Release behavior and antibiofilm activity of usnic acid-loaded carboxylated poly(l-lactide) microparticles

Martinelli, Andrea; Bakry, Ahmed; D’Ilario, L.; Francolini, Iolanda; Piozzi, Antonella; Taresco, Vincenzo

doi:10.1016/j.ejpb.2014.06.002

Cited by 43 publications

(29 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…)-usnic acid, loaded into magnetic polylactic-co-glycolic acid-polyvinyl alcohol (PLGA-PVA) microspheres, inhibited not only the initial attachment of S. aureus to the coated surface, but also the development of mature biofilms (Grumezescu et al 2014). Similar studies by Martinelli et al (2014) revealed that (? )-usnic acidloaded into carboxylated poly(L-lactide) microparticles was able to inhibit 24-h Staphylococcus epidermidis biofilm, with results that were more profound than the use of (?…”

Section: Antibacterial Activity and Antibiofilm Propertiesmentioning

confidence: 95%

Enantioselective activity of usnic acid: a comprehensive review and future perspectives

2019

View full text Add to dashboard Cite

This review is focused on the comparison of the biological and pharmacological activities of usnic acid enantiomers. Most of the available data refer to (?)-usnic acid, while the left-handed isomer has been less often significantly studied. Special attention was paid to the experiments comparing both (?)-and (-)-usnic acid at the same time, the results of which indicated interesting differences, however no tendency as to which enantiomer was more potent could be observed. Nevertheless, more studies, especially on (-)-usnic acid, are needed to give a final explanation for the similarities and differences between both usnic acid enantiomers. These should be especially directed to steric structure-activity relationship of the enantiomers, tested under the same experimental conditions, which may help to explain the possible mechanisms of their actions.

show abstract

Section: Antibacterial Activity and Antibiofilm Propertiesmentioning

confidence: 95%

Enantioselective activity of usnic acid: a comprehensive review and future perspectives

2019

View full text Add to dashboard Cite

show abstract

“…In the spectrum of APLLA sc , the absorption peaks at 1444 cm −1 (CH 3 bending), 1755 cm −1 (CO stretch, ester and acid groups), 2875 cm −1 (CH stretch), and 2950 (CH 3 stretch) are the characteristic peaks corresponding to PLLA . The other peaks at 1616 cm −1 (Amide I, CO stretching) and at 1542 cm −1 (Amide II, NH 2 bending), in addition to the broad band (3100–3700 cm −1 ) with a weak intensity and centered at 3400 cm −1 (NH stretching) assured the surface aminolysis reaction …”

Section: Resultsmentioning

confidence: 97%

Synergistic effects of surface grafting with heparin and addition of poly(d,l‐lactide) microparticles on properties of poly(l‐lactide) single crystals scaffolds

Bakry

2019

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Mixtures of heparin grafted poly(l‐lactide) single crystals (Hep‐PLLAsc) and poly(d,l‐lactide) (PDLLA) microparticles are used for assembling hydrophilic and cytocompatible three‐dimensional tissue engineering scaffolds simply by compression molding/salt leaching technique. PLLA is grafted with heparin to promote its surface cytocompatability, while PDLLA is added to reinforce the scaffolds. PLLAsc are aminolyzed with tetraethylenepentamine (13‐atom spacer) to generate sterically accessible amino groups at the surface allowing the covalent attachment of heparin by aqueous carbodiimide chemistry. Morphological and compressive strength studies manifest the integrity and compactness of scaffolds. Toluidine blue assay assures the consistency of heparin distribution throughout the scaffolds, which is in contrary to the conventional grafting reactions by immersing the scaffold in heparin solution. The scaffolds applicability is examined by fibroblast cells seeding experiments. Contrary to pristine scaffolds, Hep‐PLLAsc scaffolds display a hydrophilic and cytocompatible interface for cell adhesion, spreading, growth, and migration. Therefore, combining presurface grafted PLLAsc with PDLLA microparticles could offer durable scaffolds exhibiting bioactive interface and controllable spatial distribution of the grafted biomacromolecules. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47797.

show abstract

“…This has recently been tested with cefotaxime-impregnated chitosan particles [192] and farnesol-loaded cationic nanoparticles [193]. Polymer microparticles and magnetic nanoparticles also yielded positive results as carriers for usnic acid in vitro antimicrobial and anti-biofilm assays [194,195].…”

Section: Discussionmentioning

confidence: 99%

Fungal Metabolites for the Control of Biofilm Infections

Estrela

Abraham

2016

Agriculture

View full text Add to dashboard Cite

Many microbes attach to surfaces and produce a complex matrix of polymers surrounding their cells, forming a biofilm. In biofilms, microbes are much better protected against hostile environments, impairing the action of most antibiotics. A pressing demand exists for novel therapeutic strategies against biofilm infections, which are a grave health wise on mucosal surfaces and medical devices. From fungi, a large number of secondary metabolites with antimicrobial activity have been characterized. This review discusses natural compounds from fungi which are effective against fungal and bacterial biofilms. Some molecules are able to block the cell communication process essential for biofilm formation (known as quorum sensing), others can penetrate and kill cells within the structure. Several targets have been identified, ranging from the inhibition of quorum sensing receptors and virulence factors, to cell wall synthesizing enzymes. Only one group of these fungal metabolites has been optimized and made it to the market, but more preclinical studies are ongoing to expand the biofilm-fighting arsenal. The broad diversity of bioactive compounds from fungi, their activities against various pathogens, and the multi-target trait of some molecules are promising aspects of fungal secondary metabolites. Future screenings for biofilm-controlling compounds will contribute to several novel clinical applications.

show abstract

Release behavior and antibiofilm activity of usnic acid-loaded carboxylated poly(l-lactide) microparticles

Cited by 43 publications

References 49 publications

Enantioselective activity of usnic acid: a comprehensive review and future perspectives

Enantioselective activity of usnic acid: a comprehensive review and future perspectives

Synergistic effects of surface grafting with heparin and addition of poly(d,l‐lactide) microparticles on properties of poly(l‐lactide) single crystals scaffolds

Fungal Metabolites for the Control of Biofilm Infections

Contact Info

Product

Resources

About