Release behaviour of amoxicillin from chitosan coated liposomes derived from eggs

Abstract

Abstract:A delivery system consisting of chitosan coated liposomes was tested for its ability to deliver drugs over a period of time. Amoxicillin was used as the drug of choice and was entrapped in liposomes made of egg yolk lecithin and coated with chitosan with the aid of Tween 80 and sodium tripolyphosphate. These nanoparticles were monitored for the release of the drug in vitro. The activity of the released amoxicillin was measured against Staphylococcus aureus (NCTC-6571 strain) in BHI broth medium. The percentage of drug released with time was measured by HPLC. The nanoparticles showed sustained release of amoxicillin over a period of 24 hours. Approximately 80 % of the encapsulated drug was released in the first 10 hours. A sufficient drug release to kill the bacteria was obtained in 4 hours and a steady increase in drug concentration was observed up to 8 hours of testing. This study has enabled the model formulation of a sustained release delivery system for amoxicillin in vitro capable of delivering the drug over a period of 8 hours, which may enable drug activity such that the number of times of administration is reduced.

Cosmetic-Derived Mannosylerythritol Lipid-B-Phospholipid Nanoliposome: An Acid-Stabilized Carrier for Efficient Gastromucosal Delivery of Amoxicillin for In Vivo Treatment of Helicobacter pylori

“…The amount of free AMX in the eluent was determined using HPLC. 22 EE was calculated using the following equation …”

“…Evaluation of in vitro drug release was performed using the dialysis diffusion method for 2.0 mL of 1 mg/mL liposome suspension (encapsulating 0.1 mg/mL AMX) in a dialysis bag (MWCO = 3.5–5 kD) under conditions previously specified, 21 , 23 and the concentration of AMX was determined by HPLC. 22 Particularly, phase-dependent drug release was evaluated. In detail, a 9 h drug release test was performed for the AMX-loaded liposomes.…”

“…Briefly, 1 mL of AMX-loaded liposomes (1 mg/mL) was placed in a 10.0 kDa Millipore ultrafiltration tube (Millipore, USA), followed by centrifugation at 10,000 g for 10 min. The amount of free AMX in the eluent was determined using HPLC . EE was calculated using the following equation …”

“…The in vitro pH stability of liposomes was determined by placing them in 20 mM PBS buffer of different pH (3.0–7.0) values at 25 °C for 12 h. The particle sizes of the treated liposomes were measured using Zetasizer Nano S90 (Malvern, UK). Evaluation of in vitro drug release was performed using the dialysis diffusion method for 2.0 mL of 1 mg/mL liposome suspension (encapsulating 0.1 mg/mL AMX) in a dialysis bag (MWCO = 3.5–5 kD) under conditions previously specified, , and the concentration of AMX was determined by HPLC . Particularly, phase-dependent drug release was evaluated.…”

The state of nanomedicine in Sri Lanka: challenges and opportunities

“…Many Sri Lankan scientists have reported a sizable amount of work related to anticancer drug delivery systems. Most of these drug delivery systems are based on chitosan alginate nanoparticles (Katuwavila et al, 2016a;Katuwavila et al, 2016b) or liposomes (Samantha Dissanayake et al, 2015;Pamunuwa et al, 2016;Pamunuwa et al, 2015;Menikarachchi et al, 2016;Nwokwu et al, 2017). They have been tested on various cancer cell lines such as breast (Katuwavila et al, 2016a) and lung (Nwokwu et al, 2017) cancer.…”