intraocular Tumorsmentioning

confidence: 99%

Nanotechnology in the Treatment and Detection of Intraocular Cancers

Thevenot¹,

Hu²,

Tang³

2008

j biomed nanotechnol

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Tremendous progress in nanotechnology has lead to the development of nanometer-sized objects as medical implants or devices. Many of these nanodevices have recently been tested in many cancer diagnostic and therapeutic applications, such as leukemia, melanoma, breast tumor, prostate tumor, and brain cancer. Despite the increasing importance of nanotechnology in cancer, the potential of these nanodevices in diagnosing and treating intraocular cancers has not been systematically evaluated. This review summarizes the significant advancements and potential areas for development in the field of nanotechnology-based intraocular drug delivery and imaging. KeywordsIntraocular cancer; uveal melanoma; retinoblastoma; nanoparticles; liposomes; quantum dots; intravitreal injection; drug delivery; imaging Intraocular Cancers -Overview and detectionThough less common, the risk of complications and metastatic potential of intraocular cancers presents a very dangerous condition, warranting the same vigilant management as other cancers. Due to their proximity to critical ocular structures, early diagnosis and treatment of intraocular cancer are essential to preserve vision. There are two common forms of intraocular tumors which can be characterized based on the typical age of occurrence: ocular melanoma in adults and retinoblastoma in children. Ocular melanoma is the most common malignancy originating in the eye in older patients with a median onset age of 55. 1,2 Retinoblastoma is a very common form of ocular malignancy occurring in children. 1Uveal melanoma is the most common type of ocular melanoma and typically presents as a small tumor near critical structures in the eye. 1,3 The potential causes and risk factors of uveal melanoma remain undetermined, 6 though incidence is slightly higher in men, with rates 150 times higher in Caucasians compared to those with darker skin. This is consistent with the statistically higher incidence seen in patients with light skin, blue eyes, and blonde hair. 1 Compared with other intraocular tumors, uveal melanoma has the highest rate of metastasis, with a 40% metastasis rate (median survival 2-7 months) and approximately The most concerning issue with this condition are problems with detection and treatment. 5 As such, the rate of metastasis and mortality has remained unchanged over the years. Typically, uveal melanoma is diagnosed by binocular indirect ophthalmicroscopy. 6 Indocyanine green angiography is also used to visualize the tumor and tumor margins. 6 The most common treatment options include enucleation, local resection, brachytherapy, of which brachytherapy would represent the most common. 6 These approaches are plagued by high failure rates and complications with large tumors or those near the optic nerve, 4 with other complications including iris neovascularization and neovascular glaucoma. 7 External beam radiotherapy has success rates similar to enucleation, providing local control and organ preservation along with unmatched cosmetic results and visual preservation. 3...

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Release of Ciprofloxacin from Chondroitin 6-Sulfate-Graft-Poloxamer Hydrogel In Vitro for Ophthalmic Drug Delivery

Cited by 33 publications

References 25 publications

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model

Nanotechnology in the Treatment and Detection of Intraocular Cancers

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