Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan

Over the last decade, numerous evidences have contributed to establish a link between the natural and human-impacted environments and the growing public health threat that is the antimicrobial resistance. In the environment, in particular in areas subjected to strong anthropogenic pressures, water plays a major role on the transformation and transport of contaminants including antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes. Therefore, the urban water cycle, comprising water abstraction, disinfection, and distribution for human consumption, and the collection, treatment, and delivery of wastewater to the environment, is a particularly interesting loop to track the fate of antibiotic resistance in the environment and to assess the risks of its trans-mission back to humans. In this article, the relevance of different transepts of the urban water cycle on the potential enrichment and spread of antibiotic resistance is reviewed. According to this analysis, some gaps of knowledge, research needs, and control measures are suggested. The critical rationale behind the measures suggested and the desirable involvement of some key action players is also discussed.

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“…On the other hand, UV process did not contribute to a significant reduction of tetracycline-and sulfonamide-resistant E. coli (Munir et al 2011).…”

Section: Advanced Wastewater Treatment and The Control Of Antibiotic mentioning

confidence: 77%

Antibiotic resistance in urban aquatic environments: can it be controlled?

Manaia

Macedo

Fatta‐Kassinos

et al. 2015

Appl Microbiol Biotechnol

177

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“…This phenomenon may be due, at least partly, to potential changes in soil microbial composition including the native soil antibiotic-resistant bacteria (ARB) after the application of chemical fertilizer (Forsberg et al, 2014). Although chemical fertilizer had some effects on soil ARGs, these effects, not surprisingly, are weaker when compared with sewage sludge or chicken manure, considering that manure is rich in ARGs and ARBs , Jiang et al, 2002Ghosh and LaPara, 2007) and most ARGs are discharged from the municipal wastewater treatment process in the form of sewage sludge (Munir et al, 2011). However, land application of animal manure or sewage sludge does not universally increase ARGs in soils (Zhou et al, 2010) and this increase is not maintained long-term (Fahrenfeld et al, 2014).…”

Section: Responses Of Args Abundance and Compositionmentioning

confidence: 99%

“…Municipal wastewater appears to be a significant reservoir of ARGs (Lachmayr et al, 2009), however, most ARGs cannot be effectively removed by wastewater treatment processes. ARGs have been detected at all stages of the municipal wastewater treatment process (Lachmayr et al, 2009;Munir et al, 2011), and the vast majority of ARGs are discharged from the municipal wastewater treatment process together with sewage sludge (Bondarczuk et al, 2016). Land application is one of the key management approaches of sewage sludge from wastewater treatment plants (Kim and Aga, 2007).…”

Section: Introductionmentioning

confidence: 99%

Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil

Chen

et al. 2016

Environment International

668

246

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“…9 For example, UV processes have failed to reduce ARGs. 13 Chen and Zhang detected high concentrations of tet (2.5 × 10 4 to 1.2 × 10 4 copies/ml) and sul genes (6.6 × 10 5 to 3.1 × 10 6 copies/mL) in the effluent of different WWTPs after advanced treatment process. 9 Thus, many studies have now illustrated the occurrence of ARGs and antibiotics in wastewater, sludge, and reclaimed water.…”

Section: ■ Introductionmentioning

confidence: 99%

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Wang

Qiao

et al. 2014

Environ. Sci. Technol.

381

201

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Reclaimed water irrigation (RWI) in urban environments is becoming popular, due to rapid urbanization and water shortage. The continuous release of residual antibiotics and antibiotic resistance genes (ARGs) from reclaimed water could result in the dissemination of ARGs in the downstream environment. This study provides a comprehensive profile of ARGs in park soils exposed to RWI through a high-throughput quantitative PCR approach. 147 ARGs encoding for resistance to a broad-spectrum of antibiotics were detected among all park soil samples. Aminoglycoside and beta-lactam were the two most dominant types of ARGs, and antibiotic deactivation and efflux pump were the two most dominant mechanisms in these RWI samples. The total enrichment of ARGs varied from 99.3-fold to 8655.3-fold compared to respective controls. Six to 60 ARGs were statistically enriched among these RWI samples. Four transposase genes were detected in RWI samples. TnpA-04 was the most enriched transposase gene with an enrichment was up to 2501.3-fold in Urumqi RWI samples compared with control soil samples. Furthermore, significantly positive correlation was found between ARGs and transposase abundances, indicating that transposase might be involved in the propagation of ARGs. This study demonstrated that RWI resulted in the enrichment of ARGs in urban park soils.

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Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan

Cited by 753 publications

References 28 publications

Antibiotic resistance in urban aquatic environments: can it be controlled?

Antibiotic resistance in urban aquatic environments: can it be controlled?

Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

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